Nature Protocols, Journal Year: 2024, Volume and Issue: 19(9), P. 2712 - 2738
Published: May 3, 2024
Language: Английский
Nature Protocols, Journal Year: 2024, Volume and Issue: 19(9), P. 2712 - 2738
Published: May 3, 2024
Language: Английский
Nature Electronics, Journal Year: 2023, Volume and Issue: 6(12), P. 1032 - 1039
Published: Dec. 11, 2023
Language: Английский
Citations
110Nature Biotechnology, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 22, 2024
Language: Английский
Citations
56Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237
Published: March 5, 2024
Language: Английский
Citations
30Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 10, 2024
Abstract An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions human brain. To understand mechanistic significance projections on development circuits, we investigated an in vitro tissue model for connections, which two cerebral organoids are connected with a bundle reciprocally extended axons. The produced more intense oscillatory activity than conventional or directly fused organoids, suggesting inter-organoid axonal connections enhance support network activity. In addition, optogenetic stimulation axon bundles could entrain induce robust short-term plasticity macroscopic circuit. These results demonstrated projection axons serve as structural hub boosts functionality organoid-circuits. This contribute further investigation neuronal vitro.
Language: Английский
Citations
28Nature Neuroscience, Journal Year: 2024, Volume and Issue: 27(8), P. 1620 - 1631
Published: June 24, 2024
Language: Английский
Citations
20Cell stem cell, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
3Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115223 - 115223
Published: March 11, 2023
Language: Английский
Citations
41Nature reviews. Neuroscience, Journal Year: 2023, Volume and Issue: 25(1), P. 7 - 29
Published: Nov. 23, 2023
Language: Английский
Citations
36Trends in Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(8), P. 659 - 672
Published: June 22, 2023
Progression of Alzheimer's disease (AD) entails deterioration or aberrant function multiple brain cell types, eventually leading to neurodegeneration and cognitive decline. Defining how complex cell-cell interactions become dysregulated in AD requires novel human cell-based vitro platforms that could recapitulate the intricate cytoarchitecture diversity brain. Brain organoids (BOs) are 3D self-organizing tissues partially resemble architecture can AD-relevant pathology. In this review, we highlight versatile applications different types BOs model pathogenesis, including amyloid-β tau aggregation, neuroinflammation, myelin breakdown, vascular dysfunction, other phenotypes, as well accelerate therapeutic development for AD.
Language: Английский
Citations
34Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)
Published: Feb. 14, 2023
The bioengineerined and whole matured human brain organoids stand as highly valuable three-dimensional in vitro brain-mimetic models to recapitulate vivo development, neurodevelopmental neurodegenerative diseases. Various instructive signals affecting multiple biological processes including morphogenesis, developmental stages, cell fate transitions, migration, stem function immune responses have been employed for generation of physiologically functional cerebral organoids. However, the current approaches maturation require improvement harvestable with reduced batch-to-batch variabilities. Here, we demonstrate two different engineering approaches, rotating culture system (RCCS) microgravity bioreactor a newly designed microfluidic platform (µ-platform) improve harvestability, reproducibility survival high-quality compare those traditional spinner shaker systems. RCCS µ-platform reached ideal sizes, approximately 95% prolonged time Ki-67 + /CD31 /β-catenin+ proliferative, adhesive endothelial-like cells exhibited enriched cellular diversity (abundant neural/glial/ endothelial population), structural further neuronal identities (glutamate secreting glutamatergic, GABAergic hippocampal neurons) synaptogenesis (presynaptic-postsynaptic interaction) during development. Both expressed CD11b /IBA1 microglia MBP /OLIG2 oligodendrocytes at high levels day 60. showing physiological fidelity level can serve preclinical test new therapeutic regimens neurological diseases benefit from multiplexing.
Language: Английский
Citations
32