Recent advances in targeted antibacterial therapy basing on nanomaterials

Exploration, Journal Year: 2023, Volume and Issue: 3(1)

Published: Feb. 1, 2023

Bacterial infection has become one of the leading causes death worldwide, particularly in low-income countries. Despite fact that antibiotics have provided successful management bacterial infections, long-term overconsumption and abuse contributed to emergence multidrug resistant bacteria. To address this challenge, nanomaterials with intrinsic antibacterial properties or serve as drug carriers been substantially developed an alternative fight against infection. Systematically deeply understanding mechanisms is extremely important for designing new therapeutics. Recently, nanomaterials-mediated targeted bacteria depletion either a passive active manner most promising approaches treatment by increasing local concentration around cells enhance inhibitory activity reduce side effects. Passive targeting approach widely explored searching nanomaterial-based alternatives antibiotics, while strategy relies on biomimetic biomolecular surface feature can selectively recognize In review article, we summarize recent developments field therapy based nanomaterials, which will promote more innovative thinking focusing multidrug-resistant

Language: Английский

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Bacteria loaded with glucose polymer and photosensitive ICG silicon-nanoparticles for glioblastoma photothermal immunotherapy

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 1, 2022

Abstract Bacteria can bypass the blood-brain barrier (BBB), suggesting possibility of employment bacteria for combating central nervous system diseases. Herein, we develop a bacteria-based drug delivery glioblastoma (GBM) photothermal immunotherapy. The system, which name as ‘Trojan bacteria’, consists loaded with glucose polymer and photosensitive ICG silicon-nanoparticles. In an orthotopic GBM mouse model, demonstrate that intravenously injected BBB, targeting penetrating tissues. Upon 808 nm-laser irradiation, effects produced by allow destruction bacterial cells adjacent tumour cells. Furthermore, debris well tumour-associated antigens promote antitumor immune responses prolong survival GBM-bearing mice. Moreover, residual are effectively eliminated from body, supporting potential therapeutic use this system.

Language: Английский

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Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 23, 2023

Abstract Micro-nano biorobots based on bacteria have demonstrated great potential for tumor diagnosis and treatment. The bacterial gene expression drug release should be spatiotemporally controlled to avoid in healthy tissues undesired toxicity. Herein, we describe an alternating magnetic field-manipulated tumor-homing developed by genetically modifying engineered Escherichia coli with Fe 3 O 4 @lipid nanocomposites. After accumulating orthotopic colon tumors female mice, the paramagnetic nanoparticles enable receive convert signals into heat, thereby initiating of lysis proteins under control a heat-sensitive promoter. then lyse, releasing its anti-CD47 nanobody cargo, that is pre-expressed within bacteria. robust immunogenicity lysate cooperates activate both innate adaptive immune responses, generating antitumor effects against not only but also distal mice. magnetically constant field-controlled motion enhanced targeting increased therapeutic efficacy. Thus, behavior can manipulated vivo field, achieving tumor-specific CD47 blockage precision immunotherapy.

Language: Английский

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Decorated bacteria and the application in drug delivery

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 188, P. 114443 - 114443

Published: July 8, 2022

Language: Английский

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Coupling Probiotics with 2D CoCuMo‐LDH Nanosheets as a Tumor‐Microenvironment‐Responsive Platform for Precise NIR‐II Photodynamic Therapy

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(23)

Published: March 13, 2023

Photodynamic therapy (PDT) has become a promising cancer treatment approach with superior advantages. However, it remains grand challenge to develop tumor microenvironment (TME)-responsive photosensitizers (PSs) for tumor-targeting precise PDT. Herein, the coupling Lactobacillus acidophilus (LA) probiotics 2D CoCuMo layered-double-hydroxide (LDH) nanosheets (LA&LDH) is reported as TME-responsive platform NIR-II The CoCuMo-LDH loaded on LA can be transformed from crystalline into amorphous through etching by LA-metabolite-enabled low pH and overexpressed glutathione. TME-induced in situ amorphization of boost its photodynamic activity singlet oxygen (1 O2 ) generation under 1270 nm laser irradiation relative 1 quantum yield 1.06, which highest among previously NIR-excited PSs. In vitro vivo assays prove that LA&LDH effectively achieve complete cell apoptosis eradication irradiation. This study proves used highly efficient

Language: Английский

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Decorating Bacteria with Triple Immune Nanoactivators Generates Tumor‐Resident Living Immunotherapeutics

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(27)

Published: April 11, 2022

An approach of decorating bacteria with triple immune nanoactivators is reported to develop tumor-resident living immunotherapeutics. Under cytocompatible conditions, tumor-specific antigens and checkpoint blocking antibodies are simultaneously conjugated onto bacterial surface then polydopamine nanoparticles formed via in situ dopamine polymerization. In addition serving as a linker, its photothermal effect can repolarize tumor-associated macrophages pro-inflammatory phenotype. The linked promote the maturation dendritic cells generate responses, while anchored block checkpoints activate cytotoxic T lymphocytes. Decorated show spatiotemporal tumor retention proliferation-dependent drug release, achieving potent antitumor effects two antigen-overexpressing models. This work provides versatile platform prepare multimodal long-acting therapeutics for cancer immunotherapy.

Language: Английский

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An Engineered Bacteria-Hybrid Microrobot with the Magnetothermal Bioswitch for Remotely Collective Perception and Imaging-Guided Cancer Treatment

ACS Nano, Journal Year: 2022, Volume and Issue: 16(4), P. 6118 - 6133

Published: March 28, 2022

Microrobots driven by multiple propelling forces hold great potential for noninvasively targeted delivery in the physiologic environment. However, remotely collective perception and precise a low Reynold's number bioenvironment remain major challenges of microrobots to achieve desired therapeutic effects vivo. Here, we reported biohybrid microrobot that integrated with magnetic, thermal, hypoxia sensitivities an internal fluorescent protein as dual reporter thermal positioning signals cancer treatment. There were three key elements microrobotic system, including magnetic nanoparticle (MNP)-loaded probiotic Escherichia coli Nissle1917 (EcN@MNP) spatially perception, thermal-logic circuit engineered into bacteria control biosynthesis mCherry temperature reporter, NDH-2 enzyme encoded EcN enhanced anticancer therapy. According fluorescent-protein-based imaging feedback, showed good sensitivity active targeting ability tumor area manner under field. The cell apoptosis was efficiently triggered vitro vivo hybrid coupled magnetothermal ablation NDH-2-induced reactive oxygen species (ROS) damage. Our study demonstrates is ideal platform integrate physical, biological, chemical properties

Language: Английский

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Enhancing Gasdermin-induced tumor pyroptosis through preventing ESCRT-dependent cell membrane repair augments antitumor immune response

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 24, 2022

Pore-forming Gasdermin protein-induced pyroptosis in tumor cells promotes anti-tumor immune response through the release of pro-inflammatory cytokines and immunogenic substances after cell rupture. However, endosomal sorting complexes required for transport (ESCRT) III-mediated membrane repair significantly diminishes by repairing subsequently removing gasdermin pores. Here, we show that blocking calcium influx-triggered ESCRT III-dependent a biodegradable nanoparticle-mediated sustained chelator (EI-NP) strongly enhances intracellularly delivered GSDMD-induced via bacteria-based delivery system (VNP-GD). An injectable hydrogel lyophilized hydrogel-based patch are developed peritumoral administration treating primary metastatic tumors, implantation inoperable tumors respectively. The hydrogels, functioning as local therapeutic reservoirs, can sustainedly VNP-GD to effectively trigger EI-NP prevent III-induced plasma boost effects, working synergistically augment response.

Language: Английский

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Recent progress of aptamer‒drug conjugates in cancer therapy

Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 13(4), P. 1358 - 1370

Published: Jan. 26, 2023

Aptamers are single-stranded DNA or RNA sequences that can specifically bind with the target protein molecule

Language: Английский

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Integrating Bacteria with a Ternary Combination of Photosensitizers for Monochromatic Irradiation-Mediated Photoacoustic Imaging-Guided Synergistic Photothermal Therapy

ACS Nano, Journal Year: 2023, Volume and Issue: 17(5), P. 5059 - 5071

Published: Feb. 27, 2023

Photosensitizer-based therapy often suffers from unitary and easily attenuated photosensitive effects, limited tumor penetration retention, requirement of multiple irradiation for combination therapy, which largely restrict its application. Here, bacteria are integrated with a monochromatic irradiation-mediated ternary photosensitizers photoacoustic imaging-guided synergistic photothermal therapy. Bacteria that bioengineered to express natural melanin decorated dual synthetic by nanodeposition indocyanine green polydopamine under cytocompatible condition. The combined photosensitizers, share an adequate excitation at 808 nm, endow stable triple effect irradiation. Due their living characteristics, these preferentially colonize hypoxic tissue homogeneous distribution durable retention generate uniform imaging signals sufficient heating upon laser Supported significantly inhibited growth extended survival animals in different tumor-bearing murine models, our work proposes the development bacteria-based innovative

Language: Английский

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