Advancements and Future Prospects of CRISPR-Cas-Based Population Replacement Strategies in Insect Pest Management DOI Creative Commons
Yu Zhao, L.-L. Li,

Liangzi Wei

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 653 - 653

Published: Aug. 30, 2024

Population replacement refers to the process by which a wild-type population of insect pests is replaced possessing modified traits or abilities. Effective necessitates gene drive system capable spreading desired genes within natural populations, operating under principles akin super-Mendelian inheritance. Consequently, releasing small number genetically edited insects could potentially achieve control objectives. Currently, several approaches are exploration, including newly adapted CRISPR-Cas genome editing system. Multiple studies investigating methods engineer that incapable causing crop damage transmitting vector-borne diseases, with notable successful examples documented. This review summarizes recent advancements in realm and provides insights into research methodologies, testing protocols, implementation strategies for techniques. The also discusses emerging trends prospects establishing genetic tools pest management.

Language: Английский

Curing mosquitoes with genetic approaches for malaria control DOI
Mary Kefi, Víctor Cardoso-Jaime, Sally A. Saab

et al.

Trends in Parasitology, Journal Year: 2024, Volume and Issue: 40(6), P. 487 - 499

Published: May 16, 2024

Language: Английский

Citations

10
Cleave and Rescue gamete killers create conditions for gene drive in plants DOI
Georg Oberhofer, Michelle L. Johnson, Tobin Ivy

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: 10(6), P. 936 - 953

Published: June 17, 2024

Language: Английский

Citations

8
Considerations for first field trials of low-threshold gene drive for malaria vector control DOI Creative Commons
John B. Connolly, Austin Burt, George K. Christophides

et al.

Malaria Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: May 21, 2024

Sustainable reductions in African malaria transmission require innovative tools for mosquito control. One proposal involves the use of low-threshold gene drive Anopheles vector species, where a 'causal pathway' would be initiated by (i) release system target leading to (ii) its subsequent generations, (iii) increase frequency and spread populations, (iv) simultaneous propagation linked genetic trait aimed at reducing vectorial capacity Plasmodium, (v) reduced parasites populations as reaches fixation causing (vi) decreased incidence prevalence. Here scope, objectives, trial design elements, approaches monitoring initial field releases such dive systems are considered, informed successful implementation trials biological control agents, well other tools, including insecticides, Wolbachia, larvicides, attractive-toxic sugar bait systems. Specific research questions addressed identified, adaptive is explored potentially constructive flexible approach facilitate testing causal pathway. A fundamental question decision-makers first will whether there should selective focus on earlier points pathway, efficacy via measurement or wider interrogation entire pathway entomological epidemiological efficacy. How when eventually assessed an essential consideration before decisions any protocols finalized implemented, regardless exclusively efficacy, broader aspects Statistical modelling currently under active development inform design, locations, endpoints. Collectively, considerations here advance realization developer ambitions within next 5 years.

Language: Английский

Citations

6
Integrating malaria vaccine and CRISPR/Cas9 gene drive: a comprehensive strategy for accelerated malaria eradication DOI Creative Commons
Israel Charles Abraham, John Ehi Aboje, Bonaventure Michael Ukoaka

et al.

Malaria Journal, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 17, 2025

Malaria remains a significant public health challenge, particularly in low- and middle-income countries, despite ongoing efforts to eradicate the disease. Recent advancements, including rollout of malaria vaccines, such as RTS,S/AS01 R21/Matrix-M™, offer new avenues for prevention. However, rise resistance anti-malarial medications necessitates innovative strategies. This review explores potential integration CRISPR/Cas9 gene drive technology with vaccination enhance vector control reduce transmission. By employing mechanisms population suppression replacement malaria-transmitting Anopheles mosquitoes, combined immunogenic properties synergistic approach can be established. paper discussed need integrated strategies address biological complexities socio-economic factors influencing its prevalence. Challenges regulatory hurdles, community acceptance, ecological impacts, sustainable funding are examined, alongside implementation within existing programmes. could significantly contribute achieving World Health Organization's targets reduction by 2030, ultimately enhancing outcomes supporting broader development.

Language: Английский

Citations

0
Full title: Synthetic Homing Endonuclease Gene Drives to Revolutionise Aedes aegypti Biocontrol - Game Changer or Pipe Dream? DOI
Joshua X. D. Ang, Sebald A. N. Verkuijl, Michelle A. E. Anderson

et al.

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101373 - 101373

Published: April 1, 2025

Language: Английский

Citations

0
The Perpetual Vector Mosquito Threat and Its Eco-Friendly Nemeses DOI Creative Commons

Leticia Silva Miranda,

Sarah Renee Rudd,

Oscar C. Mena

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(3), P. 182 - 182

Published: March 12, 2024

Mosquitoes are the most notorious arthropod vectors of viral and parasitic diseases for which approximately half world’s population, ~4,000,000,000, is at risk. Integrated pest management programs (IPMPs) have achieved some success in mitigating regional transmission persistence these diseases. However, as many vector-borne remain pervasive, it obvious that IPMP successes not been absolute eradicating threat imposed by mosquitoes. Moreover, expanding mosquito geographic ranges caused factors related to climate change globalization (travel, trade, migration), evolution resistance synthetic pesticides, present ongoing challenges reducing or eliminating local global burden diseases, especially economically medically disadvantaged societies. Abatement strategies include control vector populations with pesticides eco-friendly technologies. These “green” technologies SIT, IIT, RIDL, CRISPR/Cas9 gene drive, biological specifically targets aquatic larval stages Regarding latter, effective continues be widespread use Lysinibacillus sphaericus (Ls) Bacillus thuringiensis subsp. israelensis (Bti). Here, we a review health issues elicited mosquitoes, strategies, lastly, focus on biology Ls Bti, an emphasis no has observed field.

Language: Английский

Citations

3
Gene drives: an alternative approach to malaria control? DOI Creative Commons
Kubendran Naidoo, Shüné V. Oliver

Gene Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: July 22, 2024

Abstract Genetic modification for the control of mosquitoes is frequently touted as a solution variety vector-borne diseases. There has been some success using non-insecticidal methods like sterile or incompatible insect techniques to arbovirus However, by genetic modifications reduce mosquito populations create that are refractory infection with pathogens less developed. The advent CRISPR-Cas9-mediated gene drives may advance this mechanism control. In review, use and progress vector control, particularly malaria, discussed. A brief history population suppression replacement in mosquitoes, rapid advancement field over last decade how fits into current scope described. Mechanisms alternative modulate mosquitoes’ immune responses anti-parasite effector molecules part combinational strategy combat malaria considered. Finally, limitations ethics

Language: Английский

Citations

2
Versatile plant genome engineering using anti-CRISPR-Cas12a systems DOI
Yao He, Shishi Liu, Long Chen

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

Language: Английский

Citations

2
Cleave and Rescue gamete killers create conditions for gene drive in plants DOI Creative Commons
Georg Oberhofer, Michelle L. Johnson, Tobin Ivy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 14, 2023

Abstract Gene drive elements promote the spread of linked traits, even when their presence confers a fitness cost to carriers, and can be used change composition or fate wild populations. Cleave Rescue ( ClvR ) sit at fixed chromosomal position include DNA sequence-modifying enzyme such as Cas9/gRNAs (the Cleaver/Toxin) that disrupts endogenous versions an essential gene, recoded version gene resistant cleavage Rescue/Antidote). spreads by creating conditions in which those lacking die because they lack functional gene. We demonstrate features plant Arabidopsis thaliana through killing gametes fail inherit targets YKT61, whose expression is required male female for survival. Resistant (uncleavable but functional) alleles, slow prevent drive, were not observed. Modeling shows s are likely robust certain failure modes rapidly population modification suppression. Possible applications breeding, weed control, conservation discussed.

Language: Английский

Citations

4
Advancements and Future Prospects of CRISPR-Cas-Based Population Replacement Strategies in Insect Pest Management DOI Creative Commons
Yu Zhao, L.-L. Li,

Liangzi Wei

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 653 - 653

Published: Aug. 30, 2024

Population replacement refers to the process by which a wild-type population of insect pests is replaced possessing modified traits or abilities. Effective necessitates gene drive system capable spreading desired genes within natural populations, operating under principles akin super-Mendelian inheritance. Consequently, releasing small number genetically edited insects could potentially achieve control objectives. Currently, several approaches are exploration, including newly adapted CRISPR-Cas genome editing system. Multiple studies investigating methods engineer that incapable causing crop damage transmitting vector-borne diseases, with notable successful examples documented. This review summarizes recent advancements in realm and provides insights into research methodologies, testing protocols, implementation strategies for techniques. The also discusses emerging trends prospects establishing genetic tools pest management.

Language: Английский

Citations

1