Image‐Guided Photothermal and Immune Therapy of Tumors via Melanin‐Producing Genetically Engineered Bacteria DOI Creative Commons
Weijian Song, Yaling He, Ya‐Nan Feng

et al.

Small, Journal Year: 2024, Volume and Issue: 20(26)

Published: Feb. 17, 2024

Abstract Photothermal therapy (PTT) is a new treatment modality for tumors. However, the efficient delivery of photothermal agents into tumors remains difficult, especially in hypoxic tumor regions. In this study, an approach to deliver melanin, natural agent, using genetically engineered bacteria image‐guided and immune developed. An Escherichia coli MG1655 transformed with recombinant plasmid harboring tyrosinase gene produce melanin nanoparticles. Melanin‐producing (MG1655‐M) are systemically administered 4T1 tumor‐bearing mice. The tumor‐targeting properties MG1655‐M environment integrate hypoxia targeting, photoacoustic imaging, therapeutic “all‐in‐one” manner. This eliminates need post‐modification achieve hypoxia‐targeted cancer therapy. Tumor growth significantly suppressed by irradiating 808 nm laser. Furthermore, strong antitumor immunity triggered PTT, thereby producing long‐term memory effects that effectively inhibit metastasis recurrence. work proposes guided melanin‐producing bacteria, which can offer broad potential applications treatment.

Language: Английский

Photothermal Nanomaterials: A Powerful Light-to-Heat Converter DOI Creative Commons
Ximin Cui, Qifeng Ruan, Xiaolu Zhuo

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(11), P. 6891 - 6952

Published: May 3, 2023

All forms of energy follow the law conservation energy, by which they can be neither created nor destroyed. Light-to-heat conversion as a traditional yet constantly evolving means converting light into thermal has been enduring appeal to researchers and public. With continuous development advanced nanotechnologies, variety photothermal nanomaterials have endowed with excellent harvesting capabilities for exploring fascinating prospective applications. Herein we review latest progresses on nanomaterials, focus their underlying mechanisms powerful light-to-heat converters. We present an extensive catalogue nanostructured materials, including metallic/semiconductor structures, carbon organic polymers, two-dimensional materials. The proper material selection rational structural design improving performance are then discussed. also provide representative overview techniques probing photothermally generated heat at nanoscale. finally recent significant developments applications give brief outlook current challenges future directions nanomaterials.

Language: Английский

Citations

835
The blood–brain barrier: Structure, regulation and drug delivery DOI Creative Commons
Di Wu, Qi Chen, Xiaojie Chen

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: May 25, 2023

Abstract Blood–brain barrier (BBB) is a natural protective membrane that prevents central nervous system (CNS) from toxins and pathogens in blood. However, the presence of BBB complicates pharmacotherapy for CNS disorders as most chemical drugs biopharmaceuticals have been impeded to enter brain. Insufficient drug delivery into brain leads low therapeutic efficacy well aggravated side effects due accumulation other organs tissues. Recent breakthrough materials science nanotechnology provides library advanced with customized structure property serving powerful toolkit targeted delivery. In-depth research field anatomical pathological study on further facilitates development brain-targeted strategies enhanced crossing. In this review, physiological different cells contributing are summarized. Various emerging permeability regulation crossing including passive transcytosis, intranasal administration, ligands conjugation, coating, stimuli-triggered disruption, overcome obstacle highlighted. Versatile systems ranging organic, inorganic, biologics-derived their synthesis procedures unique physio-chemical properties summarized analyzed. This review aims provide an up-to-date comprehensive guideline researchers diverse fields, offering perspectives system.

Language: Английский

Citations

629
Nanomaterials for photothermal cancer therapy DOI Creative Commons

Shufan Duan,

Yanling Hu, Ying Zhao

et al.

RSC Advances, Journal Year: 2023, Volume and Issue: 13(21), P. 14443 - 14460

Published: Jan. 1, 2023

This review summarizes the common inorganic and organic photothermal nanoagents their applications in tumor therapy. Additionally, challenges future prospects of nanomaterial-based therapy cancer treatment are discussed.

Language: Английский

Citations

78
Cancer chemotherapy resistance: Mechanisms and recent breakthrough in targeted drug delivery DOI
Fatemeh Davodabadi, Seyedeh Fatemeh Sajjadi, Mohammad Sarhadi

et al.

European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 958, P. 176013 - 176013

Published: Aug. 24, 2023

Language: Английский

Citations

60
Engineered Bacteria Labeled with Iridium(III) Photosensitizers for Enhanced Photodynamic Immunotherapy of Solid Tumors DOI

Wenkai Lin,

Yu Liu, Jinhui Wang

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(43)

Published: Sept. 5, 2023

Despite metal-based photosensitizers showing great potential in photodynamic therapy for tumor treatment, the application of is intrinsically limited by their poor cancer-targeting properties. Herein, we reported a photosensitizer-bacteria hybrid, Ir-HEcN, via covalent labeling an iridium(III) photosensitizer to surface genetically engineered bacteria. Due its intrinsic self-propelled motility and hypoxia tropism, Ir-HEcN selectively targets penetrates deeply into tissues. Importantly, capable inducing pyroptosis immunogenic cell death cells under irradiation, thereby remarkably evoking anti-tumor innate adaptive immune responses vivo leading regression solid tumors combinational immunotherapy. To best our knowledge, first metal complex decorated bacteria enhanced

Language: Английский

Citations

52
Photothermal Heating‐Assisted Superior Antibacterial and Antibiofilm Activity of High‐Entropy‐Alloy Nanoparticles DOI
Yixing Li,

Linlin Yang,

Yijun Liao

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(35)

Published: May 17, 2023

Abstract Antibacterial elements and non‐contact heating abilities have been proven effective for antibacterial antibiofilm activities, but it remains a challenge to integrate both within one material. Herein, assisted by the high‐entropy effect, FeNiTiCrMnCu x alloy nanoparticles (HEA‐NPs) with excellent photothermal properties boosting performances are synthesized. Benefitting from synergetic effect of copper ions released thermal damage HEA‐NPs, more reactive oxygen species (ROS) generated, leading rupture cell membranes eradication biofilms. As result, efficiency (400 µg mL −1 ) mostly optimized 1.0 HEA‐NPs in marine nutrient medium, which is worst‐case scenario antimicrobial material, can be improved 81% 97.4% under 30 min solar irradiation (1 sun). The present study demonstrates new strategy effectively treating microorganisms that cause biofouling microbial corrosion using characteristics as an auxiliary.

Language: Английский

Citations

49
Upconversion dual-photosensitizer–expressing bacteria for near-infrared monochromatically excitable synergistic phototherapy DOI Creative Commons
Mian Chen, Qiuju Han, Mengmeng Zhang

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(10)

Published: March 6, 2024

Synergistic phototherapy stands for superior treatment prospects than a single phototherapeutic modality. However, the combined photosensitizers often suffer from incompatible excitation mode, limited irradiation penetration depth, and lack of specificity. We describe development upconversion dual-photosensitizer-expressing bacteria (UDPB) near-infrared monochromatically excitable combination phototherapy. UDPB are prepared by integrating genetic engineering surface modification, in which encoded to simultaneously express photothermal melanin phototoxic KillerRed protein primary amino groups derived free thiols biorthogonal conjugation nanoparticles. exhibit monochromatic irradiation-mediated dual-activation characteristic as conversion can be initiated directly, while photodynamic effect stimulated indirectly upconverted visible light emission. also show living features colonize hypoxic lesion sites inhibit pathogens via bacterial community competition. In two murine models solid tumor skin wound infection, separately induce robust antitumor response rapid healing effect.

Language: Английский

Citations

27
Receptor Ligand-Free Mesoporous Silica Nanoparticles: A Streamlined Strategy for Targeted Drug Delivery across the Blood–Brain Barrier DOI Creative Commons
Zih-An Chen, C.‐H. Wu, Si‐Han Wu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(20), P. 12716 - 12736

Published: May 8, 2024

Mesoporous silica nanoparticles (MSNs) represent a promising avenue for targeted brain tumor therapy. However, the blood–brain barrier (BBB) often presents formidable obstacle to efficient drug delivery. This study introduces ligand-free PEGylated MSN variant (RMSN25-PEG-TA) with 25 nm size and slight positive charge, which exhibits superior BBB penetration. Utilizing two-photon imaging, RMSN25-PEG-TA particles remained in circulation over 24 h, indicating significant traversal beyond cerebrovascular realm. Importantly, DOX@RMSN25-PEG-TA, our loaded doxorubicin (DOX), harnessed enhanced permeability retention (EPR) effect achieve 6-fold increase accumulation compared free DOX. In vivo evaluations confirmed potent inhibition of orthotopic glioma growth by extending survival rates spontaneous models 28% offering an improved biosafety profile. Advanced LC-MS/MS investigations unveiled distinctive protein corona surrounding RMSN25-PEG-TA, suggesting proteins such as apolipoprotein E albumin could play pivotal roles enabling its Our results underscore potential MSNs treating tumors, supports development future drug–nanoparticle design paradigms.

Language: Английский

Citations

25
Transvascular transport of nanocarriers for tumor delivery DOI Creative Commons
Xin Li, Yong Hu, Xingcai Zhang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 17, 2024

Nanocarriers (NCs) play a crucial role in delivering theranostic agents to tumors, making them pivotal focus of research. However, the persistently low delivery efficiency engineered NCs has been significant challenge advancement nanomedicine, stirring considerable debate. Transvascular transport is critical pathway for NC from vessels yet comprehensive understanding interactions between and vascular systems remains elusive. In recent years, efforts have invested elucidating transvascular mechanisms NCs, leading promising advancements tumor theranostics. this context, we highlight various mechanisms, including enhanced permeability retention effect, cooperative immune-driven active transcytosis, cell/bacteria-mediated delivery. Furthermore, explore corresponding strategies aimed at enhancing efficient These approaches offer intriguing solutions spanning physicochemical, biological, pharmacological domains improve therapeutic outcomes. Additionally, propose forward-looking framework that relies on advanced tumor/vessel models, high-throughput libraries, nano-bio interaction datasets, artificial intelligence, which aims guide design next-generation carriers implementation optimized

Language: Английский

Citations

19
Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy DOI Creative Commons
Ke‐Fei Xu,

Shun‐Yu Wu,

Zihao Wang

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 17, 2024

Abstract Bacteria-mediated cancer therapeutic strategies have attracted increasing interest due to their intrinsic tumor tropism. However, bacteria-based drugs face several challenges including the large size of bacteria and dense extracellular matrix, limiting intratumoral delivery efficiency. In this study, we find that hyperbaric oxygen (HBO), a noninvasive method, can effectively deplete matrix thus enhance bacterial accumulation within tumors. Inspired by finding, modify Escherichia coli Nissle 1917 (EcN) with cypate molecules yield EcN-cypate for photothermal therapy, which subsequently induce immunogenic cell death (ICD). Importantly, HBO treatment significantly increases facilitates infiltration immune cells realize desirable eradication through therapy ICD-induced immunotherapy. Our work provides facile strategy efficiency natural/engineered bacteria, may promote clinical translation bacteria-mediated synergistic therapy.

Language: Английский

Citations

18