The metastatic spread of breast cancer accelerates during sleep

Nature, Journal Year: 2022, Volume and Issue: 607(7917), P. 156 - 162

Published: June 22, 2022

Language: Английский

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Biology, vulnerabilities and clinical applications of circulating tumour cells

Nature reviews. Cancer, Journal Year: 2022, Volume and Issue: 23(2), P. 95 - 111

Published: Dec. 9, 2022

Language: Английский

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Detection of circulating tumor cells: opportunities and challenges

Biomarker Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: Aug. 12, 2022

Circulating tumor cells (CTCs) are that shed from a primary and travel through the bloodstream. Studying functional molecular characteristics of CTCs may provide in-depth knowledge regarding highly lethal diseases. Researchers working to design devices develop analytical methods can capture detect in whole blood cancer patients with improved sensitivity specificity. Techniques using samples utilize physical prosperity, immunoaffinity or combination above positive negative enrichment during separation. Further analysis is helpful monitoring, efficacy evaluation designing targeted treatment methods. Although many advances have been achieved detection characterization CTCs, several challenges still exist limit current use this burgeoning diagnostic approach. In review, brief summary biological presented. We focus on existing CTC potential clinical implications CTCs. also put forward our own views future development direction

Language: Английский

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Circulating tumor cells: from new biological insights to clinical practice

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 2, 2024

Abstract The primary reason for high mortality rates among cancer patients is metastasis, where tumor cells migrate through the bloodstream from original site to other parts of body. Recent advancements in technology have significantly enhanced our comprehension mechanisms behind bloodborne spread circulating (CTCs). One critical process, DNA methylation, regulates gene expression and chromosome stability, thus maintaining dynamic equilibrium Global hypomethylation locus-specific hypermethylation are examples changes methylation patterns that pivotal carcinogenesis. This comprehensive review first provides an overview various processes contribute formation CTCs, including epithelial-mesenchymal transition (EMT), immune surveillance, colonization. We then conduct in-depth analysis how modifications within CTCs impact each these stages during CTC dissemination. Furthermore, we explored potential clinical implications with cancer. By understanding epigenetic modifications, can gain insights into metastatic process identify new biomarkers early detection, prognosis, targeted therapies. aims bridge gap between basic research application, highlighting significance context metastasis offering avenues improving patient outcomes.

Language: Английский

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Functional analysis of circulating tumour cells: the KEY to understand the biology of the metastatic cascade

British Journal of Cancer, Journal Year: 2022, Volume and Issue: 127(5), P. 800 - 810

Published: April 28, 2022

Abstract Metastasis formation is the main cause of cancer-related death in patients with solid tumours. At beginning this process, cancer cells escape from primary tumour to blood circulation where they become circulating (CTCs). Only a small subgroup CTCs will survive during harsh journey and colonise distant sites. The in-depth analysis these metastasis-competent very challenging because their extremely low concentration peripheral blood. So far, only few groups managed expand vitro vivo be used as models for large-scale descriptive functional analyses CTCs. These have shown already high variability complexity metastatic cascade cancer, open new avenue development diagnostic therapeutic approaches.

Language: Английский

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Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine

Bioengineering, Journal Year: 2023, Volume and Issue: 10(7), P. 857 - 857

Published: July 19, 2023

Stem cells hold promise in regenerative medicine due to their ability proliferate and differentiate into various cell types. However, self-renewal multipotency also raise concerns about tumorigenicity during post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived tumors animal models clinical administrations. Therefore, assessment is crucial evaluating safety therapeutic products. Ideally, needs be performed rapidly, sensitively, cost-effectively, scalable. This article reviews approaches for assessing tumorigenicity, including models, soft agar culture, PCR, flow cytometry, microfluidics. Each method has its advantages limitations. The selection assay depends on specific study stage development product. Combining assays may provide a more comprehensive evaluation tumorigenicity. Future developments should focus optimization standardization microfluidics-based methods, as well integration single platform efficient

Language: Английский

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Time of day as a critical variable in biology

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: June 15, 2022

Abstract Background Circadian rhythms are important for all aspects of biology; virtually every aspect biological function varies according to time day. Although this is well known, variation across the day also often ignored in design and reporting research. For review, we analyzed top 50 cited papers 10 major domains sciences calendar year 2015. We repeated analysis 2019, hypothesizing that awarding a Nobel Prize 2017 achievements field circadian biology would highlight importance scientists many disciplines, improve time-of-day reporting. Results Our analyses these 1000 empirical papers, however, revealed most failed include sufficient temporal details when describing experimental methods few systematic differences existed between 2015 2019. Overall, only 6.1% reports included information about measures manipulations permit replication. Conclusions defining feature systems, knowing systems evaluated fundamentally information. Failing account hampers reproducibility laboratories, complicates interpretation results, reduces value data based predominantly on nocturnal animals extrapolating diurnal humans.

Language: Английский

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Learning from circadian rhythm to transform cancer prevention, prognosis, and survivorship care

Trends in cancer, Journal Year: 2023, Volume and Issue: 10(3), P. 196 - 207

Published: Nov. 23, 2023

Circadian timekeeping mechanisms and cell cycle regulation share thematic biological principles in responding to signals, repairing cellular damage, coordinating metabolism, allocating resources for optimal function. Recent studies show interactions between regulators circadian clock components, offering insights into potential cancer treatment approaches. Understanding control of metabolism informs timing therapies reduce adverse effects enhance efficacy. adaptability lifestyle factors, such as activity, sleep, nutrition sheds light on their impact cancer. Leveraging regulatory prevention care is vital, most risk stems from modifiable lifestyles. Monitoring factors aids assessment targeted interventions across the continuum.

Language: Английский

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Immunotherapy around the Clock: Impact of Infusion Timing on Stage IV Melanoma Outcomes

Cells, Journal Year: 2023, Volume and Issue: 12(16), P. 2068 - 2068

Published: Aug. 15, 2023

Although the impact of circadian timing on immunotherapy has yet to be integrated into clinical practice, chronoimmunotherapy is an emerging and promising field as oscillations are observed in immune cell numbers well expression targets, e.g., programmed death protein-1 its ligand 1. Concurrent retrospective studies suggest that morning infusions may lead higher effectiveness checkpoint inhibitors melanoma, non-small lung cancer, kidney cancer. This paper discusses results a study (2016–2022) exploring infusion outcomes all 73 patients with stage IV melanoma receiving at particular medical center. While median overall survival (OS) was 24.2 months (95% confidence interval [CI] 9.04–39.8), for follow-up 15.3 months, our show having more than 75% afternoon shorter OS (14.9 vs. 38.1 months; hazard ratio 0.45 [CI 0.23–0.86]; p < 0.01) expressive impacts subgroups: women, older patients, lower tumor burden outset immunotherapy. Our findings highlight potential benefits validation prospective translational randomized studies.

Language: Английский

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Growth signaling autonomy in circulating tumor cells aids metastatic seeding

PNAS Nexus, Journal Year: 2024, Volume and Issue: 3(2)

Published: Jan. 25, 2024

Abstract Self-sufficiency (autonomy) in growth signaling, the earliest recognized hallmark of cancer, is fueled by tumor cell's ability to “secrete-and-sense” factors (GFs); this translates into cell survival and proliferation that self-sustained autocrine/paracrine secretion. A Golgi-localized circuitry comprised two GTPase switches has recently been implicated orchestration signaling autonomy. Using breast cancer cells are either endowed or impaired (by gene editing) their assemble for autonomy, here we define transcriptome, proteome, phenome such an autonomous state, unravel its role during progression. We show autonomy associated with enhanced molecular programs stemness, proliferation, epithelial-mesenchymal plasticity. Autonomy both necessary sufficient anchorage-independent GF-restricted resistance anticancer drugs required metastatic Transcriptomic proteomic studies associated, a surprising degree specificity, epidermal factor receptor (EGFR)/ErbB signaling. Derivation expression signature revealed uniquely induced circulating (CTCs), harshest phase life when it deprived biologically available (EGF). also CTCs tracks therapeutic response prognosticates outcome. These data support multiple processes essential blood-borne dissemination human cancer.

Language: Английский

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