Cell Reports Medicine,
Journal Year:
2024,
Volume and Issue:
5(5), P. 101570 - 101570
Published: May 1, 2024
While
an
association
between
Parkinson's
disease
(PD)
and
viral
infections
has
been
recognized,
the
impact
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
on
PD
progression
remains
unclear.
Here,
we
demonstrate
that
SARS-CoV-2
infection
heightens
risk
using
human
embryonic
stem
cell
(hESC)-derived
dopaminergic
(DA)
neurons
a
angiotensin-converting
enzyme
(hACE2)
transgenic
(Tg)
mouse
model.
Our
findings
reveal
exacerbates
susceptibility
cellular
toxicity
in
DA
pre-treated
with
preformed
fibrils
(hPFFs).
Additionally,
nasally
delivered
infects
hACE2
Tg
mice,
aggravating
damage
initiated
by
hPFFs.
Mice
infected
display
persisting
neuroinflammation
even
after
virus
is
no
longer
detectable
brain.
A
comprehensive
analysis
suggests
inflammatory
response
mediated
astrocytes
microglia
could
contribute
to
increased
associated
SARS-CoV-2.
These
advance
our
understanding
potential
long-term
effects
PD.
Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: April 28, 2023
Heterogeneity
describes
the
differences
among
cancer
cells
within
and
between
tumors.
It
refers
to
describing
variations
in
morphology,
transcriptional
profiles,
metabolism,
metastatic
potential.
More
recently,
field
has
included
characterization
of
tumor
immune
microenvironment
depiction
dynamics
underlying
cellular
interactions
promoting
ecosystem
evolution.
been
found
most
tumors
representing
one
challenging
behaviors
ecosystems.
As
critical
factors
impairing
long-term
efficacy
solid
therapy,
heterogeneity
leads
resistance,
more
aggressive
metastasizing,
recurrence.
We
review
role
main
models
emerging
single-cell
spatial
genomic
technologies
our
understanding
heterogeneity,
its
contribution
lethal
outcomes,
physiological
challenges
consider
designing
therapies.
highlight
how
dynamically
evolve
because
leverage
this
unleash
recognition
through
immunotherapy.
A
multidisciplinary
approach
grounded
novel
bioinformatic
computational
tools
will
allow
reaching
integrated,
multilayered
knowledge
required
implement
personalized,
efficient
therapies
urgently
for
patients.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Aug. 21, 2024
Bone
defects
pose
significant
challenges
in
healthcare,
with
over
2
million
bone
repair
surgeries
performed
globally
each
year.
As
a
burgeoning
force
the
field
of
tissue
engineering,
3D
printing
offers
novel
solutions
to
traditional
transplantation
procedures.
However,
current
3D-printed
scaffolds
still
face
three
critical
material
selection,
methods,
cellular
self-organization
and
co-culture,
significantly
impeding
their
clinical
application.
In
this
comprehensive
review,
we
delve
into
performance
criteria
that
ideal
should
possess,
particular
focus
on
core
faced
by
technology
during
translation.
We
summarize
latest
advancements
non-traditional
materials
advanced
techniques,
emphasizing
importance
integrating
organ-like
technologies
bioprinting.
This
combined
approach
enables
more
precise
simulation
natural
structure
function.
Our
aim
writing
review
is
propose
effective
strategies
address
these
promote
translation
for
defect
treatment.
Molecular Biomedicine,
Journal Year:
2024,
Volume and Issue:
5(1)
Published: Feb. 12, 2024
Abstract
Cancer
is
associated
with
a
high
degree
of
heterogeneity,
encompassing
both
inter-
and
intra-tumor
along
considerable
variability
in
clinical
response
to
common
treatments
across
patients.
Conventional
models
for
tumor
research,
such
as
vitro
cell
cultures
vivo
animal
models,
demonstrate
significant
limitations
that
fall
short
satisfying
the
research
requisites.
Patient-derived
organoids,
which
recapitulate
structures,
specific
functions,
molecular
characteristics,
genomics
alterations
expression
profiles
primary
tumors.
They
have
been
efficaciously
implemented
illness
portrayal,
mechanism
exploration,
high-throughput
drug
screening
assessment,
discovery
innovative
therapeutic
targets
potential
compounds,
customized
treatment
regimen
cancer
In
contrast
conventional
organoids
offer
an
intuitive,
dependable,
efficient
model
by
conserving
phenotypic,
genetic
diversity,
mutational
attributes
originating
tumor.
Nevertheless,
organoid
technology
also
confronts
bottlenecks
challenges,
how
comprehensively
reflect
microenvironment,
angiogenesis,
reduce
costs,
establish
standardized
construction
processes
while
retaining
reliability.
This
review
extensively
examines
use
techniques
fundamental
precision
medicine.
It
emphasizes
importance
patient-derived
biobanks
development,
screening,
safety
evaluation,
personalized
Additionally,
it
evaluates
application
experimental
better
understand
mechanisms
The
intent
this
explicate
significance
present
new
avenues
future
research.
Environment International,
Journal Year:
2024,
Volume and Issue:
184, P. 108415 - 108415
Published: Jan. 8, 2024
An
increasing
number
of
harmful
environmental
factors
are
causing
serious
impacts
on
human
health,
and
there
is
an
urgent
need
to
accurately
identify
the
toxic
effects
mechanisms
these
factors.
However,
traditional
toxicity
test
methods
(e.g.,
animal
models
cell
lines)
often
fail
provide
accurate
results.
Fortunately,
organoids
differentiated
from
stem
cells
can
more
accurately,
sensitively
specifically
reflect
body.
They
also
suitable
for
specific
studies
frequently
used
in
toxicology
nowadays.
As
a
combination
organ-on-a-chip
technology,
organoids-on-a-chip
has
great
potential
toxicology.
It
controllable
physicochemical
microenvironment
not
easy
be
contaminated.
higher
homogeneity
size
shape
organoids.
In
addition,
it
achieve
vascularization
exchange
nutrients
metabolic
wastes
time.
Multi-organoids-chip
simulate
interactions
different
organs.
These
advantages
facilitate
better
function
maturity
organoids,
which
make
up
shortcomings
common
certain
extent.
This
review
firstly
discussed
limitations
testing
platforms,
leading
introduction
new
platforms:
organoids-on-a-chip.
Next,
applications
were
summarized
prospected.
Since
platforms
have
been
sufficiently
considered
previous
literature,
we
particularly
emphasized
them.
Finally,
this
opportunities
challenges
faced
by
organoids-on-a-chip,
with
expectation
that
readers
will
gain
deeper
understanding
their
value
field
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(14), P. 11427 - 11427
Published: July 13, 2023
An
organoid
is
a
3D
organization
of
cells
that
can
recapitulate
some
the
structure
and
function
native
tissue.
Recent
work
has
seen
organoids
gain
prominence
as
valuable
model
for
studying
tissue
development,
drug
discovery,
potential
clinical
applications.
The
requirements
successful
culture
in
vitro
differ
significantly
from
those
traditional
monolayer
cell
cultures.
generation
maturation
high-fidelity
entails
developing
optimizing
environmental
conditions
to
provide
optimal
cues
growth
maturation,
such
oxygenation,
mechanical
fluidic
activation,
nutrition
gradients,
etc.
To
this
end,
we
discuss
four
main
categories
bioreactors
used
culture:
stirred
(SBR),
microfluidic
(MFB),
rotating
wall
vessels
(RWV),
electrically
stimulating
(ES)
bioreactors.
We
aim
lay
out
state-of-the-art
both
commercial
in-house
developed
bioreactor
systems,
their
benefits
derived
various
tissues,
limitations
technology,
including
sterilization,
accessibility,
suitability
ease
use
long-term
culture.
Finally,
future
directions
improvements
existing
technology
how
they
may
be
enhance
specific
Neural Regeneration Research,
Journal Year:
2023,
Volume and Issue:
0(0), P. 0 - 0
Published: Jan. 1, 2023
At
the
level
of
in
vitro
drug
screening,
development
a
phenotypic
analysis
system
with
high-content
screening
at
core
provides
strong
platform
to
support
high-throughput
screening.
There
are
few
systematic
reports
on
brain
organoids,
as
new
three-dimensional
model,
terms
model
stability,
key
fingerprint,
and
schemes,
particularly
regarding
strategies
for
massive
numbers
traditional
Chinese
medicine
monomers.
This
paper
reviews
organoids
advantages
over
induced
neurons
or
cells
simulated
diseases.
The
also
highlights
prospects
from
induction
criteria
schemes
based
characteristics
application
system.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(15), P. 12528 - 12528
Published: Aug. 7, 2023
Brain
organoids
are
three-dimensional
(3D)
structures
derived
from
human
pluripotent
stem
cells
(hPSCs)
that
reflect
early
brain
organization.
These
contain
different
cell
types,
including
neurons
and
glia,
similar
to
those
found
in
the
brain.
Human
provide
unique
opportunities
model
features
of
development
not
well-reflected
animal
models.
Compared
with
traditional
cultures
models,
offer
a
more
accurate
representation
function,
rendering
them
suitable
models
for
neurodevelopmental
diseases.
In
particular,
patients’
have
enabled
researchers
study
diseases
at
stages
gain
better
understanding
disease
mechanisms.
Multi-brain
regional
assembloids
allow
investigation
interactions
between
distinct
regions
while
achieving
higher
level
consistency
molecular
functional
characterization.
Although
possess
promising
features,
their
usefulness
is
limited
by
several
unresolved
constraints,
cellular
stress,
hypoxia,
necrosis,
lack
high-fidelity
maturation,
circuit
formation.
this
review,
we
discuss
studies
overcome
natural
limitations
organoids,
emphasizing
importance
combinations
all
neural
such
as
glia
(astrocyte,
oligodendrocytes,
microglia)
vascular
cells.
Additionally,
considering
similarity
developing
brain,
regionally
patterned
organoid-derived
(NSCs)
could
serve
scalable
source
replacement
therapy.
We
highlight
potential
application
therapy
within
field.
Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
13(5)
Published: Dec. 12, 2023
Bone
defects
stemming
from
tumorous
growths,
traumatic
events,
and
diverse
conditions
present
a
profound
conundrum
in
clinical
practice
research.
While
bone
has
the
inherent
ability
to
regenerate,
substantial
anomalies
require
regeneration
techniques.
organoids
represent
new
concept
this
field,
involving
3D
self-assembly
of
bone-associated
stem
cells
guided
vitro
with
or
without
extracellular
matrix
material,
resulting
tissue
that
mimics
structural,
functional,
genetic
properties
native
tissue.
Within
scientific
panorama,
ascend
an
esteemed
status,
securing
significant
experimental
endorsement.
Through
synthesis
current
literature
pioneering
studies,
review
offers
comprehensive
survey
organoid
paradigm,
delves
into
quintessential
architecture
ontogeny
bone,
highlights
latest
progress
fabrication.
Further,
existing
challenges
prospective
directions
for
future
research
are
identified,
advocating
interdisciplinary
collaboration
fully
harness
potential
burgeoning
domain.
Conclusively,
as
technology
continues
mature,
its
implications
both
landscapes
poised
be
profound.
Bioengineering,
Journal Year:
2023,
Volume and Issue:
10(5), P. 606 - 606
Published: May 18, 2023
In
the
world
of
clinic
treatments,
3D-printed
tissue
constructs
have
emerged
as
a
less
invasive
treatment
method
for
various
ailments.
Printing
processes,
scaffold
and
free
materials,
cells
used,
imaging
analysis
are
all
factors
that
must
be
observed
in
order
to
develop
successful
3D
clinical
applications.
However,
current
research
bioprinting
model
development
lacks
diverse
methods
vascularization
result
issues
with
scaling,
size,
variations
printing
method.
This
study
analyzes
printing,
bioinks
techniques
vascularization.
These
discussed
evaluated
determine
most
optimal
strategies
Integrating
stem
endothelial
prints,
selecting
type
bioink
according
its
physical
properties,
choosing
properties
desired
printed
steps
will
aid
bioprinted