Urinary TYROBP and HCK as genetic biomarkers for non-invasive diagnosis and therapeutic targeting in IgA nephropathy DOI Creative Commons

Boji Xie,

Shuting Pang,

Yuli Xie

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Background IgA nephropathy (IgAN) is a leading cause of renal failure, but its pathogenesis remains unclear, complicating diagnosis and treatment. The invasive nature biopsy highlights the need for non-invasive diagnostic biomarkers. Bulk RNA sequencing (RNA-seq) urine offers promising approach identifying molecular changes relevant to IgAN. Methods We performed bulk RNA-seq on 53 samples from 11 untreated IgAN patients healthy controls, integrating these data with public RNA-seq, microarray, scRNA-seq datasets. Machine learning was used identify key differentially expressed genes, protein expression validated by immunohistochemistry (IHC) drug-target interactions explored via docking. Results Urine analysis revealed differential profiles, which TYROBP HCK were identified as biomarkers using machine learning. These in both test cohort an external validation cohort, demonstrating strong predictive accuracy. confirmed their cell-specific patterns, correlating function metrics such GFR serum creatinine. IHC further expression, docking suggested potential therapeutic treatments. Conclusion are urinary Their accuracy, through learning, along confirmation insights, supports applications

Language: Английский

Energy-Confinement 3D Flower-Shaped Cages for AI-Driven Decoding of Metabolic Fingerprints in Cardiovascular Disease Diagnosis DOI
Zhiyu Li, Shuyu Zhang, Qianfeng Xiao

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Rapid and accurate detection plays a critical role in improving the survival prognosis of patients with cardiovascular disease, but traditional methods are far from ideal for those suspected conditions. Metabolite analysis based on nanomatrix-assisted laser desorption/ionization mass spectrometry (NMALDI-MS) is considered to be promising technique disease diagnosis. However, performance core nanomatrixes has limited its clinical application. In this study, we constructed 3D flower-shaped cages controllable structured metal-organic frameworks iron oxide nanoparticles low thermal conductivity significant photothermal effects. The elongation incident light path through multilayer reflection significantly enhances effective absorption area nanomatrixes. Concurrently, alternating layered structure confines energy, reducing losses. Moreover, increases affinity sites, expanding coverage. This approach effectively ionization desorption efficiency during LDI process. We applied technology analyze serum metabolomes myocardial infarction, heart failure, failure combined achieving cost-effective, high-throughput, highly accurate, user-friendly diseases. Subsequently, deep detected fingerprints via artificial intelligence models screens potential metabolic biomarkers, providing new paradigm diagnosis

Language: Английский

Citations

2
Fusion of Exosomes and Nanotechnology: Cutting-Edge Cancer Theranostics DOI

R. Agnihotram,

Rajib Dhar,

Debolina Dhar

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 8489 - 8506

Published: April 15, 2024

Cancer is the most challenging health complication worldwide. Scientific society has tried to decode its complications and developing a smart solution for mankind. Extracellular vesicles (EVs) are an emerging frontier in cancer research. EVs provide fresh insight into understanding of released from all active cells. classified some major classes, such as apoptotic bodies, microvesicles, exosomes. Exosomes among exciting tools current research they work cellular messengers (which reflects cell's healthy or pathological conditional status). In tumor microenvironment, regulate several complicated events uncontrolled cell growth, immune suppression, angiogenesis, extracellular matrix remodeling, metastasis, organ-specific drug therapeutic resistance, stem development. also source promising biomarkers involved both diagnosis prognosis performs role. Exosomes-based nanomedicine which application nanosize theranostics sensor orientation, supports overcoming management limitations, early therapeutics specific, effective, affordable. this review, we have highlighted integration exosomes nanotechnology cutting-edge approach precision oncology.

Language: Английский

Citations

14
Two-dimensional nanomaterials-based optical biosensors empowered by machine learning for intelligent diagnosis DOI

Rongshuang Tang,

Jianyu Yang,

Changzhuan Shao

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118162 - 118162

Published: Feb. 1, 2025

Language: Английский

Citations

1
LncRNA BCYRN1 as a Potential Therapeutic Target and Diagnostic Marker in Serum Exosomes in Bladder Cancer DOI Open Access
Junya Arima, Hirofumi Yoshino,

Wataru Fukumoto

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5955 - 5955

Published: May 29, 2024

Bladder cancer (BC) is a common genitourinary malignancy that exhibits silent morbidity and high mortality rates because of lack diagnostic markers limited effective treatments. Here, we evaluated the role lncRNA brain cytoplasmic RNA 1 (BCYRN1) in BC. We performed loss-of-function assays to examine effects BCYRN1 downregulation T24 BOY BC cells. found significantly inhibited proliferation, migration, invasion, three-dimensional spheroid formation ability induced apoptosis Additionally, gene set enrichment analysis (GSEA) using sequences from tumor fractions showed decreased expression mRNAs associated with cell cycle. These findings were supported by observations G2/M arrest flow cytometry assays. Finally, examined serum exosomal as biomarker. Clinically, exosomes patients (n = 31) was higher than healthy donors 19; mean difference: 4.1-fold higher, p < 0.01). Moreover, who had undergone complete resection BC, levels 8). Thus, may be promising marker therapeutic target

Language: Английский

Citations

7
Construction of a sensitive SWCNTs integrated SPR biosensor for detecting PD-L1+ exosomes based on Fe3O4@TiO2 specific enrichment and signal amplification DOI

Hezhen Liu,

Yangyang Zhou, Weiwei Chang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 262, P. 116527 - 116527

Published: June 25, 2024

Language: Английский

Citations

7
Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry DOI
Yi Zhang,

Bokai Zhou,

Qiuning Li

et al.

Chemical Research in Chinese Universities, Journal Year: 2024, Volume and Issue: 40(2), P. 237 - 254

Published: March 20, 2024

Language: Английский

Citations

5
Magnetic 3D macroporous MOF oriented urinary exosome metabolomics for early diagnosis of bladder cancer DOI Creative Commons

Yiqing Cao,

Jianan Feng,

Qiao Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 2, 2024

Bladder cancer (BCa) exhibits the escalating incidence and mortality due to untimely inaccurate early diagnosis. Urinary exosome metabolites, carrying critical tumor cell information directly related bladder, emerge as promising non-invasive diagnostic biomarkers of BCa. Herein, magnetic 3D ordered macroporous zeolitic imidazolate framework-8 (magMZIF-8) is synthesized used for efficient urinary isolation. Notably, beyond retaining single crystals micropores conventional ZIF-8, MZIF-8 further enhanced with highly oriented macropores (150 nm) large specific surface area (973 m

Language: Английский

Citations

5
MXene-mediated fluorescent recombinase polymerase amplification for rapid and sensitive DNA methylation biosensing DOI

Bin Guo,

Jinghua Peng, Yongzhi Wang

et al.

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127685 - 127685

Published: Feb. 3, 2025

Language: Английский

Citations

0
Advances in Nanomaterials based Laser Desorption/Ionization Mass Spectrometry for Metabolic Analysis DOI
Chenjie Yang, Shuangshuang Ji, Shun Shen

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118190 - 118190

Published: Feb. 1, 2025

Language: Английский

Citations

0
Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics DOI Creative Commons

Tomoaki Hara,

Sikun Meng, Aya Hasan Alshammari

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(5), P. 628 - 628

Published: March 5, 2025

Cancer cells exhibit abnormal behavior compared to normal cells. They ignore growth arrest signals such as contact inhibition, a mechanism that stops their proliferation when they collide with surrounding cells, and proliferate in an uncontrolled manner, destroying tissue. Early detection treatment of cancer are therefore important for healthy longevity. differ from characteristic gene expression due abnormalities. markers reflect these characteristics have been searched applied diagnosis. Although analysis blood antigens has the main method, further development diagnostic system is needed early cancer. Next-generation sequencers improved technology, making it possible analyze detailed nucleic acid molecules or urine. In addition, release extracellular vesicles, exosomes, which known contain may serve markers. This review summarizes latest findings on exosomal

Language: Английский

Citations

0