Frontiers in Pharmacology,
Journal Year:
2023,
Volume and Issue:
14
Published: Nov. 15, 2023
Introduction:
Non-small
cell
lung
cancer
(NSCLC)
exhibits
heterogeneity
with
diverse
immune
infiltration
patterns
that
can
influence
tumor
behavior
and
immunotherapy.
A
comprehensive
characterization
of
the
microenvironment
guide
precision
medicine.
Methods:
Here,
we
generated
a
single-cell
atlas
398170
cells
from
52
NSCLC
patients,
investigated
imprinted
genes
cellular
crosstalk
for
macrophages.
Subsequently,
evaluated
effect
on
macrophages
verified
expression
marker
using
co-culture
experiments,
flow
cytometry
RT-qPCR
assays.
Results:
Remarkable
macrophage
adaptability
to
environment
was
observed,
which
contributed
generating
tumor-associated
(TAMs).
We
identified
5
distinct
functional
TAM
subtypes,
majority
were
SELENOP-positive
macrophages,
high
levels
SLC40A1
CCL13.
The
TAMs
also
involved
in
mediating
CD8+
T
activity
form
intercellular
interaction
cells,
as
indicated
by
receptor-ligand
binding.
Indirect
coculture
SPC-A1
THP-1
monocytes,
produced
M2-like
highly
expressed
several
markers
abundance
this
type
seemed
be
associated
poorer
overall
survival
rates
[hazard
ratio
(HR)
=
1.34,
95%
confidence
interval
(CI)
0.98-1.83,
p
0.068]
based
deconvolution
TCGA-LUAD
dataset.
Discussion:
In
summary,
provided
high-resolution
molecular
resource
TAMs,
displayed
acquired
properties
microenvironment.
Dynamic
between
via
multiple
ligand-receptor
pairs
revealed,
emphasizing
its
role
sustaining
pro-tumoral
implications
therapy.
Nano Letters,
Journal Year:
2024,
Volume and Issue:
24(5), P. 1717 - 1728
Published: Jan. 25, 2024
Surgery
is
the
primary
method
to
treat
malignant
melanoma;
however,
residual
microtumors
that
cannot
be
resected
completely
often
trigger
tumor
recurrence,
causing
tumor-related
mortality
following
melanoma
resection.
Herein,
we
developed
a
feasible
strategy
based
on
combinational
chemoimmunotherapy
by
cross-linking
carboxymethyl
chitosan
(CMCS)-originated
polymetformin
(PolyMetCMCS)
with
cystamine
prepare
stimuli-responsive
nanogel
(PMNG)
owing
disulfide
bond
in
can
cleaved
massive
glutathione
(GSH)
sites.
Then,
chemotherapeutic
agent
doxorubicin
(DOX)
was
loaded
PMNG,
which
followed
hyaluronic
acid
coating
improve
overall
biocompatibility
and
targeting
ability
of
prepared
(D@HPMNG).
Notably,
PMNG
effectively
reshaped
immune
microenvironment
reprogramming
tumor-associated
macrophage
phenotypes
recruiting
intratumoral
CD8+
T
cells
inherited
immunomodulatory
capability
metformin.
Consequently,
D@HPMNG
treatment
remarkably
suppressed
growth
inhibited
its
recurrence
after
surgical
resection,
proposing
promising
solution
for
overcoming
lethal
recurrence.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(27)
Published: April 21, 2024
Abstract
Therapy‐induced
immunogenic
cell
death
(ICD)
can
initiate
both
innate
and
adaptive
immune
responses
for
amplified
anti‐tumor
efficacy.
However,
dying
cell‐released
ICD
signals
are
prone
to
being
sequestered
by
the
TIM‐3
receptors
on
dendritic
(DC)
surfaces,
preventing
surveillance.
Herein,
dismantlable
coronated
nanoparticles
(NPs)
fabricated
as
a
type
of
spatiotemporally
controlled
nanocarriers
coupling
tumor
cell‐mediated
induction
DC‐mediated
sensing.
These
NPs
loaded
with
an
inducer,
mitoxantrone
(MTO),
wrapped
redox‐labile
anti‐TIM‐3
(αTIM‐3)
antibody
corona,
forming
separable
core–shell
structure.
The
corona
disintegrates
under
high
levels
extracellular
reactive
oxygen
species
in
microenvironment,
exposing
MTO‐loaded
NP
core
releasing
functional
αTIM‐3
molecules
DC
sensitization.
Systemic
administration
augments
maturation,
promotes
cytotoxic
T
recruitment,
enhances
susceptibility
checkpoint
blockade,
prevents
side
effects
MTO.
This
study
develops
promising
nanoplatform
unleash
potential
host
immunity
cancer
therapy.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Oct. 18, 2024
Nanocatalytic
immunotherapy
holds
excellent
potential
for
future
cancer
therapy
due
to
its
rapid
activation
of
the
immune
system
attack
tumor
cells.
However,
a
high
level
N-glycosylation
can
protect
cells,
compromising
anticancer
immunity
nanocatalytic
immunotherapy.
Here,
we
show
2-deoxyglucose
(2-DG)
and
bismuth
ferrite
co-loaded
gel
(DBG)
scaffold
enhanced
piezocatalytic
After
implantation
in
tumor,
DBG
generates
both
reactive
oxygen
species
(ROS)
piezoelectric
signals
when
excited
with
ultrasound
irradiation,
significantly
promoting
immunity.
Meanwhile,
2-DG
released
from
ROS-sensitive
disrupts
N-glycans
synthesis,
further
overcoming
immunosuppressive
microenvironment
tumors.
The
synergy
effects
ultrasound-triggered
glycosylation
inhibition
are
demonstrated
on
four
mouse
models.
A
"hot"
tumor-immunity
niche
is
produced
inhibit
progress
lung
metastasis
elicit
strong
memory
effects.
This
work
provides
promising
malignant
solid
tumors
featuring
low
immunogenicity
levels
N-glycosylation.
Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
19(4), P. 042003 - 042003
Published: May 13, 2024
Abstract
Infectious
diseases
severely
threaten
human
health,
and
traditional
treatment
techniques
face
multiple
limitations.
As
an
important
component
of
immune
cells,
macrophages
display
unique
biological
properties,
such
as
biocompatibility,
immunocompatibility,
targeting
specificity,
immunoregulatory
activity,
play
a
critical
role
in
protecting
the
body
against
infections.
The
macrophage
membrane-coated
nanoparticles
not
only
maintain
functions
inner
but
also
inherit
characteristics
macrophages,
making
them
excellent
tools
for
improving
drug
delivery
therapeutic
implications
infectious
(IDs).
In
this
review,
we
describe
their
advantages
challenges
ID
therapy.
We
first
summarize
pathological
features
IDs,
providing
insight
into
how
to
fight
them.
Next,
focus
on
classification,
characteristics,
preparation
nanoparticles.
Finally,
comprehensively
progress
combating
including
delivery,
inhibition
killing
pathogens,
modulation.
At
end
look
forward
aspect
is
presented.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(50)
Published: Oct. 27, 2023
Macrophage
phagocytosis
of
tumor
cells
has
emerged
as
an
attractive
strategy
for
therapy.
Nevertheless,
immunosuppressive
M2
macrophages
in
the
microenvironment
and
high
expression
anti-phagocytic
signals
from
impede
therapeutic
efficacy.
To
address
these
issues
improve
management
malignant
tumors,
this
study
we
developed
a
gene-editable
palladium-based
bioorthogonal
nanoplatform,
consisting
CRISPR/Cas9
gene
editing
system-linked
Pd
nanoclusters,
hyaluronic
acid
surface
layer
(HBPdC).
This
HBPdC
nanoplatform
exhibited
satisfactory
tumor-targeting
efficiency
triggered
Fenton-like
reactions
to
generate
reactive
oxygen
species
chemodynamic
therapy
macrophage
M1
polarization,
which
directly
eliminated
cells,
stimulated
antitumor
response
macrophages.
could
reprogram
through
reduce
CD47
adipocyte
plasma
membrane-associated
protein,
thereby
promoting
their
recognition
by
Moreover,
induced
activation
sequestered
prodrugs
via
catalysis,
enabling
chemotherapy
enhancing
cell
death.
Importantly,
nanoclusters
were
sufficiently
cleared
basic
metabolic
pathways,
confirming
biocompatibility
biosafety.
Therefore,
phagocytosis,
system
herein
represents
highly
promising
toolset
cancer
applications.
