Harnessing extracellular vesicle heterogeneity for diagnostic and therapeutic applications DOI
Randy P. Carney, Rachel R. Mizenko, Batuhan Turhan Bozkurt

et al.

Nature Nanotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Language: Английский

RNA delivery by extracellular vesicles in mammalian cells and its applications DOI Open Access
Killian P. O’Brien, Koen Breyne, Stefano Ughetto

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(10), P. 585 - 606

Published: May 26, 2020

Language: Английский

Citations

1427
Extracellular vesicles as a next-generation drug delivery platform DOI Open Access
Inge K. Herrmann, Matthew J. A. Wood, Gregor Fuhrmann

et al.

Nature Nanotechnology, Journal Year: 2021, Volume and Issue: 16(7), P. 748 - 759

Published: July 1, 2021

Language: Английский

Citations

1353
The exosome journey: from biogenesis to uptake and intracellular signalling DOI Creative Commons
Sonam Gurung, Dany Perocheau,

Loukia Touramanidou

et al.

Cell Communication and Signaling, Journal Year: 2021, Volume and Issue: 19(1)

Published: April 23, 2021

Abstract The use of exosomes in clinical settings is progressively becoming a reality, as trials testing for diagnostic and therapeutic applications are generating remarkable interest from the scientific community investors. Exosomes small extracellular vesicles secreted by all cell types playing intercellular communication roles health disease transferring cellular cargoes such functional proteins, metabolites nucleic acids to recipient cells. An in-depth understanding exosome biology therefore essential ensure development based investigational products. Here we summarise most up-to-date knowkedge about complex biological journey biogenesis secretion, transport uptake their intracellular signalling. We delineate major pathways molecular players that influence each step physiology, highlighting routes interest, which will be benefit manipulation engineering. highlight main controversies field research: adequate definition, characterisation at plasma membrane. also common identified pitfalls affecting research development. Unravelling physiology key ultimate progression towards applications.

Language: Английский

Citations

1202
Engineering exosomes for targeted drug delivery DOI Creative Commons
Yujie Liang, Li Duan, Jianping Lu

et al.

Theranostics, Journal Year: 2021, Volume and Issue: 11(7), P. 3183 - 3195

Published: Jan. 1, 2021

Exosomes are cell-derived nanovesicles that involved in the intercellular transportation of materials. Therapeutics, such as small molecules or nucleic acid drugs, can be incorporated into exosomes and then delivered to specific types cells tissues realize targeted drug delivery. Targeted delivery increases local concentration therapeutics minimizes side effects. Here, we present a detailed review engineering through genetic chemical methods for Although still its infancy, exosome-mediated boasts low toxicity, immunogenicity, high engineerability, holds promise cell-free therapies wide range diseases.

Language: Английский

Citations

993
Challenges and directions in studying cell–cell communication by extracellular vesicles DOI
Guillaume van Niel,

David R. F. Carter,

Aled Clayton

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(5), P. 369 - 382

Published: March 8, 2022

Language: Английский

Citations

807
Context-specific regulation of extracellular vesicle biogenesis and cargo selection DOI
Andrew C. Dixson,

T. Renee Dawson,

Dolores Di Vizio

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 454 - 476

Published: Feb. 10, 2023

Language: Английский

Citations

390
The Machinery of Exosomes: Biogenesis, Release, and Uptake DOI Open Access
Sofia V. Krylova,

Daorong Feng

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1337 - 1337

Published: Jan. 10, 2023

Exosomes are a subtype of membrane-contained vesicles 40–200 nm in diameter that secreted by cells into their surroundings. By transporting proteins, lipids, mRNA, miRNA, lncRNA, and DNA, exosomes able to perform such vital functions as maintaining cellular homeostasis, removing debris, facilitating intercellular interorgan communication. travel all body fluids deliver molecular messages autocrine, paracrine well endocrine manners. In recent years, there has been an increased interest studying diagnostic markers therapeutic targets, since many disease conditions this machinery becomes dysregulated or hijacked pathological processes. Additionally, delivery exosomal miRNA already shown improve systemic metabolism inhibit progression cancer development mice. However, the subcellular exosomes, including biogenesis, release uptake, remains largely unknown. This review will bring details these processes up date with goal expanding knowledge basis for designing impactful exosome experiments future.

Language: Английский

Citations

280
The power of imaging to understand extracellular vesicle biology in vivo DOI
Frederik J. Verweij, Leonora Balaj, Chantal M. Boulanger

et al.

Nature Methods, Journal Year: 2021, Volume and Issue: 18(9), P. 1013 - 1026

Published: Aug. 26, 2021

Language: Английский

Citations

266
Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles DOI
Guillermo Bordanaba-Florit,

Félix Royo,

Sergei G. Kruglik

et al.

Nature Protocols, Journal Year: 2021, Volume and Issue: 16(7), P. 3163 - 3185

Published: June 16, 2021

Language: Английский

Citations

216
The ins-and-outs of exosome biogenesis, secretion, and internalization DOI
Subhash B. Arya,

Samuel P. Collie,

Carole A. Parent

et al.

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 34(2), P. 90 - 108

Published: July 27, 2023

Language: Английский

Citations

158