Generation of ‘semi-guided’ cortical organoids with complex neural oscillations DOI
Michael Q. Fitzgerald, Tiffany Chu, Francesca Puppo

et al.

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(9), P. 2712 - 2738

Published: May 3, 2024

Language: Английский

Brain organoid reservoir computing for artificial intelligence DOI
Hongwei Cai, Zheng Ao,

Chunhui Tian

et al.

Nature Electronics, Journal Year: 2023, Volume and Issue: 6(12), P. 1032 - 1039

Published: Dec. 11, 2023

Language: Английский

Citations

110

Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids DOI
Xiao Yang, Csaba Forró, Thomas L. Li

et al.

Nature Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 22, 2024

Language: Английский

Citations

56

Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications DOI
Sushila Maharjan,

Chenshuo Ma,

Bibhor Singh

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

Citations

30

Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons DOI Creative Commons
Tatsuya Osaki, Tomoya Duenki, Siu Yu A. Chow

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 10, 2024

Abstract An inter-regional cortical tract is one of the most fundamental architectural motifs that integrates neural circuits to orchestrate and generate complex functions human brain. To understand mechanistic significance projections on development circuits, we investigated an in vitro tissue model for connections, which two cerebral organoids are connected with a bundle reciprocally extended axons. The produced more intense oscillatory activity than conventional or directly fused organoids, suggesting inter-organoid axonal connections enhance support network activity. In addition, optogenetic stimulation axon bundles could entrain induce robust short-term plasticity macroscopic circuit. These results demonstrated projection axons serve as structural hub boosts functionality organoid-circuits. This contribute further investigation neuronal vitro.

Language: Английский

Citations

28

A high-density 1,024-channel probe for brain-wide recordings in non-human primates DOI
Yang Liu, Huilin Jia, Hongji Sun

et al.

Nature Neuroscience, Journal Year: 2024, Volume and Issue: 27(8), P. 1620 - 1631

Published: June 24, 2024

Language: Английский

Citations

20

Vascular network-inspired diffusible scaffolds for engineering functional midbrain organoids DOI
Hongwei Cai,

Chunhui Tian,

Lei Chen

et al.

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

3

A mesh microelectrode array for non-invasive electrophysiology within neural organoids DOI
Matthew G. McDonald, David Sebinger,

Lisa Brauns

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115223 - 115223

Published: March 11, 2023

Language: Английский

Citations

41

Human neuronal maturation comes of age: cellular mechanisms and species differences DOI
Jenelle L. Wallace, Alex A. Pollen

Nature reviews. Neuroscience, Journal Year: 2023, Volume and Issue: 25(1), P. 7 - 29

Published: Nov. 23, 2023

Language: Английский

Citations

36

Pushing the boundaries of brain organoids to study Alzheimer’s disease DOI Creative Commons

Jonas Cerneckis,

Guojun Bu, Yanhong Shi

et al.

Trends in Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(8), P. 659 - 672

Published: June 22, 2023

Progression of Alzheimer's disease (AD) entails deterioration or aberrant function multiple brain cell types, eventually leading to neurodegeneration and cognitive decline. Defining how complex cell-cell interactions become dysregulated in AD requires novel human cell-based vitro platforms that could recapitulate the intricate cytoarchitecture diversity brain. Brain organoids (BOs) are 3D self-organizing tissues partially resemble architecture can AD-relevant pathology. In this review, we highlight versatile applications different types BOs model pathogenesis, including amyloid-β tau aggregation, neuroinflammation, myelin breakdown, vascular dysfunction, other phenotypes, as well accelerate therapeutic development for AD.

Language: Английский

Citations

34

Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids DOI Creative Commons
Pelin Sağlam-Metiner, Utku Devamoglu, Yagmur Filiz

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Feb. 14, 2023

The bioengineerined and whole matured human brain organoids stand as highly valuable three-dimensional in vitro brain-mimetic models to recapitulate vivo development, neurodevelopmental neurodegenerative diseases. Various instructive signals affecting multiple biological processes including morphogenesis, developmental stages, cell fate transitions, migration, stem function immune responses have been employed for generation of physiologically functional cerebral organoids. However, the current approaches maturation require improvement harvestable with reduced batch-to-batch variabilities. Here, we demonstrate two different engineering approaches, rotating culture system (RCCS) microgravity bioreactor a newly designed microfluidic platform (µ-platform) improve harvestability, reproducibility survival high-quality compare those traditional spinner shaker systems. RCCS µ-platform reached ideal sizes, approximately 95% prolonged time Ki-67 + /CD31 /β-catenin+ proliferative, adhesive endothelial-like cells exhibited enriched cellular diversity (abundant neural/glial/ endothelial population), structural further neuronal identities (glutamate secreting glutamatergic, GABAergic hippocampal neurons) synaptogenesis (presynaptic-postsynaptic interaction) during development. Both expressed CD11b /IBA1 microglia MBP /OLIG2 oligodendrocytes at high levels day 60. showing physiological fidelity level can serve preclinical test new therapeutic regimens neurological diseases benefit from multiplexing.

Language: Английский

Citations

32