Current trends in smart mesoporous silica-based nanovehicles for photoactivated cancer therapy

Journal of Controlled Release, Journal Year: 2021, Volume and Issue: 339, P. 445 - 472

Published: Oct. 11, 2021

Language: Английский

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Molecular Engineering of pH-Responsive NIR Oxazine Assemblies for Evoking Tumor Ferroptosis via Triggering Lysosomal Dysfunction

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(6), P. 3736 - 3747

Published: Feb. 2, 2023

Ferroptosis, a newly discovered form of regulated cell death, is emerging as promising approach to tumor therapy. However, the spatiotemporal control cell-intrinsic Fenton chemistry modulate ferroptosis remains challenging. Here, we report an oxazine-based activatable molecular assembly (PTO-Biotin Nps), which capable triggering lysosomal dysfunction-mediated pathway with excellent resolution via near-infrared (NIR) light evoke ferroptosis. In this system, pH-responsive NIR photothermal oxazine molecule was designed and functionalized tumor-targeting hydrophilic biotin-poly(ethylene glycol) (PEG) chain engineer well-defined nanostructured assemblies within single-molecular framework. PTO-Biotin Nps possesses selective tropism lysosome accumulation inside cells, accommodated by its enhanced activity in acidic microenvironment. Upon activation, promoted dysfunction induced cytosolic acidification impaired autophagy. More importantly, photoactivation-mediated found markedly enhance cellular reactions ferroptosis, thereby improving antitumor efficacy mitigating systemic side effects. Overall, our study demonstrates that engineering enables modulation intrinsic mechanism, offering novel strategy for development metal-free inducers

Language: Английский

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Progress and Perspective of Solid-State Organic Fluorophores for Biomedical Applications

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(50), P. 21143 - 21160

Published: Dec. 8, 2021

Fluorescent organic dyes have been extensively used as raw materials for the development of versatile imaging tools in field biomedicine. Particularly, solid-state fluorophores (SSOFs) past 20 years has exhibited an upward trend. In recent years, studies on SSOFs focused advanced tools, such optical contrast agents and phototherapy agents, biomedical applications. However, practical application these hindered owing to several limitations. Thus, this Perspective, we provided insights that could aid researchers further develop overcome limitations limited aqueous dispersibility, low biocompatibility, uncontrolled emission. First, described inherent photophysical properties fluorescence mechanisms conventional, aggregation-induced emissive, precipitating with respect their Subsequently, highlighted functionalized bioimaging, biosensing, theranostics. Finally, elucidated potential prospects current SSOF-based associated

Language: Английский

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Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near-Infrared Bioimaging

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(31), P. 14351 - 14362

Published: July 29, 2022

Near-infrared (NIR) fluorophores absorbing maximally in the region beyond 800 nm, i.e., deep-NIR spectral region, are actively sought for biomedical applications. Ideal dyes bright, nontoxic, photostable, biocompatible, and easily derivatized to introduce functionalities (e.g., bioconjugation or aqueous solubility). The rational design of such remains a major challenge. Silicon-substituted rhodamines have been successful bioimaging applications red region. longer-wavelength silicon-substituted congeners unknown date. We successfully prepared four bis-benzannulated rhodamine (ESi5a–ESi5d), with an efficient five-step cascade on gram-scale. Because extensive overlapping their HOMO–LUMO orbitals, ESi5a–ESi5d highly (λabs ≈ 865 nm ε > 105 cm–1 M–1). By restraining both rotational freedom via annulation vibrational silicon-imparted strain, fluorochromic scaffold ESi5 is rigid, resulting unusually long fluorescence lifetime (τ 700 ps CH2Cl2) high quantum yield (ϕ = 0.14 CH2Cl2). Their half-lives toward photobleaching 2 orders magnitude longer than current standard (ICG serum). They stable presence biorelevant concentration nucleophiles reactive oxygen species. minimally toxic readily metabolized. Upon tail vein injection ESi5a (as example), vasculature nude mouse was imaged signal-to-background ratio. broad potentials

Language: Английский

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NIRII‐HDs: A Versatile Platform for Developing Activatable NIR‐II Fluorogenic Probes for Reliable In Vivo Analyte Sensing

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(19)

Published: Feb. 26, 2022

Small-molecule-based second near-infrared (NIR-II) activatable fluorescent probes can potentially provide a high target-to-background ratio and deep tissue penetration. However, most of the reported NIR-II small-molecule exhibit poor versatility owing to lack general stable optically tunable group. In this study, we designed NIRII-HDs, novel dye scaffold optimized for probe development. particular, NIRII-HD5 showed best optical properties such as proper pKa value, excellent stability, brightness, which be beneficial in vivo imaging with contrast. To demonstrate applicability dye, three target-activatable ROS, thiols, enzymes. Using these probes, not only realized reliable different diseases mouse models but also evaluated redox potential liver during injury fidelity.

Language: Английский

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