Shedding Light on Lysosomal Malondialdehyde Affecting Vitamin B12 Transport during Cerebral Ischemia/Reperfusion Injury DOI
Di Su, Ran Zhang, Xin Wang

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(41), P. 22609 - 22619

Published: Oct. 7, 2023

Cerebral ischemia-reperfusion injury (CIRI) is often accompanied by upregulation of homocysteine (Hcy). Excessive Hcy damages cerebral vascular endothelial cells and neurons, inducing neurotoxicity even neurodegeneration. Normally, supplementation vitamin B12 an ideal intervention to reduce Hcy. However, therapy clinically inefficacious for CIRI. Considering oxidative stress closely related CIRI, the lysosome pivotal site transport. Lysosomal might hinder transport B12. Whether lysosomal malondialdehyde (lysosomal MDA), as authoritative biomarker stress, interferes with has not been elucidated. This ascribed absence effective methods real-time in situ measurement MDA within living brains. Herein, a fluorescence imaging agent, Lyso-MCBH, was constructed specifically monitor entering brain targeting lysosome. Erupting level brains mice under CIRI first observed using Lyso-MCBH. found affect efficacy blocking from cytoplasm. More importantly, expression function transporter LMBD1 were proved be associated excessive MDA. Altogether, revealing MDA-LMBD1 axis provides cogent interpretation inefficacy which could prospective therapeutic target.

Language: Английский

Near-infrared II fluorescence imaging DOI
E. Schmidt, Zihao Ou, Erving Ximendes

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: April 4, 2024

Language: Английский

Citations

73
J‐Aggregation Strategy toward Potentiated NIR‐II Fluorescence Bioimaging of Molecular Fluorophores DOI
Xiaoming Hu,

Caijun Zhu,

Fengwei Sun

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(1)

Published: Aug. 1, 2023

Molecular fluorophores emitting in the second near-infrared (NIR-II, 1000-1700 nm) window with strong optical harvesting and high quantum yields hold great potential for vivo deep-tissue bioimaging high-resolution biosensing. Recently, J-aggregates are harnessed to engineer long-wavelength NIR-II emitters show unique superiority tumor detection, vessel mapping, surgical navigation, phototheranostics due their bathochromic-shifted bands required slip-stacked arrangement aggregation state. However, despite preliminary progress of theoretical study structure-property relationships, further paradigms remain scarce lack on aggregated fashion. In this effort, how utilize specific molecular structure form packing motifs J-type exciton coupling is emphatically elucidated. First, several regulating strategies achieve containing intermolecular interactions external conditions positively summarized deeply analyzed. Then, recent reports theranostics systematically provide a clear reference direction promoting development organic fluorophores. Eventually, prospective efforts ameliorating clinical practices outlined.

Language: Английский

Citations

68
Ultra-photostable small-molecule dyes facilitate near-infrared biophotonics DOI Creative Commons

Kui Yan,

Zhubin Hu, Peng Yu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 22, 2024

Abstract Long-wavelength, near-infrared small-molecule dyes are attractive in biophotonics. Conventionally, they rely on expanded aromatic structures for redshift, which comes at the cost of application performance such as photostability, cell permeability, and functionality. Here, we report a ground-state antiaromatic strategy showcase concise synthesis 14 cationic aminofluorene with mini (molecular weights: 299–504 Da) distinct spectra covering 700–1600 nm. Aminofluorene cell-permeable achieve rapid renal clearance via simple 44 Da carboxylation. This accelerates optical diagnostics injury by 50 min compared to existing macromolecular approaches. We develop compact molecular sensing platform vivo intracellular sensing, demonstrate versatile applications these multispectral fluorescence optoacoustic imaging. find that aromaticity reversal upon electronic excitation, indicated magnetic descriptors, not only reduces energy bandgap but also induces strong vibronic coupling, resulting ultrafast excited-state dynamics unparalleled photostability. These results support argument antiaromaticity useful design rule dye development, enabling performances essential modern

Language: Английский

Citations

36
Rational Design of NIR-II G-Quadruplex Fluorescent Probes for Accurate In Vivo Tumor Metastasis Imaging DOI

Ren-Xuan Wang,

Yifeng Ou,

Yushi Chen

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(17), P. 11669 - 11678

Published: April 22, 2024

Accurate in vivo imaging of G-quadruplexes (G4) is critical for understanding the emergence and progression G4-associated diseases like cancer. However, existing G4 fluorescent probes primarily operate within near-infrared region (NIR-I), which limits their application accuracy due to short emission wavelength. The transition second (NIR-II) has been significant interest, as it offers reduced autofluorescence deeper tissue penetration, thereby facilitating more accurate imaging. Nonetheless, advancement NIR-II impeded by absence effective probe design strategies. Herein, through a "step-by-step" rational approach, we have successfully developed NIRG-2, first small-molecule with tailored detection. Molecular docking calculations reveal that NIRG-2 forms stable hydrogen bonds strong π–π interactions structures, effectively inhibit twisted intramolecular charge transfer (TICT) and, thereby, selectively illuminate structures. Due its (940 nm), large Stokes shift (90 high selectivity, up 47-fold fluorescence enhancement depth 5 mm detection, significantly outperforming probes. Utilizing have, time, achieved high-contrast visualization tumor metastasis lymph nodes precise resection. Furthermore, proves be highly reliable evaluating surgical drug treatment efficacy cancer lymphatic models. We are optimistic this study not only provides crucial molecular tool an in-depth G4-related but also marks promising strategy development clinical G4-activated

Language: Английский

Citations

34
Biomedical applications of NIR-II organic small molecule fluorescent probes in different organs DOI
Lizhen Xu, Qian Zhang, Xin Wang

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 519, P. 216122 - 216122

Published: Aug. 6, 2024

Language: Английский

Citations

18
Molecular Design of NIR-II Polymethine Fluorophores for Bioimaging and Biosensing DOI Creative Commons

Yifeng Ou,

Tian‐Bing Ren, Lin Yuan

et al.

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(3), P. 220 - 233

Published: May 18, 2023

Organic polymethine fluorophores with emission in the NIR-II window (1000–1700 nm) are receiving enormous attention biomolecular medicine and bioimaging, attributed to high absorption coefficients, bright emission, excellent biocompatibility, molecule adjustability. Recently, researchers have devoted significant effort designing improving making notable progress fluorescence imaging performance. This review summarizes recent developments molecular engineering design mentality of probes highlights their extensive bioimaging biosensing applications. Furthermore, we elucidate potential challenges perspectives these dyes chemical biomedicine imaging, which may stimulate further development high-performance dye contrast agents for future clinical

Language: Английский

Citations

38
Optical and Photoacoustic Imaging In Vivo: Opportunities and Challenges DOI Creative Commons
Xuan Zhang, Ying Wu, Lanlan Chen

et al.

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(2), P. 99 - 109

Published: March 20, 2023

Optical and photoacoustic imaging plays an important role in biomedical applications owing to its noninvasiveness high resolution. Fluorescence emerge as powerful tools deconstruct molecular information investigate biological processes vivo. Despite great progress has been achieved chemical probe synthesis, how design probes with optimal fluorescence or performance dynamically visualize the process vivo still faces challenges. From this perspective, we will focus on advanced development of Furthermore, concerns prospects for future be demonstrated.

Language: Английский

Citations

36
Homotypic Membrane-Enhanced Blood–Brain Barrier Crossing and Glioblastoma Targeting for Precise Surgical Resection and Photothermal Therapy DOI
Hang Zhang,

Shujuan Guan,

Tianxiang Wei

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(10), P. 5930 - 5940

Published: March 3, 2023

The crossing of blood-brain barrier (BBB) is essential for glioblastoma (GBM) therapy, and homotypic targeting an effective strategy to achieve BBB crossing. In this work, GBM patient-derived tumor cell membrane (GBM-PDTCM) prepared cloak gold nanorods (AuNRs). Relying on the high homology GBM-PDTCM brain membrane, GBM-PDTCM@AuNRs realize efficient selective targeting. Meanwhile, owing functionalization Raman reporter lipophilic fluorophore, are able generate fluorescence signals at lesion, almost all can be precisely resected in 15 min by guidance dual signals, ameliorating surgical treatment advanced GBM. addition, photothermal therapy orthotopic xenograft mice accomplished intravenous injection GBM-PDTCM@AuNRs, doubling median survival time mice, which improves nonsurgical early Therefore, benefiting from membrane-enhanced targeting, all-stage treated with distinct ways, providing alternative idea brain.

Language: Английский

Citations

32
Nanoparticles Mediated the Diagnosis and Therapy of Glioblastoma: Bypass or Cross the Blood–Brain Barrier DOI
Xiaowei Song, Haisheng Qian,

Yongqiang Yu

et al.

Small, Journal Year: 2023, Volume and Issue: 19(45)

Published: July 7, 2023

Abstract Glioblastoma is one of the most aggressive central nervous system malignancies with high morbidity and mortality. Current clinical approaches, including surgical resection, radiotherapy, chemotherapy, are limited by difficulty targeting brain lesions accurately, leading to disease recurrence fatal outcomes. The lack effective treatments has prompted researchers continuously explore novel therapeutic strategies. In recent years, nanomedicine made remarkable progress expanded its application in drug delivery, providing a new treatment for tumors. Against this background, article reviews delivery systems paper, mechanism nanomaterials crossing blood‐brain barrier summarized. Furthermore, specific nanotechnology glioblastoma discussed depth.

Language: Английский

Citations

25
Biomimetic Gold Nanorods‐Manganese Porphyrins with Surface‐Enhanced Raman Scattering Effect for Photoacoustic Imaging‐Guided Photothermal/Photodynamic Therapy DOI
Huihui Liu, Cunji Gao,

Peijing Xu

et al.

Small, Journal Year: 2024, Volume and Issue: 20(42)

Published: June 20, 2024

Abstract Surface‐enhanced Raman scattering (SERS) imaging integrating photothermal and photodynamic therapy (PTT/PDT) is a promising approach for achieving accurate diagnosis effective treatment of cancers. However, most available reporters show multiple signals in the fingerprint region, which overlap with background from cellular biomolecules. Herein, 4T1 cell membrane‐enveloped gold nanorods‐manganese porphyrins system (GMCMs) designed successfully fabricated as biomimetic theranostic nanoplatform. Manganese are adsorbed on surface Au nanorods via terminal alkynyl group. Cell membrane encapsulation protects manganese falling off nanorods. The GMCMs confirm specific homologous targeting to cells good dispersibility, excellent photoacoustic (PA) properties, preferable 1 O 2 generation performance. exhibit distinct SERS silent region without endogenous biomolecule interference both vitro vivo. ions could not only quench fluorescence enhance effect but also deplete GSH increase yield. Both vivo studies demonstrate that effectively eradicate tumors through SERS/PA imaging‐guided PTT/PDT. This study provides feasible strategy augmenting effects group GSH‐depletion PTT/PDT efficacy.

Language: Английский

Citations

15