A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice

Cell, Journal Year: 2018, Volume and Issue: 174(2), P. 481 - 496.e19

Published: July 1, 2018

Language: Английский

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A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine

Neuron, Journal Year: 2019, Volume and Issue: 102(4), P. 745 - 761.e8

Published: March 25, 2019

Language: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(24), P. 11707 - 11794

Published: Dec. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Language: Английский

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Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

Nature Methods, Journal Year: 2020, Volume and Issue: 17(11), P. 1156 - 1166

Published: Oct. 21, 2020

Language: Английский

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An optimized acetylcholine sensor for monitoring in vivo cholinergic activity

Nature Methods, Journal Year: 2020, Volume and Issue: 17(11), P. 1139 - 1146

Published: Sept. 28, 2020

Language: Английский

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Improved tools to study astrocytes

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(3), P. 121 - 138

Published: Feb. 10, 2020

Language: Английский

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A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Feb. 12, 2019

Abstract Adenosine 5′ triphosphate (ATP) is a universal intracellular energy source and an evolutionarily ancient, ubiquitous extracellular signal in diverse species. Here, we report the generation characterization of single-wavelength genetically encoded fluorescent sensors (iATPSnFRs) for imaging cytosolic ATP from insertion circularly permuted superfolder GFP into epsilon subunit F 0 1 -ATPase Bacillus PS3 . On cell surface within cytosol, iATPSnFR 1.0 responds to relevant concentrations (30 μM 3 mM) with fast increases fluorescence. iATPSnFRs can be targeted specific types sub-cellular compartments, imaged standard light microscopes, do not respond other nucleotides nucleosides, when fused red protein function as ratiometric indicators. After careful consideration their modest pH sensitivity, represent promising reagents space cells during variety settings, further application-specific refinements.

Language: Английский

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A genetically encoded sensor for measuring serotonin dynamics

Nature Neuroscience, Journal Year: 2021, Volume and Issue: 24(5), P. 746 - 752

Published: April 5, 2021

Language: Английский

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A Motivational and Neuropeptidergic Hub: Anatomical and Functional Diversity within the Nucleus Accumbens Shell

Neuron, Journal Year: 2019, Volume and Issue: 102(3), P. 529 - 552

Published: May 1, 2019

Language: Английский

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Next-generation interfaces for studying neural function

Nature Biotechnology, Journal Year: 2019, Volume and Issue: 37(9), P. 1013 - 1023

Published: Aug. 12, 2019

Language: Английский

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