Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting DOI Open Access

Salvatore Mancuso,

Aditya Bhalerao, Luca Cucullo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11000 - 11000

Published: Oct. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Language: Английский

Bioreactors: Applications and Innovations for a Sustainable and Healthy Future—A Critical Review DOI Creative Commons
Fernanda Palladino, Paulo Ricardo Franco Marcelino, Andersen Escobar Schlogl

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(20), P. 9346 - 9346

Published: Oct. 14, 2024

Biotechnological processes are essential for developing economies that aim to stand out in future markets. The use of bioreactors is one the most important unit operations biotechnological processes, and real-time monitoring ensure precise bioprocess control. This review presents different types bioreactors, sensors, applications other sectors. Bioreactors, controlled systems cultivating microorganisms cells, tools various fields, from scientific research industrial production. a variety sensors critical accurate, monitoring, early problem detection, reproducibility, cost reduction, increased efficiency. These benefits being realized numerous applications, including biofuel production, bioremediation leaching tissue engineering, drug manufacturing. Innovations bioreactor technology expanding opportunities more sustainable healthier future. By new integrating advanced exploring promising playing key role addressing global challenges sustainably advancing science technology.

Language: Английский

Citations

11
Organoids in Haematologic Research: Advances and Future Directions DOI Creative Commons
Liangzheng Chang, Lu Li, Yuling Han

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

ABSTRACT Organoid technology, as a revolutionary biomedical tool, has shown immense potential in haematological research recent years. By using three‐dimensional (3D) cell culture systems constructed from pluripotent stem cells (PSCs) or adult (ASCs), organoids can highly mimic the characteristics of vivo organs, thereby offering significant for investigating human organ development, disease processes and treatment strategies. This review introduces development focuses on their progress research, including haematopoietic‐related organoids, immune‐related used studying blood system diseases. It discusses prospects, challenges future outlook field haematology. aims to provide latest advancements directions organoid technology references insights into further exploration this field.

Language: Английский

Citations

1
Recent progress on the organoids: Techniques, advantages and applications DOI
Xiaofeng Liu, Zhiyuan Zhou, Yao Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 185, P. 117942 - 117942

Published: March 4, 2025

Language: Английский

Citations

1
Shaping the Neurovascular Unit Exploiting Human Brain Organoids DOI Creative Commons
Mafalda Rizzuti, Valentina Melzi,

Lorenzo Brambilla

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(9), P. 6642 - 6657

Published: Feb. 9, 2024

Abstract Brain organoids, three-dimensional cell structures derived from pluripotent stem cells, closely mimic key aspects of the human brain in vitro , providing a powerful tool for studying neurodevelopment and disease. The neuroectodermal induction protocol employed organoid generation primarily gives rise to neural cellular component but lacks vital vascular system, which is crucial functions by regulating differentiation, migration, circuit formation, as well delivering oxygen nutrients. Many neurological diseases are caused dysfunctions cerebral microcirculation, making vascularization organoids an important pathogenetic translational research. Experimentally, creation vascularized has focused on fusion transplantation vivo use microfluidic devices replicate intricate microenvironment . This review summarizes these efforts highlights importance neurovascular unit forward-looking perspective leveraging their understanding treating disorders. Graphical

Language: Английский

Citations

8
A review: analysis of technical challenges in cultured meat production and its commercialization DOI
Yanyan Zheng, Zenan Hu, Guanghong Zhou

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Feb. 22, 2024

The cultured meat technology has developed rapidly in recent years, but there are still many technical challenges that hinder the large-scale production and commercialization of meat. Firstly, it is necessary to lay foundation for by obtaining seed cells maintaining stable cell functions. Next, technologies such as bioreactors used expand scale culture, three-dimensional culture scaffold or 3D printing construct structure At same time, can reduce costs developing serum-free medium suitable Finally, edible quality improved evaluating food safety sensory flavor, combining ethical consumer acceptability issues. Therefore, this review fully demonstrates current development status existing with regard key points described above, order provide research ideas industrial

Language: Английский

Citations

8
Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine DOI Creative Commons
Qigu Yao, Sheng Cheng, Qiaoling Pan

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 21, 2024

Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions specific organs. Although field organoids has experienced exponential growth, driven by advances in artificial intelligence, gene editing, bioinstrumentation, a comprehensive overview organoid applications remains necessary. This review offers detailed exploration historical origins characteristics various types, their applications-including disease modeling, drug toxicity efficacy assessments, precision medicine, regenerative medicine-as well as current challenges future directions research. have proven instrumental elucidating genetic cell fate hereditary diseases, infectious metabolic disorders, malignancies, study processes such embryonic development, molecular mechanisms, host-microbe interactions. Furthermore, integration technology with intelligence microfluidics significantly advanced large-scale, rapid, cost-effective thereby propelling progress medicine. Finally, advent high-performance materials, three-dimensional printing technology, also gaining prominence Our insights predictions aim to provide valuable guidance researchers support continued advancement this rapidly developing field.

Language: Английский

Citations

8
Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research DOI Open Access
Chawon Yun, Sou Hyun Kim,

Kyung Mok Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2512 - 2512

Published: Feb. 21, 2024

Bone differentiation is crucial for skeletal development and maintenance. Its dysfunction can cause various pathological conditions such as rickets, osteoporosis, osteogenesis imperfecta, or Paget's disease. Although traditional two-dimensional cell culture systems have contributed significantly to our understanding of bone biology, they fail replicate the intricate biotic environment tissue. Three-dimensional (3D) spheroid cultures gained widespread popularity addressing defects. This review highlights advantages employing 3D investigate differentiation. It their capacity mimic complex in vivo cellular interactions pivotal homeostasis. The exploration models research offers enhanced physiological relevance, improved predictive capabilities, reduced reliance on animal models, which advancement safer more effective strategies drug development. Studies highlighted transformative potential expanding biology developing targeted therapeutic interventions bone-related disorders. explores how demonstrated promise unraveling mechanisms governing homeostasis responses pharmacological agents.

Language: Английский

Citations

7
Toward reproducible tumor organoid culture: focusing on primary liver cancer DOI Creative Commons

Lianming Guo,

Chao Li,

Weiqiang Gong

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

Organoids present substantial potential for pushing forward preclinical research and personalized medicine by accurately recapitulating tissue tumor heterogeneity in vitro . However, the lack of standardized protocols cancer organoid culture has hindered reproducibility. This paper comprehensively reviews current challenges associated with highlights recent multidisciplinary advancements field a specific focus on standardizing liver culture. We discuss non-standardized aspects, including sources, processing techniques, medium formulations, matrix materials, that contribute to technical variability. Furthermore, we emphasize need establish reproducible platforms preserve genetic, proteomic, morphological, pharmacotypic features parent tumor. At end each section, our shifts standardization primary cancer. By addressing these challenges, can enhance reproducibility clinical translation systems, enabling their applications precision medicine, drug screening, research.

Language: Английский

Citations

7
Uniform sized cancer spheroids production using hydrogel-based droplet microfluidics: a review DOI
Sung‐Jin Kim,

Po Lam,

Arul Jayaraman

et al.

Biomedical Microdevices, Journal Year: 2024, Volume and Issue: 26(2)

Published: May 29, 2024

Language: Английский

Citations

5
Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions DOI Open Access
Fatima Abdelfattah, Herbert Schulz, Markus Wehland

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10014 - 10014

Published: Sept. 17, 2024

The primary objective of omics in space with focus on the human organism is to characterize and quantify biological factors that alter structure, morphology, function, dynamics cells exposed microgravity. This review discusses exciting data regarding genomics, transcriptomics, epigenomics, metabolomics, proteomics individuals space, as well cultured under simulated NASA Twins Study significantly heightened interest applying technologies bioinformatics terrestrial environments. Here, we present available publications this field a specialized stem real microgravity conditions. We summarize current knowledge following topics: (i) studies cells, (ii) benign different cell types organism, (iii) discussing advantages for commercialization exploration, (iv) summarizing emerging opportunities translational regenerative medicine travelers patients Earth.

Language: Английский

Citations

5