Multifunctional Conductive and Electrogenic Hydrogel Repaired Spinal Cord Injury via Immunoregulation and Enhancement of Neuronal Differentiation

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)

Published: Feb. 3, 2024

Abstract Spinal cord injury (SCI) is a refractory neurological disorder. Due to the complex pathological processes, especially secondary inflammatory cascade and lack of intrinsic regenerative capacity, it difficult recover function after SCI. Meanwhile, simulating conductive microenvironment spinal reconstructs electrical neural signal transmission interrupted by SCI facilitates repair. Therefore, double‐crosslinked hydrogel (BP@Hydrogel) containing black phosphorus nanoplates (BP) synthesized. When placed in rotating magnetic field (RMF), BP@Hydrogel can generate stable signals exhibit electrogenic characteristic. In vitro, shows satisfactory biocompatibility alleviate activation microglia. RMF, enhances anti‐inflammatory effects. wireless stimulation promotes differentiation stem cells (NSCs) into neurons, which associated with PI3K/AKT pathway. vivo, injectable elicit behavioral electrophysiological recovery complete transected mice alleviating inflammation facilitating endogenous NSCs form functional neurons synapses under RMF. The present research develops multifunctional for repair targeting multiple mechanisms including immunoregulation enhancement neuronal differentiation.

Language: Английский

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Sea Cucumber-Inspired Microneedle Nerve Guidance Conduit for Synergistically Inhibiting Muscle Atrophy and Promoting Nerve Regeneration

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14427 - 14440

Published: May 22, 2024

Muscle atrophy resulting from peripheral nerve injury (PNI) poses a threat to patient's mobility and sensitivity. However, an effective method inhibit muscle following PNI remains elusive. Drawing inspiration the sea cucumber, we have integrated microneedles (MNs) microchannel technology into guidance conduits (NGCs) develop bionic microneedle NGCs (MNGCs) that emulate structure piezoelectric function of cucumbers. Morphologically, MNGCs feature outer surface with outward-pointing needle tips capable applying electrical stimulation denervated muscles. Simultaneously, interior contains microchannels designed guide migration Schwann cells (SCs). Physiologically, incorporation conductive reduced graphene oxide zinc nanoparticles polycaprolactone scaffold enhances conductivity properties, facilitating SCs' migration, myelin regeneration, axon growth, restoration neuromuscular function. These combined effects ultimately lead inhibition Consequently, concept synergistic effect inhibiting promoting regeneration has capacity transform traditional approach repair find broad applications in repair.

Language: Английский

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Microneedles as Transdermal Drug Delivery System for Enhancing Skin Disease Treatment

Acta Pharmaceutica Sinica B, Journal Year: 2024, Volume and Issue: 14(12), P. 5161 - 5180

Published: Aug. 23, 2024

Microneedles (MNs) serve as a revolutionary paradigm in transdermal drug delivery, heralding viable resolution to the formidable barriers presented by cutaneous interface. This review examines MNs an advanced approach enhancing dermatological pathology management. It explores complex dermis structure and highlights limitations of traditional methods, emphasizing MNs' advantage bypassing stratum corneum deliver drugs directly subdermal matrix. The discourse outlines diverse typologies MNs, including solid, coated, hollow, hydrogel, dissolvable versions. Each type is characterized its unique applications benefits. treatise details deployment alleviation cancers, administration inflammatory dermatoses such psoriasis atopic dermatitis, their utility wound Additionally, paper contemplates prospects within realm aesthetic dermatology burgeoning market traction cosmetic MN formulations. summarizes scientific commercial challenges clinical adoption therapeutics, dosage calibration, pharmacodynamics, biocompatibility, patient compliance, sterilization, mass production, regulatory oversight. emphasizes need for ongoing research, innovation, harmonization overcome these obstacles fully realize potential treating skin diseases improving welfare. presents comprehensive overview recent accomplishments, remaining challenges, future microneedle-facilitated therapy afflictions.

Language: Английский

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Frozen reinforced microneedles loaded with NIR-photothermal nanozyme for keratitis treatment

Nano Today, Journal Year: 2023, Volume and Issue: 52, P. 102000 - 102000

Published: Sept. 23, 2023

Language: Английский

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Microneedle‐Mediated Cell Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 11(8)

Published: Oct. 30, 2023

Microneedles have emerged as a promising platform for transdermal drug delivery with prominent advantages, such enhanced permeability, mitigated pain, and improved patient adherence. While microneedles primarily been employed delivering small molecules, nucleic acids, peptides, proteins, recent researches demonstrated their prospect in combination cell therapy. Cell therapy involving administration or transplantation of living cells (e.g. T cells, stem pancreatic cells) has gained significant attention preclinical clinical applications various disease treatments. However, the effectiveness systemic may be restricted localized conditions like solid tumors skin disorders due to limited penetration accumulation into lesions. In this perspective, an overview advances microneedle-assisted immunotherapy, tissue regeneration, hormone modulation, respect mechanical property, loading capacity, well viability bioactivity loaded is provided. Potential challenges future perspectives microneedle-mediated are also discussed.

Language: Английский

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A differential-targeting core–shell microneedle patch with coordinated and prolonged release of mangiferin and MSC-derived exosomes for scarless skin regeneration

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(11), P. 2667 - 2684

Published: Jan. 1, 2024

Microneedles for skin regeneration are conventionally restricted by uncontrollable multi-drug release, limited types of drugs, and poor wound adhesion. Here, a novel core-shell microneedle patch is developed scarless repair, where the shell composed hydrophilic gelatin methacryloyl (GelMA) loaded with mangiferin, an anti-inflammatory small molecule, core hydrophobic poly (lactide-co-propylene glycol-co-lactide) dimethacrylates (PGLADMA) bioactive macromolecule human mesenchymal stromal cell (hMSC)-derived exosomes. This material choice provides several benefits: GelMA swelling interface tissue interlocking rapid release mangiferin at early healing stage anti-inflammation, whereas PGLADMA offers long-term encapsulation exosomes (30% in 3 weeks), promoting sustained angiogenesis anti-inflammation. Our results demonstrate that possesses properties can induce both vitro terms macrophage polarization tube formation umbilical vein endothelial cells (HUVECs), vivo re-epithelization, vessel formation. Importantly, we also observe reduced scar vivo. Altogether, degradation dynamics our hydrophilic/hydrophobic materials enable design differential prolonged regeneration, potential other therapies exosome release.

Language: Английский

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Dual engine-driven bionic microneedles for early intervention and prolonged treatment of Alzheimer's disease

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 367, P. 184 - 196

Published: Jan. 26, 2024

Language: Английский

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Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

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Wearable and implantable biosensors: mechanisms and applications in closed-loop therapeutic systems

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(35), P. 8577 - 8604

Published: Jan. 1, 2024

Biosensors integrated into closed-loop systems provide patient-specific adaptive therapies. These advanced devices have significant potential for managing chronic conditions by continuously adjusting therapeutic regimens based on physiological data.

Language: Английский

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Microorganism microneedle micro-engine depth drug delivery

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 17, 2024

As a transdermal drug delivery method, microneedles offer minimal invasiveness, painlessness, and precise in-situ treatment. However, current rely on passive diffusion, leading to uncontrollable penetration. To overcome this, we developed pneumatic microneedle patch that uses live Enterobacter aerogenes as microengines actively control delivery. These microbes generate gas, driving drugs into deeper tissues, with adjustable glucose concentration allowing over the process. Our results showed this microorganism-powered system increases depth by 200%, reaching up 1000 μm below skin. In psoriasis animal model, technology effectively delivered calcitriol subcutaneous offering rapid symptom relief. This innovation addresses limitations of conventional microneedles, enhancing efficiency, permeability, introducing creative paradigm for on-demand controlled

Language: Английский

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