Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)
Published: March 15, 2020
Directed breeding of horticultural crops is essential for increasing yield, nutritional content, and consumer-valued characteristics such as shape color the produce. However, limited genetic diversity restricts amount crop improvement that can be achieved through conventional approaches. Natural changes in
Language: Английский
GM crops & food, Journal Year: 2020, Volume and Issue: 12(1), P. 125 - 144
Published: Oct. 20, 2020
Plants are susceptible to phytopathogens, including bacteria, fungi, and viruses, which cause colossal financial shortfalls (pre- post-harvest) threaten global food safety. To combat with these plant possesses two-layer of defense in the form PAMP-triggered immunity (PTI), or Effectors-triggered (ETI). The understanding plant-molecular interactions revolution high-throughput molecular techniques have opened door for innovations developing pathogen-resistant plants. In this context, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) has transformed genome editing (GE) technology being harnessed altering traits. Here we summarized complexities immune system use CRISPR-Cas9 edit various components acquire long-lasting resistance plants against phytopathogens. This review also sheds light on limitations system, regulation edited crops future prospective technology.
Language: Английский
Citations
81
Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)
Published: July 17, 2021
Abstract Breeding has been used successfully for many years in the fruit industry, giving rise to most of today’s commercial cultivars. More recently, new molecular breeding techniques have addressed some constraints conventional breeding. However, development and introduction such novel fruits slow limited with only five genetically engineered currently produced as varieties—virus-resistant papaya squash were commercialized 25 ago, whereas insect-resistant eggplant, non-browning apple, pink-fleshed pineapple approved commercialization within last 6 production continues increase every year. Advances genetics, particularly wave genome editing technologies, provide opportunities develop cultivars more rapidly. Our review, emphasizes socioeconomic impact current developed by genetic engineering potential on improved at an accelerated rate.
Language: Английский
Citations
81
PLANT PHYSIOLOGY, Journal Year: 2021, Volume and Issue: 187(4), P. 2230 - 2245
Published: June 26, 2021
Tomato (Solanum lycopersium), an important fruit crop worldwide, requires efficient sugar allocation for development. However, molecular mechanisms import to fruits remain poorly understood. Expression of sugars will eventually be exported transporters (SWEETs) proteins is closely linked high fructose/glucose ratios in tomato and may involved allocation. Here, we discovered that SlSWEET15 highly expressed developing compared vegetative organs. In situ hybridization β-glucuronidase fusion analyses revealed accumulate vascular tissues seed coats, major sites sucrose unloading fruits. Localizing SlSWEET15-green fluorescent protein the plasma membrane supported its putative role apoplasmic unloading. The transport activity was confirmed by complementary growth assays a yeast (Saccharomyces cerevisiae) mutant. Elimination function clustered regularly interspaced short palindromic repeats (CRISPRs)/CRISPR-associated gene editing significantly decreased average sizes weights fruits, with severe defects filling embryo Altogether, our studies suggest mediating efflux from releasing phloem cells apoplasm subsequent into storage parenchyma during Furthermore, SlSWEET15-mediated likely required coat embryo.
Language: Английский
Citations
72
Plant Biotechnology Journal, Journal Year: 2021, Volume and Issue: 19(4), P. 660 - 670
Published: March 4, 2021
Summary Human population growth has increased the demand for food crops, animal feed, biofuel and biomaterials, all while climate change is impacting environmental conditions. There an urgent need to develop crop varieties which tolerate adverse conditions requiring fewer inputs. Plant breeding critical global security and, it benefited from modern technologies, remains constrained by a lack of valuable genetic diversity, linkage drag, effective way combine multiple favourable alleles complex traits. CRISPR/Cas technology transformed genome editing across biological systems promises transform agriculture with its high precision, ease design, multiplexing ability low cost. We discuss integration CRISPR/Cas‐based gene into advance domestication refine inbred various applications environments. highlight use fix desirable allelic variants, generate novel alleles, break deleterious linkages, support pre‐breeding introgression loci elite lines.
Language: Английский
Citations
60
Elsevier eBooks, Journal Year: 2021, Volume and Issue: unknown, P. 633 - 654
Published: Jan. 1, 2021
Language: Английский
Citations
57
Plant Breeding and Biotechnology, Journal Year: 2022, Volume and Issue: 10(1), P. 1 - 14
Published: March 1, 2022
Language: Английский
Citations
54
Plants, Journal Year: 2022, Volume and Issue: 11(10), P. 1297 - 1297
Published: May 12, 2022
Technological applications in agriculture have evolved substantially to increase crop yields and quality meet global food demand. Conventional techniques, such as seed saving, selective breeding, mutation breeding (variation breeding), dramatically increased production, especially during the 'Green Revolution' 1990s. However, newer issues, limited arable lands, climate change, ever-increasing demand, pose challenges agricultural production threaten security. In following 'Gene era, rapid innovations biotechnology field provide alternative strategies further improve yield, quality, resilience towards biotic abiotic stresses. These include introduction of DNA recombinant technology genome editing transcription activator-like effector (TALEN), zinc-finger nucleases (ZFN), clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas) systems. acceptance future these modern tools rely on regulatory frameworks governing their development various countries. Herein, we examine evolution technological agriculture, focusing motivations for introduction, technical challenges, possible benefits concerns, genetically engineered product production.
Language: Английский
Citations
46
Journal of Integrative Plant Biology, Journal Year: 2022, Volume and Issue: 65(2), P. 444 - 467
Published: Dec. 7, 2022
Precise replacement of an allele with elite controlling important agronomic trait in a predefined manner by gene editing technologies is highly desirable crop improvement. Base and prime are two newly developed precision systems which can introduce the substitution single base install desired short indels to target loci absence double-strand breaks donor repair templates, respectively. Since their discoveries, various strategies have been attempted optimize both editor (BE) (PE) order improve precise efficacy, specificity, expand targeting scopes. Here, we summarize latest development BEs PEs, as well applications plants. Based on these progresses, recommend appropriate PEs for basic plant research Moreover, propose perspectives further optimization editors. We envision that will become routine customized tools biological improvement near future.
Language: Английский
Citations
46
Biology, Journal Year: 2022, Volume and Issue: 11(2), P. 235 - 235
Published: Feb. 1, 2022
Tomato, one of the most cultivated and economically important vegetable crops throughout world, is affected by a panoply different pathogens that reduce yield affect product quality. The study tomato–pathogen system arises as an ideal for better understanding molecular mechanisms underlying disease resistance, offering opportunity improving quality products. Among several genes already identified in tomato response to pathogens, we highlight those encoding transcription factors (TFs). TFs act transcriptional activators or repressors gene expression are involved large-scale biological phenomena. They key regulators central components plant innate immune basal defense diverse processes, including responses pathogens. Here, present overview recent studies regarding biotic stresses. Hence, focus on families TFs, selected their abundance, importance, availability functionally well-characterized members pathogen attack. Tomato TFs’ roles possibilities related use engineering resistance presented. With this review, intend provide new insights into regulation against invading view breeding.
Language: Английский
Citations
39
Plant Cell & Environment, Journal Year: 2023, Volume and Issue: 46(12), P. 3663 - 3679
Published: Aug. 9, 2023
Abstract For many fruit crops, the colour of outwardly defines its eating quality. Fruit pigments provide reproductive advantage for plant as well providing protection against unfavourable environmental conditions and pathogens. consumers these colours are considered attractive dietary benefits derived from fruits. In majority species, main either carotenoids and/or anthocyanins. They produced in part ripening process, orchestrated by phytohormones an ensuing transcriptional cascade, culminating pigment biosynthesis. Whilst this is a controlled developmental production also attuned to such light quantity quality, availability water ambient temperature. If factors intensify stress levels, tissues respond increasing (or ceasing) production. cases, if not severe, can have positive outcome Here, we focus on principal (light, temperature water) that influence colour.
Language: Английский
Citations
24