“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging DOI
Gangwei Jiang, Hong Liu,

Guohui Deng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Feb. 27, 2024

Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re-engineered far-red NIR II rhodol derivatives that possess weak BCAF interference. And a series "zero" PIF sensing-platforms were created by systematically regulating open-loop/spirocyclic forms. Leveraging advancements, devised various ultra-sensitive indicators, achieving substantial boosts (190 1300-fold). Among 8-LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum-targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Language: Английский

Recent progress in the development of fluorescent probes for imaging pathological oxidative stress DOI Creative Commons

Yujie Geng,

Zhuo Wang,

Jiaying Zhou

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(11), P. 3873 - 3926

Published: Jan. 1, 2023

Oxidative stress is closely related to the physiopathology of numerous diseases. Reactive oxygen species (ROS), reactive nitrogen (RNS), and sulfur (RSS) are direct participants important biomarkers oxidative stress.

Language: Английский

Citations

170
In vivo NIR-II fluorescence imaging for biology and medicine DOI
Feifei Wang, Yeteng Zhong, Oliver T. Bruns†

et al.

Nature Photonics, Journal Year: 2024, Volume and Issue: 18(6), P. 535 - 547

Published: March 4, 2024

Language: Английский

Citations

153
Near infrared fluorescent probes for detecting and imaging active small molecules DOI

Caiping Ding,

Tian‐Bing Ren

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 482, P. 215080 - 215080

Published: Feb. 21, 2023

Language: Английский

Citations

127
Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region DOI Creative Commons

Benhao Li,

Mengyao Zhao, Jing Lin

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(18), P. 7692 - 7714

Published: Jan. 1, 2022

This paper reviews organic and inorganic species as building blocks various design strategies of NIR-II nanohybrids for intensity-based lifetime-based bioimaging biosensing.

Language: Английский

Citations

87
Engineering of Reversible NIR‐II Redox‐Responsive Fluorescent Probes for Imaging of Inflammation In Vivo DOI
Long He,

Linhui He,

Shuai Xu

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(46)

Published: Sept. 23, 2022

The second near-infrared (NIR-II) fluorescent imaging shows great potential for deep tissue analysis at high resolution in living body owing to low background autofluorescence and photon scattering. However, reversible monitoring of redox homeostasis using NIR-II remains a challenge due the lack appropriate probes. In this study, series stable multifunctional dyes (NIR-II Cy3s) were constructed based on trimethine skeleton. Significantly, introducing 1,4-diethyl-decahydroquinoxaline group Cy3s not only effectively increased wavelength, but also served as an effective response site HClO, which can be restored by reactive sulfur species (RSS). Based this, used HClO/RSS-mediated processes pathophysiology environment. Finally, Cy3-988 was successfully utilized assessment environments drug treatment effects acute inflammation model.

Language: Английский

Citations

83
The pursuit of polymethine fluorophores with NIR-II emission and high brightness forin vivoapplications DOI Creative Commons
Xuan Zhao, Fan Zhang, Zuhai Lei

et al.

Chemical Science, Journal Year: 2022, Volume and Issue: 13(38), P. 11280 - 11293

Published: Jan. 1, 2022

Polymethine cyanine dyes, as the most important class of organic near-infrared-II (NIR-II) fluorophores, recently received increasing attention due to their high molar extinction coefficients, intensive fluorescence brightness, and flexible wavelength tunability for fluorescent bioimaging applications. Very recently, remarkable advances have been made in development NIR-II polymethine fluorophores with improved optical performance, mainly including tunable fluorescence, water solubility stability. In this review, we summarize recent research molecular tailoring design strategies then emphasize representative biosensing The potential challenges perspectives emerging field are also discussed. This review may provide guidance reference further high-performance boost clinical translation future.

Language: Английский

Citations

80
Polymethine Molecular Platform for Ratiometric Fluorescent Probes in the Second near-Infrared Window DOI
Qingchun Lan, Peng Yu,

Kui Yan

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(46), P. 21010 - 21015

Published: Oct. 25, 2022

Visualizing biomolecules such as enzymes in the deep tissue of living organisms via molecular ratiometric fluorescent probes second near-infrared window (NIR-II) with a built-in self-calibration function can provide reliable information about relevant pathophysiological processes directly but so far is not feasible due to lack fluorescence modulation strategy NIR-II window. Here we present platform Py-2 by integrating rhodamine 6G scaffold and polymethine. The maximal emission wavelength was 1010 nm blue-shifted 945 when its secondary amine acylated. Based on Py-2, two probes, nitroreductase-responsive Rap-N ROS-responsive Rap-R, were constructed successfully demonstrated vitro vivo. Overall, this report presents unique approach developing for vivo biosensing.

Language: Английский

Citations

75
Viscosity-responsive NIR-II fluorescent probe with aggregation-induced emission features for early diagnosis of liver injury DOI

Jinyin Ge,

Wenwen Cai, Niu Niu

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 300, P. 122190 - 122190

Published: June 8, 2023

Language: Английский

Citations

51
Chemical Approaches to Optimize the Properties of Organic Fluorophores for Imaging and Sensing DOI
Gangwei Jiang, Han Liu, Hong Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(11)

Published: Dec. 12, 2023

Abstract Organic fluorophores are indispensable tools in cells, tissue and vivo imaging, have enabled much progress the wide range of biological biomedical fields. However, many available dyes suffer from insufficient performances, such as short absorption emission wavelength, low brightness, poor stability, small Stokes shift, unsuitable permeability, restricting their application advanced imaging technology complex imaging. Over past two decades, efforts been made to improve these performances fluorophores. Starting with luminescence principle fluorophores, this review clarifies mechanisms performance for traditional a certain extent, systematically summarizes modified approaches optimizing properties, highlights typical applications improved sensing, indicates existing problems challenges area. This not only proves significance improving but also provide theoretical guidance development high‐performance

Language: Английский

Citations

51
Rational Design of NIR-II G-Quadruplex Fluorescent Probes for Accurate In Vivo Tumor Metastasis Imaging DOI

Ren-Xuan Wang,

Yifeng Ou,

Yushi Chen

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(17), P. 11669 - 11678

Published: April 22, 2024

Accurate in vivo imaging of G-quadruplexes (G4) is critical for understanding the emergence and progression G4-associated diseases like cancer. However, existing G4 fluorescent probes primarily operate within near-infrared region (NIR-I), which limits their application accuracy due to short emission wavelength. The transition second (NIR-II) has been significant interest, as it offers reduced autofluorescence deeper tissue penetration, thereby facilitating more accurate imaging. Nonetheless, advancement NIR-II impeded by absence effective probe design strategies. Herein, through a "step-by-step" rational approach, we have successfully developed NIRG-2, first small-molecule with tailored detection. Molecular docking calculations reveal that NIRG-2 forms stable hydrogen bonds strong π–π interactions structures, effectively inhibit twisted intramolecular charge transfer (TICT) and, thereby, selectively illuminate structures. Due its (940 nm), large Stokes shift (90 high selectivity, up 47-fold fluorescence enhancement depth 5 mm detection, significantly outperforming probes. Utilizing have, time, achieved high-contrast visualization tumor metastasis lymph nodes precise resection. Furthermore, proves be highly reliable evaluating surgical drug treatment efficacy cancer lymphatic models. We are optimistic this study not only provides crucial molecular tool an in-depth G4-related but also marks promising strategy development clinical G4-activated

Language: Английский

Citations

35