Selenium and Selenoproteins in Health

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 799 - 799

Published: May 8, 2023

Selenium is a trace mineral that essential for health. After being obtained from food and taken up by the liver, selenium performs various physiological functions in body form of selenoproteins, which are best known their redox activity anti-inflammatory properties. stimulates activation immune cells important system. also maintenance brain function. supplements can regulate lipid metabolism, cell apoptosis, autophagy, have displayed significant alleviating effects most cardiovascular diseases. However, effect increased intake on risk cancer remains unclear. Elevated serum levels associated with an type 2 diabetes, this relationship complex nonlinear. supplementation seems beneficial to some extent; however, existing studies not fully explained influence Further, more intervention trials needed verify or harmful

Language: Английский

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At the crossroads of immunotherapy for oncogene-addicted subsets of NSCLC

Nature Reviews Clinical Oncology, Journal Year: 2023, Volume and Issue: 20(3), P. 143 - 159

Published: Jan. 13, 2023

Language: Английский

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Advances in single-cell RNA sequencing and its applications in cancer research

Journal of Hematology & Oncology, Journal Year: 2023, Volume and Issue: 16(1)

Published: Aug. 24, 2023

Abstract Cancers are a group of heterogeneous diseases characterized by the acquisition functional capabilities during transition from normal to neoplastic state. Powerful experimental and computational tools can be applied elucidate mechanisms occurrence, progression, metastasis, drug resistance; however, challenges remain. Bulk RNA sequencing techniques only reflect average gene expression in sample, making it difficult understand tumor heterogeneity microenvironment. The emergence development single-cell (scRNA-seq) technologies have provided opportunities subtle changes biology identifying distinct cell subpopulations, dissecting microenvironment, characterizing cellular genomic mutations. Recently, scRNA-seq technology has been increasingly used cancer studies explore which increased understanding tumorigenesis evolution. This review summarizes basic processes their increasing applications research clinical practice.

Language: Английский

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A novel signature predicts prognosis and immunotherapy in lung adenocarcinoma based on cancer-associated fibroblasts

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 31, 2023

Background Extensive research has established the significant correlations between cancer-associated fibroblasts (CAFs) and various stages of cancer development, including initiation, angiogenesis, progression, resistance to therapy. In this study, we aimed investigate characteristics CAFs in lung adenocarcinoma (LUAD) develop a risk signature predict prognosis patients with LUAD. Methods We obtained single-cell RNA sequencing (scRNA-seq) bulk RNA-seq data from public database. The Seurat R package was used process scRNA-seq identify CAF clusters based on several biomarkers. CAF-related prognostic genes were further identified using univariate Cox regression analysis. To reduce number genes, Lasso performed, established. A novel nomogram that incorporated clinicopathological features developed clinical applicability model. Additionally, conducted immune landscape immunotherapy responsiveness analyses. Finally, performed vitro experiments verify functions EXO1 Results 5 LUAD data, which 3 significantly associated total 492 found be linked 1731 DEGs construct signature. Moreover, our exploration revealed related scores, its ability confirmed. Furthermore, incorporating showed excellent applicability. verified EXP1 through experiments. Conclusions proven an predictor prognosis, stratifying more appropriately precisely predicting responsiveness. comprehensive characterization can response immunotherapy, thus offering fresh perspectives into management patients. Our study ultimately confirms role facilitating invasion growth tumor cells Nevertheless, validation achieved by conducting vivo

Language: Английский

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LPCAT3 Is Transcriptionally Regulated by YAP/ZEB/EP300 and Collaborates with ACSL4 and YAP to Determine Ferroptosis Sensitivity

Antioxidants and Redox Signaling, Journal Year: 2023, Volume and Issue: 39(7-9), P. 491 - 511

Published: May 11, 2023

Aims: Lipid peroxidation occurring in lung adenocarcinoma (LUAD) cells leads to ferroptosis. Lysophosphatidylcholine acyl-transferase 3 (LPCAT3) plays a key role providing raw materials for lipid by promoting esterification of polyunsaturated fatty acids phospholipids. Whether LPCAT3 determines ferroptosis sensitivity and the mechanism which its expression is regulated LUAD has not been reported. Results: acyl-coenzyme A (CoA) synthetase long-chain family member (ACSL)4 levels were positively associated with cell lines. Overexpression ACSL4 sensitized ferroptosis, while knockout showed opposite effect. Zinc-finger E-box-binding (ZEB) was shown directly bind promoter stimulate transcription Yes-associated protein (YAP)-dependent manner. An interaction between YAP ZEB also observed. E1A-binding p300 (EP300) simultaneously bound ZEB, induced H3K27Ac transcription. This verified primary xenograft models. The ACSL4, LPCAT3, combination can jointly determine sensitivity. Innovation: binding site exists -1600 -1401 nt region promoter, promotes after binding. bind, zinc-finger cluster domain WW are crucial their EP300 may via Bromo CBP/p300-HAT domain. In addition, better than prostaglandin-endoperoxide synthase 2 (PTGS2), transferrin receptor (TFRC), or NADPH oxidase 1 (NOX1). Conclusion: YAP, EP300. be determined YAP. Antioxid. Redox Signal. 39, 491-511.

Language: Английский

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Multiplex immunofluorescence and single‐cell transcriptomic profiling reveal the spatial cell interaction networks in the non‐small cell lung cancer microenvironment

Clinical and Translational Medicine, Journal Year: 2023, Volume and Issue: 13(1)

Published: Jan. 1, 2023

Abstract Background Conventional immunohistochemistry technologies were limited by the inability to simultaneously detect multiple markers and lack of identifying spatial relationships among cells, hindering understanding biological processes in cancer immunology. Methods Tissue slices primary tumours from 553 IA∼IIIB non‐small cell lung (NSCLC) cases stained multiplex immunofluorescence (mIF) assay for 10 markers, including CD4, CD38, CD20, FOXP3, CD66b, CD8, CD68, PD‐L1, CD133 CD163, evaluating amounts 26 phenotypes cells tumour nest stroma. StarDist depth learning model was utilised determine location based on mIF graphs. Single‐cell RNA sequencing (scRNA‐seq) four NSCLC conducted investigate putative interaction networks. Results Spatial proximity CD20+ B CD4+ T CD38+ ( r 2 = 0.41) observed, whereas distribution regulatory associated with decreased infiltration levels −0.45). Univariate Cox analyses identified closer between CD8+ predicted longer disease‐free survival (DFS). In contrast, CD133+ stem (CSCs), distances neutrophils, neutrophils correlated dismal DFS. Data scRNA‐seq further showed that spatially adjacent N1‐like could boost proliferation activation lymphocytes, neighbouring M2‐like macrophages negative effects. An immune‐related risk score (IRRS) system aggregating robust quantitative prognosticators high‐IRRS patients had significantly worse DFS than low‐IRRS ones (HR 2.72, 95% CI 1.87–3.94, p < .001). Conclusions We developed a framework analyse networks microenvironment, revealing architecture intricate interplays immune cells.

Language: Английский

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Efficacy, Safety, and Biomarker Analysis of Neoadjuvant Camrelizumab and Apatinib in Patients With Resectable NSCLC: A Phase 2 Clinical Trial

Journal of Thoracic Oncology, Journal Year: 2023, Volume and Issue: 18(6), P. 780 - 791

Published: March 2, 2023

Language: Английский

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Identification and integration analysis of a novel prognostic signature associated with cuproptosis-related ferroptosis genes and relevant lncRNA regulatory axis in lung adenocarcinoma

Aging, Journal Year: 2023, Volume and Issue: unknown

Published: March 3, 2023

Lung adenocarcinoma (LUAD) is a highly prevalent malignancy worldwide, and its clinical prognosis assessment treatment major research direction. Both ferroptosis cuproptosis are novel forms of cell death considered to be important factors involved in cancer progression. To further understand the correlation between cuproptosis-related genes (CRFGs) LUAD, we explore molecular mechanisms related development disease. We constructed prognostic signature containing 13 CRFGs, which, after grouping based on risk score, revealed that LUAD high-risk group exhibited poor prognosis. Nomogram confirmed it could an independent factor for ROC curves DCA validated validity model. Further analysis showed three biomarkers (LIFR, CAV1, TFAP2A) were significantly correlated with immunization. Meanwhile, found LINC00324/miR-200c-3p/TFAP2A regulatory axis progression LUAD. In conclusion, our report reveals CRFGs well provide new ideas construction tools, immunotherapy, targeted therapy

Language: Английский

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Cytokines and soluble mediators as architects of tumor microenvironment reprogramming in cancer therapy

Cytokine & Growth Factor Reviews, Journal Year: 2024, Volume and Issue: 76, P. 12 - 21

Published: Feb. 28, 2024

Language: Английский

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Single-cell RNA sequencing of cervical exfoliated cells reveals potential biomarkers and cellular pathogenesis in cervical carcinogenesis

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(2)

Published: Feb. 12, 2024

Abstract Cervical cancer (CC) is a common gynecological malignancy. Despite the current screening methods have been proved effectively and significantly decreased CC morbidity mortality, deficiencies still exist. Single-cell RNA sequencing (scRNA-seq) approach can identify complex rare cell populations at single-cell resolution. By scRNA-seq, heterogeneity of tumor microenvironment across cervical carcinogenesis has mapped described. Whether these alterations could be detected applied to unclear. Herein, we performed scRNA-seq 56,173 exfoliated cells from 15 samples, including normal cervix, low-grade squamous intraepithelial lesion (LSIL), high-grade (HSIL), The present study delineated alteration immune epithelial derived during progression. A subset lipid-associated macrophage was identified as tumor-promoting element serve biomarker for predicting progression LSIL into HSIL, which then verified by immunofluorescence. Furthermore, cell–cell communication analysis indicated SPP1-CD44 axis might exhibit protumor interaction between macrophage. In this study, investigated multicellular ecosystem in potential biomarkers early detection.

Language: Английский

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