Novel Insights Into the Potential Mechanisms of N6-Methyladenosine RNA Modification on Sepsis-Induced Cardiovascular Dysfunction: An Update Summary on Direct and Indirect Evidences

Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9

Published: Nov. 12, 2021

Sepsis is a life-threatening organ dysfunction caused by host’s dysfunctional response to infection. As known all, septic heart disease occurs because pathogens invading the blood stimulate activation of endothelial cells, causing large number white cells accumulate and trigger an immune response. However, in severe sepsis, hematopoietic system inhibited, there will also be decline at which time autoimmune suppressed. During response, inflammatory factors are released into participate process, ultimately damages cardiac myocytes leads impaired function. N6-methyladenosine (m6A) common RNA modification mRNA non-coding that affects splicing, translation, stability, epigenetic effects some RNAs. A emerging evidences demonstrated m6A had been involved multiple biological processes, especially for sepsis disorders. Unfortunately, limited results provided analyze association between sepsis-induced cardiovascular (SICD). In this review, we firstly summarized current on how mediates pathophysiological process development cardiomyopathy emphasize importance methylation maintaining biogenesis homeostasis. Then, clarified participants extended responses activation, dominant initial changes secondary attack. After that, deeply analyzed top causes SICD identified cytokines, cell dysfunction, mitochondrial failure. Thus, highlight review systematically collected all related potential mechanisms causes. Although lack direct SICD, indirect case every particular molecular mechanism signal transduction, require further explorations links among listed mechanisms. This provides novel insights understanding SICD.

Language: Английский

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Changes in DNA methylation associated with a specific mode of delivery: a pilot study

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 18, 2024

Background The mode of delivery represents an epigenetic factor with potential to affect further development the individual by multiple mechanisms. DNA methylation may be one them, representing a major mechanism involving direct chemical modification individual’s DNA. This pilot study aims examine whether specific induces changes comparing umbilical cord blood and peripheral newborns. Methods Blood samples from infants born vaginal caesarean section were analysed prepare Methylseq library according NEBNext enzymatic Methyl-seq Methylation Library Preparation Kit generation target-enriched libraries using Twist Human Methylome Panel. status was determined Illumina next-generation sequencing (NGS). Results We identified 168 differentially methylated regions in 157 samples. These associated 59 common biological, metabolic signalling pathways for Conclusion Caesarean is likely represent important induce genome that could play role broad spectrum disorders. Our results contribute elucidation how factors, such as delivery, have adverse impact on health later their life.

Language: Английский

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Influence of DNA Methylation on Vascular Smooth Muscle Cell Phenotypic Switching

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3136 - 3136

Published: March 8, 2024

Vascular smooth muscle cells (VSMCs) are crucial components of the arterial wall, controlling blood flow and pressure by contracting relaxing artery walls. VSMCs can switch from a contractile to synthetic state, leading increased proliferation migratory potential. Epigenetic pathways, including DNA methylation, play role in regulating VSMC differentiation phenotypic flexibility. methylation involves attaching methyl group 5’ carbon cytosine base, which regulates gene expression interacting with transcription factors. Understanding key factors influencing plasticity may help identify new target molecules for development innovative drugs treat various vascular diseases. This review focuses on pathways VSMCs, summarizing mechanisms involved remodeling, significantly enhance our understanding related provide promising therapeutic approaches complex multifactorial

Language: Английский

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The Role of Selected lncRNAs in Lipid Metabolism and Cardiovascular Disease Risk

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9244 - 9244

Published: Aug. 26, 2024

Lipid disorders increase the risk for development of cardiometabolic disorders, including type 2 diabetes, atherosclerosis, and cardiovascular disease. Lipids levels, apart from diet, smoking, obesity, alcohol consumption, lack exercise, are also influenced by genetic factors. Recent studies suggested role long noncoding RNAs (lncRNAs) in regulation lipid formation metabolism. Despite their protein-coding capacity, lncRNAs crucial regulators various physiological pathological processes since they affect transcription epigenetic chromatin remodelling. LncRNAs act as molecular signal, scaffold, decoy, enhancer, guide molecules. This review summarises available data concerning impact on levels metabolism, well disease risk. relationship is significant because altered metabolism a well-known factor diseases, may play regulatory role. Understanding these mechanisms could pave way new therapeutic strategies to mitigate through targeted modulation lncRNAs. The identification dysregulated pose promising candidates interventions, enabling restoration offer an effective means impede progression without disrupting normal biological functions. serve valuable biomarker states, However, still much remains unknown about functions most lncRNAs, thus extensive necessary elucidate roles physiology, development,

Language: Английский

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Insights into RNA N6-methyladenosine and programmed cell death in atherosclerosis

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Sept. 3, 2024

Abstract N6-methyladenosine (m 6 A) modification stands out among various RNA modifications as the predominant form within eukaryotic cells, influencing numerous cellular processes implicated in disease development. m A has gained increasing attention development of atherosclerosis and become a research hotspot recent years. Programmed cell death (PCD), encompassing apoptosis, autophagy, pyroptosis, ferroptosis, necroptosis, plays pivotal role pathogenesis. In this review, we delve into intricate interplay between diverse PCD pathways, shedding light on their complex association during onset progression atherosclerosis. Clarifying relationship is great significance to provide novel strategies for cardiovascular treatment.

Language: Английский

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