AuNPs/CNC Nanocomposite with A “Dual Dispersion” Effect for LDI‐TOF MS Analysis of Intact Proteins in NSCLC Serum Exosomes DOI Creative Commons
Liang Shan, Yongxia Qiao,

Lifang Ma

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(12)

Published: Jan. 15, 2024

Abstract Detecting exosomal markers using laser desorption/ionization time‐of‐flight mass spectrometry (LDI‐TOF MS) is a novel approach for examining liquid biopsies of non‐small cell lung cancer (NSCLC) samples. However, LDI‐TOF MS limited by low sensitivity and poor reproducibility when analyzing intact proteins directly. In this report, gold nanoparticles/cellulose nanocrystals (AuNPs/CNC) introduced as the matrix direct analysis in NSCLC serum exosomes. AuNPs/CNC with “dual dispersion” effects dispersed stabilized AuNPs improved ion inhibition caused protein aggregation. These features increased signal‐to‐noise ratio [M+H] + peaks two orders magnitude lowered detection limit to 0.01 mg mL –1 . The coefficient variation or without measured 10.2% 32.5%, respectively. excellent yielded linear relationship ( y = 15.41 x – 7.983, R 2 0.989) over concentration range 20 Finally, AuNPs/CNC‐assisted provides clinically relevant fingerprint information serum, characteristic S100 calcium‐binding A10, Urokinase plasminogen activator surface receptor, Plasma protease C1 inhibitor, Tyrosine‐protein kinase Fgr Mannose‐binding lectin associated serine represented predictive biomarkers risk.

Language: Английский

Cancer Nanovaccines: Nanomaterials and Clinical Perspectives DOI
Nimeet Desai, Vivek P. Chavda, Thakur Raghu Raj Singh

et al.

Small, Journal Year: 2024, Volume and Issue: 20(35)

Published: May 1, 2024

Abstract Cancer nanovaccines represent a promising frontier in cancer immunotherapy, utilizing nanotechnology to augment traditional vaccine efficacy. This review comprehensively examines the current state‐of‐the‐art nanovaccine development, elucidating innovative strategies and technologies employed their design. It explores both preclinical clinical advancements, emphasizing key studies demonstrating potential elicit robust anti‐tumor immune responses. The study encompasses various facets, including integrating biomaterial‐based nanocarriers for antigen delivery, adjuvant selection, impact of nanoscale properties on performance. Detailed insights into complex interplay between tumor microenvironment responses are provided, highlighting challenges opportunities optimizing therapeutic outcomes. Additionally, presents thorough analysis ongoing trials, presenting snapshot landscape. By curating latest scientific findings developments, this aims serve as comprehensive resource researchers clinicians engaged advancing immunotherapy. Integrating design holds immense promise revolutionizing treatment paradigms, provides timely update evolving landscape nanovaccines.

Language: Английский

Citations

24
The Discovery of Extracellular Vesicles and Their Emergence as a Next-Generation Therapy DOI
Alin Rai, Bethany Claridge, Jonathan Lozano

et al.

Circulation Research, Journal Year: 2024, Volume and Issue: 135(1), P. 198 - 221

Published: June 20, 2024

From their humble discovery as cellular debris to cementing natural capacity transfer functional molecules between cells, the long-winded journey of extracellular vesicles (EVs) now stands at precipice a next-generation cell-free therapeutic tool revolutionize modern-day medicine. This perspective provides snapshot EVs emergence vibrant field biology and renaissance they usher in biomedical sciences agents for cardiovascular pathologies. Rapid development bioengineered is providing innovative opportunities overcome biological challenges such potency, cargo loading enhanced secretion, targeting circulation half-life, localized sustained delivery strategies, approaches enhance systemic circulation, uptake lysosomal escape, logistical hurdles encompassing scalability, cost, time. A multidisciplinary collaboration beyond extends chemistry, physics, biomaterials, nanotechnology, allowing rapid designer that are entering late-stage human clinical trials.

Language: Английский

Citations

19
Emerging role of exosomes in cancer therapy: progress and challenges DOI Creative Commons
Jiale Li,

Jiachong Wang,

Zigui Chen

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 13, 2025

This review highlights recent progress in exosome-based drug delivery for cancer therapy, covering exosome biogenesis, cargo selection mechanisms, and their application across multiple types. As small extracellular vesicles, exosomes exhibit high biocompatibility low immunogenicity, making them ideal vehicles capable of efficiently targeting cells, minimizing off-target damage side effects. aims to explore the potential with a focus on applications chemotherapy, gene immunomodulation. Additionally, challenges related production standardization are analyzed, highlighting importance addressing these issues clinical application. In conclusion, systems offer promising future therapies. Further research should aim enhance efficiency facilitate translation, paving way innovative treatment strategies.

Language: Английский

Citations

19
Exosome-mediated tumor metastasis: Biology, molecular targets and immuno-therapeutic options DOI

Reza Moshrefiravasjani,

Amin Kamrani,

Nazanin Nazari

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 254, P. 155083 - 155083

Published: Jan. 3, 2024

Language: Английский

Citations

18
Cancer-associated fibroblast-derived extracellular vesicles: regulators and therapeutic targets in the tumor microenvironment DOI Open Access
Jindong Xie, Xinmei Lin, Xinpei Deng

et al.

Cancer Drug Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Cancer-associated fibroblasts (CAFs) constitute a critical component of the tumor microenvironment (TME). CAFs can be reprogrammed by cancer cells, leading to production extracellular vesicles (EVs). These EVs serve as carriers for bioactive substances, including proteins, nucleic acids, and metabolic products, thereby facilitating progression. CAF-derived exert substantial influence on cell proliferation, invasion, metastasis, immunological environment, processes lymphangiogenesis angiogenesis. Despite their potential non-invasive biomarkers therapeutic delivery vehicles, clinical application is currently limited challenges in purification precise targeting. This review delineates diverse roles growth, immune evasion within TME.

Language: Английский

Citations

2
Exploring extracellular RNA as drivers of chemotherapy resistance in cancer DOI

Yumna Khan,

Md Sadique Hussain, Prasanna Srinivasan Ramalingam

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 21, 2025

Language: Английский

Citations

2
Adipose stem cells in tissue regeneration and repair: From bench to bedside DOI Creative Commons
Lei Dong, Xiaoyu Li,

Wenyuan Leng

et al.

Regenerative Therapy, Journal Year: 2023, Volume and Issue: 24, P. 547 - 560

Published: Oct. 11, 2023

ADSCs are a large number of mesenchymal stem cells in Adipose tissue, which can be applied to tissue engineering. have the potential multi-directional differentiation, and differentiate into bone cardiac urothelial cells, skin etc. Compared with other multitude promising advantages, such as abundant number, accessibility cell culture, stable function, less immune rejection. There two main methods use for repair regeneration. One is implant "ADSCs-scaffold composite" injured site promote The cell-free therapy: using ADSC-exos or ADSC-CM alone release miRNAs, cytokines bioactive substances regeneration regulated by variety cytokines, signaling molecules, external environment. differentiation different tissues also induced growth factors, ions, hormones, scaffold materials, physical stimulation, factors. specific mechanisms complex, most pathways need further explored. This article reviews summarizes mechanism clinical application injury so far, puts forward problems that solved this field, hoping provide directions research field.

Language: Английский

Citations

25
Macrophage-Derived Exosomes as Advanced Therapeutics for Inflammation: Current Progress and Future Perspectives DOI Creative Commons
Y. Song, Jing Hu,

Chunlian Ma

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 1597 - 1627

Published: Feb. 1, 2024

Abstract: The development of numerous diseases is significantly influenced by inflammation. Macrophage-derived exosomes (M-Exos) play a role in controlling inflammatory reactions various conditions, including chronic pain, hypertension, and diabetes. However, the specific targets roles M-Exos regulating inflammation remain largely unknown. This review summarizes current knowledge on biogenesis provides updated information M-Exos' biological function modulation. Furthermore, this highlights functionalization engineering strategies M-Exos, while providing an overview cutting-edge approaches to advancements their application as therapeutics for Finally, multiple mechanisms are presented along with perspectives challenges, potential contribution that may have through modulation discussed. Keywords: inflammation, macrophage-derived exosomes, functions, modulation,

Language: Английский

Citations

14
Exosome-based nanoparticles and cancer immunotherapy DOI Open Access
Jiarong Ye, Danni Li,

Yiting Jie

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117296 - 117296

Published: Aug. 20, 2024

Over the past decades, cancer immunotherapy has encountered challenges such as immunogenicity, inefficiency, and cytotoxicity. Consequently, exosome-based gained rapid traction a promising alternative. Exosomes, type of extracellular vesicles (EVs) ranging from 50 to 150 nm, are self-originating exhibit fewer side effects compared traditional therapies. Exosome-based encompasses three significant areas: vaccination, co-inhibitory checkpoints, adoptive T-cell therapy. Each these fields leverages inherent advantages exosomes, demonstrating substantial potential for individualized tumor therapy precision medicine. This review aims elucidate reasons behind promise nanoparticles therapies by examining their characteristics summarizing latest research advancements in immunotherapy.

Language: Английский

Citations

10
The hidden messengers: cancer associated fibroblasts—derived exosomal miRNAs as key regulators of cancer malignancy DOI Creative Commons
Zixuan Gou, Jiannan Li, Jianming Liu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: April 17, 2024

Cancer-associated fibroblasts (CAFs), a class of stromal cells in the tumor microenvironment (TME), play key role controlling cancer cell invasion and metastasis, immune evasion, angiogenesis, resistance to chemotherapy. CAFs mediate their activities by secreting soluble chemicals, releasing exosomes, altering extracellular matrix (ECM). Exosomes contain various biomolecules, such as nucleic acids, lipids, proteins. microRNA (miRNA), 22–26 nucleotide non-coding RNA, can regulate cellular transcription processes. Studies have shown that miRNA-loaded exosomes secreted engage regulatory communication networks with other TME constituents. This study focused on roles CAF-derived exosomal miRNAs generating malignant characteristics, including modulation, growth, migration invasion, epithelial-mesenchymal transition (EMT), treatment resistance. thoroughly examines miRNA’s dual promoting suppressing cancer. Thus, changes be used biomarkers for diagnosis prognosis patients, specificity develop newer therapies. review also discusses pressing problems require immediate attention, aiming inspire researchers explore more novel avenues this field.

Language: Английский

Citations

9