Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 6, 2024
The
uranyl
ion
(UO22+)
is
the
most
stable
form
of
uranium,
which
exhibits
high
toxicity
and
bioavailability
posing
a
severe
risk
to
human
health.
construction
ultrasensitive,
reliable,
robust
sensing
techniques
for
UO22+
detection
in
water
soil
samples
remains
challenge.
Herein,
DNA
network
biosensor
was
fabricated
using
DNAzyme
as
heavy
metal
recognition
element
double-loop
hairpin
probes
assembly
materials.
UO22+-activated
specific
cleavage
will
liberate
triggered
fragment,
can
be
utilized
launch
probe
among
Hab,
Hbc,
Hca.
Through
multiple
cyclic
cross-hybridization
reactions,
hexagonal
duplex
nanostructures
(n[Hab•Hbc•Hca])
were
formed.
This
system
generates
fluorescence
response
monitoring.
with
limit
2
pM.
also
displays
an
excellent
selectivity
robustness,
enabling
work
even
complex
accuracy
reliability.
With
advantages
enzyme-free
operation,
outstanding
specificity,
sensitivity,
our
proposed
provides
simple,
method
trace
levels
environmental
samples.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(19), P. 12941 - 12949
Published: April 30, 2024
Nucleic
acids
are
mainly
found
in
the
mitochondria
and
nuclei
of
cells.
Detecting
nucleic
mitochondrion
nucleus
cascade
mode
is
crucial
for
understanding
diverse
biological
processes.
This
study
introduces
a
novel
acid-based
fluorescent
styrene
dye
(SPP)
that
exhibits
light-driven
migration
from
to
nucleus.
By
introducing
N-arylpyridine
on
one
side
skeleton
bis(2-ethylsulfanyl-ethy)-amino
unit
other
side,
we
SPP
excellent
DNA
specificity
(16-fold,
FDNA/Ffree)
stronger
binding
force
nuclear
(−5.09
kcal/mol)
than
mitochondrial
(−2.59
kcal/mol).
initially
accumulates
then
migrates
within
10
s
under
light
irradiation.
tracking
damage
apoptotic
cells,
allows
successful
visualization
differences
between
apoptosis
ferroptosis.
Finally,
triphenylamine
segment
with
photodynamic
effects
was
incorporated
into
form
photosensitizer
(MTPA-SPP),
which
targets
photosensitization
irradiation
enhanced
cancer
cell
treatment.
innovative
molecule
light-triggered
mitochondrion-to-nucleus
ability
provides
feasible
approach
situ
identification
acids,
monitoring
subcellular
physiological
events,
efficient
therapy.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(36)
Published: June 14, 2024
Mitochondria,
one
of
the
most
important
organelles,
represent
a
crucial
subcellular
target
for
fundamental
research
and
biomedical
applications.
Despite
significant
advances
in
design
DNA
nanotechnologies
variety
bio-applications,
dearth
strategies
that
enable
mitochondria
targeting
molecular
imaging
therapy
remains
an
outstanding
challenge
this
field.
In
Minireview,
we
summarize
recent
progresses
on
emerging
application
nanotechnology
mitochondria-targeted
tumor
treatment.
We
first
highlight
engineering
mitochondria-localized
nanosensors
situ
detection
diverse
key
molecules
are
essential
to
maintain
mitochondrial
functions,
including
microRNA,
enzymes,
small
molecules,
metal
ions.
Then,
compile
developments
anti-tumor
therapy,
modularly
designed
nanodevices
delivery
therapeutic
agents,
programmed
assembly
interference.
will
place
emphasis
clarification
chemical
principles
how
nanobiotechnology
can
be
various
Finally,
remaining
challenges
future
directions
field
discussed,
hoping
inspire
further
development
advanced
toolkits
both
academic
clinical
regarding
mitochondria.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(14), P. 5560 - 5569
Published: March 26, 2024
Catalytic
DNA
circuits
are
desirable
for
sensitive
bioimaging
in
living
cells;
yet,
it
remains
a
challenge
to
monitor
these
intricate
signal
communications
because
of
the
uncontrolled
circuitry
leakage
and
insufficient
cell
selectivity.
Herein,
simple
yet
powerful
DNA-repairing
enzyme
(APE1)
activation
strategy
is
introduced
achieve
site-specific
exposure
catalytic
circuit
realizing
selectively
amplified
imaging
intracellular
microRNA
robust
evaluation
APE1-involved
drug
resistance.
Specifically,
reactants
firmly
blocked
by
recognition/cleavage
site
prevent
undesirable
off-site
leakage.
The
caged
has
no
target-sensing
activity
until
its
components
activated
via
enzyme-mediated
structural
reconstitution
finally
transduces
fluorescence
within
miRNA
stimulation.
designed
demonstrates
an
enhanced
signal-to-background
ratio
assay
as
compared
with
conventional
enables
cancer-cell-selective
miRNA.
In
addition,
shows
sensing
performance
visualizing
APE1-mediated
chemoresistance
cells,
which
anticipated
in-depth
clinical
diagnosis
chemotherapy
research.
JACS Au,
Journal Year:
2025,
Volume and Issue:
5(2), P. 550 - 570
Published: Feb. 6, 2025
Cell
surface
engineering
is
a
rapidly
advancing
field,
pivotal
for
understanding
cellular
physiology
and
driving
innovations
in
biomedical
applications.
In
this
regard,
DNA
nanotechnology
offers
unprecedented
potential
precisely
manipulating
functionalizing
cell
surfaces
by
virtue
of
its
inherent
programmability
versatile
functionalities.
Herein,
Perspective
provides
comprehensive
overview
emerging
trends
engineering,
focusing
on
key
nanostructure-based
tools,
their
roles
regulating
physiological
processes,
We
first
discuss
the
strategies
integrating
molecules
onto
surfaces,
including
attachment
oligonucleotides
higher-order
nanostructure.
Second,
we
summarize
impact
DNA-based
various
such
as
membrane
protein
degradation,
signaling
transduction,
intercellular
communication,
construction
artificial
components.
Third,
highlight
applications
DNA-engineered
targeted
therapies
cancer
inflammation,
well
capture/protection
diagnostic
detection.
Finally,
address
challenges
future
directions
nanotechnology-based
engineering.
This
aims
to
provide
valuable
insights
rational
design
contributing
development
precise
personalized
medicine.
Accounts of Chemical Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 30, 2024
ConspectusIn
recent
years,
the
controlled
assembly/disassembly
of
exogenous
chemical
components
inside
cells
has
become
an
emerging
approach
to
regulating
cell
functions.
However,
construction
dynamic
material
chemistry
systems
in
living
always
remains
highly
challenging
due
complicated
intracellular
microenvironment.
Nucleic
acid
is
a
category
biological
that
can
achieve
efficient
molecular
assembly
via
specific
base-pairing
and
perform
functions
Deoxyribonucleic
(DNA)
molecules
exhibit
superior
performance
assembly,
including
sequence
programmability,
molecule
recognition
ability,
nanostructure
predictability,
as
well
unique
traditional
synthetic
polymers
do
not
carry,
showing
great
superiority
systems.
Moreover,
technologies
DNA
synthesis
are
relatively
mature,
conjugation
with
functional
small
be
achieved
through
established
methods,
facilitating
DNA-based
materials
more
In
addition,
few
have
been
proven
show
responsiveness
toward
different
stimuli,
functioning
modules.In
this
Account,
we
summarize
our
work
nanoassemblies
from
perspective
stimulus
types
enzyme,
H
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(50), P. 49081 - 49087
Published: Dec. 4, 2024
Telomerase
activation
can
lead
to
the
escape
from
cell
senescence
and
immortalization,
playing
a
crucial
role
in
growth
proliferation
of
cancer
cells.
Therefore,
detection
telomerase
activity
is
essential
for
diagnosis
treatment.
Herein,
we
develop
novel
ultrasensitive
visually
detectable
platform.
By
incorporation
exonuclease-III
(Exo-III),
this
platform
achieves
dual
signal
amplification
rolling
circle
products.
Additionally,
colorimetric
analysis
3,3′,5,5′-tetramethylbiphenyl
(TMB)
chromogenic
reaction
system
provides
approach
with
unique
advantages
such
as
simplicity,
speediness,
sensitivity.
The
exhibits
high
sensitivity
specificity
actual
sample
testing,
which
aligns
closely
results
obtained
using
commercial
kits.
Moreover,
it
offers
ease-of-use
through
visual
determination
by
naked
eyes.
This
finding
indicates
that
our
proposed
sensing
method
performs
satisfactorily
detecting
real
biological
samples.
Henceforth,
believe
holds
great
potential
clinical
anticancer
drug
development.
