Biological nitrogen fixation in cereal crops: Progress, strategies, and perspectives

Plant Communications, Journal Year: 2022, Volume and Issue: 4(2), P. 100499 - 100499

Published: Nov. 28, 2022

Nitrogen is abundant in the atmosphere but generally most limiting nutrient for plants. The inability of many crop plants, such as cereals, to directly utilize freely available atmospheric nitrogen gas means that their growth and production often rely heavily on application chemical fertilizers, which leads greenhouse emissions eutrophication water. By contrast, legumes gain access through symbiotic association with rhizobia. These bacteria convert into biologically ammonia nodules a process termed biological fixation, plays decisive role ecosystem functioning. Engineering cereal crops can fix like or associate nitrogen-fixing microbiomes could help avoid problems caused by overuse synthetic fertilizer. With development biology, various efforts have been undertaken aim creating so-called "N-self-fertilizing" capable performing autonomous fixation need fertilizers. In this review, we briefly summarize history current status engineering N-self-fertilizing crops. We also propose several potential biotechnological approaches incorporating capacity non-legume

Language: Английский

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Harnessing biological nitrogen fixation in plant leaves

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 28(12), P. 1391 - 1405

Published: June 1, 2023

Language: Английский

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Superiority of native soil core microbiomes in supporting plant growth

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 4, 2024

Native core microbiomes represent a unique opportunity to support food provision and plant-based industries. Yet, these are often neglected when developing synthetic communities (SynComs) plant health growth. Here, we study the contribution of native core, non-core non-native microorganisms production. We construct four alternative SynComs based on excellent growth promoting ability individual stain paired non-antagonistic action. One microbiome SynCom (SC2) shows high niche breadth low average variation degree in-vitro interaction. The promoting-growth effect SC2 can be transferred non-sterile environment, attributing colonization improvement rhizosphere function including nitrogen fixation, IAA production, dissolved phosphorus. Further, microbial fertilizer composite carrier (rapeseed cake + rice husk carbon) increase net biomass by 129%. Our results highlight fundamental importance boost authors show that have greater potential than both microorganisms.

Language: Английский

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Composing a microbial symphony: synthetic communities for promoting plant growth

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Microbiomes in agroecosystem: Diversity, function and assembly mechanisms

Environmental Microbiology Reports, Journal Year: 2022, Volume and Issue: 14(6), P. 833 - 849

Published: Oct. 2, 2022

Abstract Soils are a main repository of biodiversity harbouring immense diversity microbial species that plays central role in fundamental ecological processes and acts as the seed bank for emergence plant microbiome cropland ecosystems. Crop‐associated microbiomes play an important shaping performance, which includes but not limited to nutrient uptake, disease resistance, abiotic stress tolerance. Although our understanding structure function soil has been rapidly advancing, most knowledge comes from ecosystems natural environment. In this review, we present overview current communities along soil–plant continuum agroecosystems. To characterize mechanisms community assembly crop microbiomes, explore how host environmental factors such developmental stage, pathogen invasion, land management shape structure, co‐occurrence patterns, crop‐microbiome interactions. Particularly, relative importance deterministic stochastic is illustrated under different conditions, potential sources keystone taxa described. Finally, highlight few questions perspectives future research.

Language: Английский

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Microbiota-mediated nitrogen fixation and microhabitat homeostasis in aerial root-mucilage

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: April 21, 2023

Abstract Background Plants sustain intimate relationships with diverse microbes. It is well-recognized that these plant-associated microbiota shape individual performance and fitness of host plants, but much remains to be explored regarding how they exert their function maintain homeostasis. Results Here, using pink lady ( Heterotis rotundifolia ) as a study plant, we investigated the phenomenon microbiota-mediated nitrogen fixation elucidated this process steadily maintained in root mucilage microhabitat. Metabolite profiling showed aerial enriched carbohydrates diazotrophic bacteria. Nitrogen isotope-labeling experiments, 15 N natural abundance, gene expression analysis indicated root-mucilage could fix atmospheric support plant growth. While hotspot nutrients, did not observe high abundance other environmental pathogenic microbes inside. We further identified fungus isolate has shown broad-spectrum antimicrobial activities, solely allows growth This “friendly” may key driver Conclusion The discovery new biological mucilage-habitat friendly fungi provides insights into microbial homeostasis maintenance microenvironmental rhizosphere ecology.

Language: Английский

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Synergistic effects of diazotrophs and arbuscular mycorrhizal fungi on soil biological nitrogen fixation after three decades of fertilization

iMeta, Journal Year: 2023, Volume and Issue: 2(1)

Published: Jan. 27, 2023

Abstract Biological nitrogen (N) fixation (BNF) via diazotrophs is an important ecological process for the conversion of atmospheric N to biologically available N. Although soil play a dominant role in BNF and arbuscular mycorrhizal fungi (AMF) serve as helpers favor BNF, response diazotrophic communities different long‐term fertilizations AMF diazotrophs‐driven are poorly understood. Herein, 33‐year fertilization experiment wheat–maize intercropping system was conducted investigate changes rates, communities, their interactions after representative (chemical fertilizer, cow manure, wheat straw, green manure). We found remarkable increase rates more than three decades compared with nonfertilized soil, manure treatment rendered highest enhancement. The functionality strengthening mainly associated absolute abundance relative key cluster Module #0 (gained from co‐occurrence network species) such Skermanella Azospirillum . Furthermore, although positive correlations between were reduced under organic regimes, manuring could reverse decline within #0, this had relationship rate. This study suggests that promote select specific groups fixers certain areas. Our work provides solid evidence taxa interspecies will be largely favored fertilized strategy manure.

Language: Английский

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Dynamic metabolites: A bridge between plants and microbes

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 899, P. 165612 - 165612

Published: July 20, 2023

Language: Английский

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Concepts and consequences of the hyphosphere core microbiome for arbuscular mycorrhizal fungal fitness and function

New Phytologist, Journal Year: 2023, Volume and Issue: 242(4), P. 1529 - 1533

Published: Dec. 3, 2023

Summary Arbuscular mycorrhizal (AM) fungi‐associated hyphosphere microbiomes can be considered as the second genome of phosphorus uptake pathway. Their composition thought a stably recurring component holobiont, defined by core microbiome, which is to benefit AM fungal fitness. Here, we review evidence indicating existence highlight its functions linked those lacking in fungi, and further explore mechanisms different members ensure their stable coexistence. We conclude that deciphering utilizing microbiome provides an entry point for understanding complex interactions among plants, bacteria.

Language: Английский

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Foliar Applied ZnO Quantum Dots Boost Pumpkin (Cucurbita moschata Duch.) Growth and Positively Alter Endophytic and Rhizosphere Microbial Communities

ACS Sustainable Chemistry & Engineering, Journal Year: 2023, Volume and Issue: 11(23), P. 8503 - 8516

Published: May 30, 2023

Systematic understanding of the impact nanomaterials (NMs) on health and activity plant-associated microorganisms is required for sustainable development efficient nano-enabled agrochemicals. Zinc (Zn) an essential micronutrient plant growth development. It plays a vital role in various physiological processes, including enzyme activation, protein synthesis, cell division, etc. The present study investigated effects foliar exposure to different sizes ZnO particles, quantum dots (ZnO QDs, 4.06 nm), nanoparticles NPs, 60.0 bulk-scale particles BPs, 464.5 Zn ions at equivalent molar concentration (0.61 mM) endophytic rhizosphere microbial communities pumpkin seedlings. QDs increased total biomass by 24.6% as compared control. also contents chlorophyll carotenoids, elevated (Zn, Fe, B) uptake, triggered activities antioxidant enzymes, such peroxidase (POD), polyphenol oxidase (PPO), catalase (CAT), controls. composition diversity bacterial or fungal were significantly altered function NM size, significant difference between control treatments was evident. Linear discriminant effect size analysis (LEfSe) shows that putative beneficial host microbes Steroidobacter (relative abundance, RA: 1.05%) Paenibacillus (RA: 0.59%) enriched seedlings treated with which may lead greater growth, nutrient acquisition, stress resistance. Cooccurrence networks indicate both node link numbers 46.94 123.38% roots, could further stabilize community confer resistance environmental disturbance. Our overall findings demonstrate enhance directly improving performances indirectly modulating microorganisms. Thus, strategies can be promising approach increase crop stress.

Language: Английский

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