MdMYB305–MdbHLH33–MdMYB10 regulates sugar and anthocyanin balance in red‐fleshed apple fruits

The Plant Journal, Journal Year: 2023, Volume and Issue: 113(5), P. 1062 - 1079

Published: Jan. 6, 2023

SUMMARY Sugar and anthocyanin are important indicators of fruit quality, understanding the mechanism underlying their accumulation is essential for breeding high‐quality fruit. We identified an R2R3‐MYB transcription factor MdMYB305 in red‐fleshed apple progeny, which was positively correlated with sugar content but negatively content. Transient injection, stable expression [overexpressing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)], heterologous transformation tomato confirmed that promotes inhibits synthesis anthocyanin. A series molecular experiments (such as electrophoretic mobility shift luciferase assays) combines sugar‐related genes ( MdCWI1 / MdVGT3 MdTMT2 ) anthocyanin‐related MdF3H MdDFR MdUFGT ), promoting inhibiting activities, finally regulating fruits. In addition, study also found competes MdMYB10 MdbHLH33 binding site to balance fruits, provides a reference value exploring more functions MYB‐bHLH‐MYB complex relationship between future.

Language: Английский

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Key transcription factors regulate fruit ripening and metabolite accumulation in tomato

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(3), P. 2256 - 2273

Published: April 2, 2024

Abstract Fruit ripening is a complex process involving dynamic changes to metabolites and controlled by multiple factors, including transcription factors (TFs). Several TFs are reportedly essential regulators of tomato (Solanum lycopersicum) fruit ripening. To evaluate the effects specific on metabolite accumulation during ripening, we combined CRISPR/Cas9-mediated mutagenesis with metabolome transcriptome analyses explore regulatory mechanisms. Specifically, generated various genetically engineered lines that differed regarding contents colors. The transcript profiles indicated selected have distinct functions control contents, especially carotenoids sugars. Moreover, mutation ELONGATED HYPOCOTYL5 (HY5) increased fructose glucose approximately 20% (relative wild-type levels). Our in vitro assay showed HY5 can bind directly G-box cis-element Sugars Will Eventually be Exported Transporter (SWEET12c) promoter activate expression, thereby modulating sugar transport. findings provide insights into mechanisms regulating metabolic networks, providing theoretical basis for breeding horticultural crops produce diverse flavors

Language: Английский

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Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: Aug. 1, 2021

Abstract Sugars, especially glucose and fructose, contribute to the taste quality of tomato fruits. These compounds are translocated from leaves fruits then unloaded into by various sugar transporters at plasma membrane. SWEETs, that regulate efflux independently energy or pH. To date, role SWEETs in has received very little attention. In this study, we performed functional analysis SlSWEET7a SlSWEET14 gain insight regulation transport storage were mainly expressed peduncles, vascular bundles, seeds. Both membrane-localized proteins glucose, sucrose. Apart resulting increase mature fruit content, silencing resulted taller plants larger (in -silenced lines). We also found invertase activity gene expression some SlSWEET members increased, which was consistent with increased availability sucrose hexose Overall, our results demonstrate suppressing could be a potential strategy for enhancing content

Language: Английский

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Emerging Roles of SWEET Sugar Transporters in Plant Development and Abiotic Stress Responses

Cells, Journal Year: 2022, Volume and Issue: 11(8), P. 1303 - 1303

Published: April 12, 2022

Sugars are the major source of energy in living organisms and play important roles osmotic regulation, cell signaling storage. SWEETs (Sugars Will Eventually be Exported Transporters) most recent family sugar transporters that function as uniporters, facilitating diffusion molecules across membranes. In plants, multiple physiological processes including phloem loading, senescence, pollen nutrition, grain filling, nectar secretion, abiotic (drought, heat, cold, salinity) biotic stress regulation. this review, we summarized role SWEET plant development stress. The gene expression dynamics various under stresses different species also discussed. Finally, discuss utilization genome editing tools (TALENs CRISPR/Cas9) to engineer genes can facilitate trait improvement. Overall, advancements on highlighted, which could used for crop improvement tolerance.

Language: Английский

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Contributions of sugar transporters to crop yield and fruit quality

Journal of Experimental Botany, Journal Year: 2022, Volume and Issue: 73(8), P. 2275 - 2289

Published: Feb. 4, 2022

Abstract The flux, distribution, and storage of soluble sugars regulate crop yield in terms starch, oil, protein, total carbohydrates, affect the quality many horticultural products. Sugar transporters contribute to phloem loading unloading. mechanisms have been studied detail, but complex diverse unloading sugar sink organs are less explored. Unloading subsequent transport for carbohydrates vary different organs. Analyzing important organs, such as cereal seeds, fruits, or stems sugarcane, will provide information genetic improvements increase fruit quality. This review discusses current research progress on involved carbohydrate roles accumulation also discussed highlight their contribution efficient breeding.

Language: Английский

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Sugar transporters and their molecular tradeoffs during abiotic stress responses in plants

Physiologia Plantarum, Journal Year: 2022, Volume and Issue: 174(2)

Published: Feb. 17, 2022

Sugars as photosynthates are well known energy providers and building blocks of various structural components plant cells, tissues organs. Additionally, a part sugar signaling pathways, they interact with other cellular machinery influence many important decisions in plants. Sugar is further reliant on the differential distribution sugars throughout system. The from source to sink or within organelles cells highly regulated process facilitated by transporters located plasma membranes organelle membranes, respectively. distribution, signaling, impacted during unfavorable environments such extreme temperatures, salt, nutrient scarcity, drought. Here, we have discussed mechanism transport via types their response environmental stress exposure. functional involvement plant's abiotic tolerance also discussed. Besides, highlighted challenges engineering transporter proteins undeciphered modules associated Thus, this review provides comprehensive discussion role regulation stresses enables us target candidate transporter(s) for crop improvement develop climate-resilient crops.

Language: Английский

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An update on sugar allocation and accumulation in fruits

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 193(2), P. 888 - 899

Published: May 24, 2023

Fruit sweetness is determined by the amount and composition of sugars in edible flesh. The accumulation sugar a highly orchestrated process that requires coordination numerous metabolic enzymes transporters. This enables partitioning long-distance translocation photoassimilates from source tissues to sink organs. In fruit crops, ultimately accumulate fruit. Whereas tremendous progress has been achieved understanding function individual genes associated with metabolism transport non-fruit there less known about transporters responsible for crop species. review identifies knowledge gaps can serve as foundation future studies, comprehensive updates focusing on (1) physiological roles allocation contribute crops; (2) molecular mechanisms underlying transcriptional posttranslational regulation metabolism. We also provide insights into challenges directions studies name several promising should be targeted gene editing pursuit optimized enhance fruits.

Language: Английский

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The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(3), P. 1858 - 1876

Published: March 13, 2023

Abstract Sugars are fundamental to plant developmental processes. For fruits, the accumulation and proportion of sugars play crucial roles in development quality attractiveness. In citrus (Citrus reticulata Blanco.), we found that difference sweetness between mature fruits “Gongchuan” its bud sport “Youliang” is related hexose contents. Expression a SuS (sucrose synthase) gene CitSUS5 SWEET (sugars will eventually be exported transporter) CitSWEET6, characterized by transcriptome analysis at different stages these 2 varieties, revealed higher expression levels fruit. The CitSWEET6 were investigated enzyme activity transient assays. promoted cleavage sucrose hexoses, was identified as fructose transporter. Further investigation transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) contributes metabolism transportation positively regulating CitSWEET6. role fruit sugar homologous overexpression, -VIGS, -RNAi. modulates mediating expression, molecular mechanism explained differences composition

Language: Английский

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Abscisic acid and regulation of the sugar transporter gene MdSWEET9b promote apple sugar accumulation

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 192(3), P. 2081 - 2101

Published: Feb. 23, 2023

Abstract Enhancing fruit sugar contents, especially for high-flavonoid apples with a sour taste, is one of the main goals horticultural crop breeders. This study analyzed accumulation and underlying mechanisms in F2 progenies hybridization between high-sugar apple (Malus × domestica) variety “Gala” germplasm “CSR6R6”. We revealed that MdSWEET9b (sugars will eventually be exported transporter) helps mediate fruits. Functional characterization yeast mutants lacking transport as well overexpressing CRISPR/Cas9 knockdown calli could sucrose specifically, ultimately promoting normal growth total contents. Moreover, MdWRKY9 bound to promoter regulated its activity, which responded abscisic acid (ABA) signaling. Furthermore, interacted MdbZIP23 (basic leucine zipper) MdbZIP46, key ABA signal transducers, at protein DNA levels enhance regulatory effect on expression, thereby influencing accumulation. Based contents lines differing effects treatments fruits calli, we factors responsible diversity content. The results this have clarified how influences accumulation, while also further elucidating ABA-signaling network work provides basis future explorations crosstalk hormone metabolism pathways.

Language: Английский

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Structure, evolution, and roles of SWEET proteins in growth and stress responses in plants

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130441 - 130441

Published: Feb. 28, 2024

Language: Английский

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