Aqueous Two‐Phase Submicron Droplets Catalyze DNA Nanostructure Assembly for Confined Fluorescent Biosensing DOI Creative Commons
Xili Duan,

Siyi Duan,

Zhaoyu Han

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Membraneless organelles (MLOs) are fundamental to cellular organization, enabling biochemical processes by concentrating biomolecules and regulating reactions within confined environments. While micrometer‐scale synthetic droplets extensively studied as models of MLOs, submicron remain largely unexplored despite their potential uniquely regulate biomolecular processes. Here, generated a polyethylene glycol (PEG)/dextran aqueous two‐phase system (ATPS) model investigate effect on DNA assembly in crowded The findings reveal that exhibit distinct advantages over microdroplets acting catalytic centers concentrate accelerate kinetics. This enhancement is driven cooperative mechanism wherein global crowding from PEG induces an excluded volume effect, while local dextran provides weak but nonspecific interactions with DNA. By exploiting both the confinement phase properties droplets, rapid sensitive assay developed for miRNA detection using fluorescent readouts. These highlight unique ability amplify processes, provide new insights into interplay between effects cellular‐like environments, present platform biomarker visualization.

Language: Английский

Towards the sustainable production of biomass-derived materials with smart functionality: A tutorial review DOI
Ruibin Wang,

Youguang Feng,

Dongqi Li

et al.

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(16), P. 9075 - 9103

Published: Jan. 1, 2024

Towards the sustainable production of biomass-derived materials with smart functionality.

Language: Английский

Citations

31
Light-Driven Mitochondrion-to-Nucleus DNA Cascade Fluorescence Imaging and Enhanced Cancer Cell Photoablation DOI

Tianping Xia,

Zhuoran Xia,

Peichen Tang

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(19), P. 12941 - 12949

Published: April 30, 2024

Nucleic acids are mainly found in the mitochondria and nuclei of cells. Detecting nucleic mitochondrion nucleus cascade mode is crucial for understanding diverse biological processes. This study introduces a novel acid-based fluorescent styrene dye (SPP) that exhibits light-driven migration from to nucleus. By introducing N-arylpyridine on one side skeleton bis(2-ethylsulfanyl-ethy)-amino unit other side, we SPP excellent DNA specificity (16-fold, FDNA/Ffree) stronger binding force nuclear (−5.09 kcal/mol) than mitochondrial (−2.59 kcal/mol). initially accumulates then migrates within 10 s under light irradiation. tracking damage apoptotic cells, allows successful visualization differences between apoptosis ferroptosis. Finally, triphenylamine segment with photodynamic effects was incorporated into form photosensitizer (MTPA-SPP), which targets photosensitization irradiation enhanced cancer cell treatment. innovative molecule light-triggered mitochondrion-to-nucleus ability provides feasible approach situ identification acids, monitoring subcellular physiological events, efficient therapy.

Language: Английский

Citations

16
DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics DOI
Fangzhi Yu, Xiang‐Fei Li,

Chuangui Sheng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(36)

Published: June 14, 2024

Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio-applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria-targeted tumor treatment. We first highlight engineering mitochondria-localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti-tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

Citations

12
Multiply Guaranteed Catalytic DNA Circuit for Cancer-Cell-Selective Imaging of miRNA and Robust Evaluation of Drug Resistance DOI
Zeyue Wang,

Yu Shang,

Yuxuan Zhu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(14), P. 5560 - 5569

Published: March 26, 2024

Catalytic DNA circuits are desirable for sensitive bioimaging in living cells; yet, it remains a challenge to monitor these intricate signal communications because of the uncontrolled circuitry leakage and insufficient cell selectivity. Herein, simple yet powerful DNA-repairing enzyme (APE1) activation strategy is introduced achieve site-specific exposure catalytic circuit realizing selectively amplified imaging intracellular microRNA robust evaluation APE1-involved drug resistance. Specifically, reactants firmly blocked by recognition/cleavage site prevent undesirable off-site leakage. The caged has no target-sensing activity until its components activated via enzyme-mediated structural reconstitution finally transduces fluorescence within miRNA stimulation. designed demonstrates an enhanced signal-to-background ratio assay as compared with conventional enables cancer-cell-selective miRNA. In addition, shows sensing performance visualizing APE1-mediated chemoresistance cells, which anticipated in-depth clinical diagnosis chemotherapy research.

Language: Английский

Citations

11
Emerging Trends in DNA Nanotechnology-Enabled Cell Surface Engineering DOI Creative Commons
Fan Xiao,

Xuechu Shen,

Wenqi Tang

et al.

JACS Au, Journal Year: 2025, Volume and Issue: 5(2), P. 550 - 570

Published: Feb. 6, 2025

Cell surface engineering is a rapidly advancing field, pivotal for understanding cellular physiology and driving innovations in biomedical applications. In this regard, DNA nanotechnology offers unprecedented potential precisely manipulating functionalizing cell surfaces by virtue of its inherent programmability versatile functionalities. Herein, Perspective provides comprehensive overview emerging trends engineering, focusing on key nanostructure-based tools, their roles regulating physiological processes, We first discuss the strategies integrating molecules onto surfaces, including attachment oligonucleotides higher-order nanostructure. Second, we summarize impact DNA-based various such as membrane protein degradation, signaling transduction, intercellular communication, construction artificial components. Third, highlight applications DNA-engineered targeted therapies cancer inflammation, well capture/protection diagnostic detection. Finally, address challenges future directions nanotechnology-based engineering. This aims to provide valuable insights rational design contributing development precise personalized medicine.

Language: Английский

Citations

1
Subcellular targeting strategies for protein and peptide delivery DOI
Hao Su, Guangyu Rong, Longjie Li

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 212, P. 115387 - 115387

Published: Sept. 1, 2024

Language: Английский

Citations

6
Sequential assembly of DNA nanoparticles inside cells enables lysosome interference and cell behavior regulation DOI
Sen Yang,

Cheng Yu,

Mingxing Liu

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 56, P. 102224 - 102224

Published: March 8, 2024

Language: Английский

Citations

5
Dynamic Chemistry of DNA-Based Nanoassemblies in Living Cells DOI
Nachuan Song, Hongjin Li, Chi Yao

et al.

Accounts of Chemical Research, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

ConspectusIn recent years, the controlled assembly/disassembly of exogenous chemical components inside cells has become an emerging approach to regulating cell functions. However, construction dynamic material chemistry systems in living always remains highly challenging due complicated intracellular microenvironment. Nucleic acid is a category biological that can achieve efficient molecular assembly via specific base-pairing and perform functions Deoxyribonucleic (DNA) molecules exhibit superior performance assembly, including sequence programmability, molecule recognition ability, nanostructure predictability, as well unique traditional synthetic polymers do not carry, showing great superiority systems. Moreover, technologies DNA synthesis are relatively mature, conjugation with functional small be achieved through established methods, facilitating DNA-based materials more In addition, few have been proven show responsiveness toward different stimuli, functioning modules.In this Account, we summarize our work nanoassemblies from perspective stimulus types enzyme, H

Language: Английский

Citations

5
A reverberating triple AND logic gate nanomachine for intracellular multi-enzyme tracking and MicroRNA detection DOI
Xu Zhu, Jun‐Jie Zhu,

Jin Wang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 498, P. 155631 - 155631

Published: Sept. 8, 2024

Language: Английский

Citations

4
Rolling Circle Amplification Integrating with Exonuclease-III-Assisted Color Reaction for Sensitive Telomerase Activity Analysis DOI Creative Commons
Xiaoya Liu, Xianxian Zhao, Jie Zhang

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(50), P. 49081 - 49087

Published: Dec. 4, 2024

Telomerase activation can lead to the escape from cell senescence and immortalization, playing a crucial role in growth proliferation of cancer cells. Therefore, detection telomerase activity is essential for diagnosis treatment. Herein, we develop novel ultrasensitive visually detectable platform. By incorporation exonuclease-III (Exo-III), this platform achieves dual signal amplification rolling circle products. Additionally, colorimetric analysis 3,3′,5,5′-tetramethylbiphenyl (TMB) chromogenic reaction system provides approach with unique advantages such as simplicity, speediness, sensitivity. The exhibits high sensitivity specificity actual sample testing, which aligns closely results obtained using commercial kits. Moreover, it offers ease-of-use through visual determination by naked eyes. This finding indicates that our proposed sensing method performs satisfactorily detecting real biological samples. Henceforth, believe holds great potential clinical anticancer drug development.

Language: Английский

Citations

4