Organic Letters,
Journal Year:
2024,
Volume and Issue:
26(20), P. 4302 - 4307
Published: May 10, 2024
A
plant
used
in
an
Indonesian
traditional
herbal
medicine
as
a
diabetes
treatment
and
known
locally
"Jampu
Salo"
was
collected
on
Sulawesi
Island,
Indonesia.
It
identified
Syzygium
oblanceolatum
(C.
B.
Rob.)
Merr.
(Myrtaceae)
found
for
the
first
time
Sulawesi;
it
previously
reported
only
eastern
Philippines
Borneo.
phytochemical
study
of
S.
led
to
isolation
three
unprecedented
meroterpenoids,
syzygioblanes
A–C
(1–3,
respectively).
These
compounds
might
be
biosynthesized
through
[4+2]
cycloaddition
various
germacrane-based
cyclic
sesquiterpenoids
with
flavone
desmethoxymatteucinol
form
spiro
skeleton.
The
unique
complex
structures
were
elucidated
by
microcrystal
electron
diffraction
analysis
addition
general
analytical
techniques
such
high-resolution
mass
spectrometry,
nuclear
magnetic
resonance
methods,
infrared
spectroscopy.
Synchrotron
X-ray
calculations
electronic
circular
dichroism
spectra
helped
determine
absolute
configurations.
newly
isolated
exhibited
collateral
sensitivity
more
strongly
inhibit
growth
multidrug
resistant
tumor
cell
line
compared
chemosensitive
line.
Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(8), P. 3314 - 3329
Published: Feb. 21, 2022
Natural
products
are
the
result
of
Nature's
exploration
biologically
relevant
chemical
space
through
evolution
and
an
invaluable
source
bioactive
small
molecules
for
biology
medicinal
chemistry.
Novel
concepts
discovery
new
compound
classes
based
on
natural
product
structure
may
enable
wider
space.
The
pseudo-natural
concept
merges
relevance
with
efficient
by
means
fragment-based
development
to
inspire
matter
de
novo
combination
fragments
in
unprecedented
arrangements.
novel
scaffolds
retain
biological
but
not
obtainable
known
biosynthetic
pathways
which
can
lead
chemotypes
that
have
unexpected
or
bioactivities.
Herein,
we
cover
workflow
design
development,
highlight
recent
examples,
discuss
a
cheminformatic
analysis
significant
portion
active
synthetic
compounds
were
found
be
products.
We
compare
as
human-made
equivalent,
i.e.
structure.
Natural Product Reports,
Journal Year:
2024,
Volume and Issue:
41(5), P. 748 - 783
Published: Jan. 1, 2024
This
review
covers
all
fungal
terpenoid
cyclases
(TCs),
including
class
I
and
II
TCs
as
well
emerging
UbiA-type
TCs,
together
with
their
tailoring
enzymes,
focusing
on
newly
identified
enzymes
from
2015
to
August
2023.
Angewandte Chemie International Edition,
Journal Year:
2019,
Volume and Issue:
58(45), P. 15964 - 15976
Published: June 11, 2019
This
Minireview
summarises
recent
developments
in
the
biosynthesis
of
diterpenes
by
diterpene
synthases
bacteria.
It
is
structured
class
enzyme
involved
first
committed
step
towards
diterpenes,
starting
with
type
I
synthases,
followed
II
enzymes
and
more
recently
discovered
UbiA-related
synthases.
A
special
emphasis
lies
on
reaction
mechanisms
that
convert
simple
linear
precursors
through
cationic
cascades
into
structurally
complex,
usually
polycyclic
carbon
skeletons
multiple
stereogenic
centres.
further
main
focus
this
a
discussion
how
these
can
be
unravelled.
Downstream
modifications
to
bioactive
molecules
are
also
covered.
Journal of the American Chemical Society,
Journal Year:
2019,
Volume and Issue:
141(36), P. 14052 - 14056
Published: Aug. 28, 2019
Diels–Alder
reactions
are
among
the
most
powerful
synthetic
transformations
to
construct
complex
natural
products.
Despite
that
increasing
of
enzymatic
intramolecular
have
been
discovered,
intermolecular
Diels–Alderases
rarely
described.
Here,
we
report
an
hetero-Diels–Alder
reaction
in
biosynthesis
tropolonic
sesquiterpenes
and
functionally
characterize
EupfF
as
first
fungal
hetero-Diels–Alderase.
We
demonstrate
catalyzed
dehydration
a
hydroxymethyl-containing
tropolone
(5)
generate
reactive
o-quinone
methide
(6)
might
further
stereoselectively
control
subsequent
with
(1E,4E,8Z)-humulenol
(8)
produce
enantiomerically
pure
neosetophomone
B
(1).
Our
results
reveal
biosynthetic
pathway
1
expand
repertoire
activities
cyclases.
Nature Communications,
Journal Year:
2018,
Volume and Issue:
9(1)
Published: Sept. 20, 2018
Abstract
Strobilurins
from
fungi
are
the
inspiration
for
creation
of
β-methoxyacrylate
class
agricultural
fungicides.
However,
molecular
details
biosynthesis
strobilurins
have
remained
cryptic.
Here
we
report
sequence
genomes
two
that
produce
and
show
each
contains
a
biosynthetic
gene
cluster,
which
encodes
highly
reducing
polyketide
synthase
with
very
unusual
C-terminal
hydrolase
methyltransferase
domains.
Expression
stpks1
in
Aspergillus
oryzae
leads
to
production
prestrobilurin
A
when
fermentation
is
supplemented
benzoyl
coenzyme
(CoA)
analogue.
This
enables
discovery
previously
unobserved
route
CoA.
Reconstruction
cluster
A.
formation
A,
addition
str9
encoding
an
FAD-dependent
oxygenase
key
oxidative
rearrangement
responsible
toxophore.
Finally,
methyltransferases
required
complete
synthesis.
Natural Product Reports,
Journal Year:
2018,
Volume and Issue:
36(8), P. 1137 - 1155
Published: Dec. 17, 2018
Covering:
2008
to
2018
This
review
provides
a
comprehensive
overview
of
newly
discovered
natural
products
containing
tropolonoid
motif
covering
up
and
depicts
the
ecological
context
in
which
they
have
been
isolated.
has
strong
focus
on
describing
different
analytical
tools
molecular
biological
approaches
used
identify
underlying
biosynthetic
pathways.
Natural Product Reports,
Journal Year:
2021,
Volume and Issue:
38(8), P. 1469 - 1505
Published: Jan. 1, 2021
Cytochrome
P450s,
laccases,
and
intermolecular
[4
+
2]
cyclases,
along
with
other
enzymes
were
utilized
to
catalyze
varied
dimerization
of
matured
natural
products
so
as
create
the
structural
diversity
complexity
in
microorganisms.
Natural Product Reports,
Journal Year:
2022,
Volume and Issue:
40(1), P. 89 - 127
Published: Sept. 20, 2022
Covering:
2010
to
2022Heterologous
expression
of
natural
product
biosynthetic
gene
clusters
(BGCs)
has
become
a
widely
used
tool
for
genome
mining
cryptic
pathways,
bottom-up
investigation
enzymes,
and
engineered
biosynthesis
new
variants.
In
the
field
fungal
products,
heterologous
complete
pathway
was
first
demonstrated
in
tenellin
