Technologies and Standardization in Research on Extracellular Vesicles DOI Creative Commons
Srujan Gandham, Xianyi Su, Jacqueline Wood

et al.

Trends in biotechnology, Journal Year: 2020, Volume and Issue: 38(10), P. 1066 - 1098

Published: June 18, 2020

Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component variety pathological processes. Potential applications EVs in novel diagnostic therapeutic strategies have brought increasing attention. However, EV research remains highly challenging due to the inherently complex biogenesis their vast heterogeneity size, composition, origin. There need for establishment standardized methods that address sources pre-analytical analytical variability studies. Here, we review technologies developed isolation characterization discuss paths toward standardization research.

Language: Английский

The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles DOI
Andrew M. Leidal, Hector H. Huang, Timothy Marsh

et al.

Nature Cell Biology, Journal Year: 2020, Volume and Issue: 22(2), P. 187 - 199

Published: Jan. 13, 2020

Language: Английский

Citations

391
Plant Exosome-like Nanovesicles: Emerging Therapeutics and Drug Delivery Nanoplatforms DOI Creative Commons
Haseeb Anwar Dad,

Ting-Wei Gu,

Ao-Qing Zhu

et al.

Molecular Therapy, Journal Year: 2020, Volume and Issue: 29(1), P. 13 - 31

Published: Dec. 3, 2020

Plant exosome-like nanovesicles, being innately replete with bioactive lipids, proteins, RNA, and other pharmacologically active molecules, offer unique morphological compositional characteristics as natural nanocarriers. Furthermore, their compelling physicochemical traits underpin modulative role in physiological processes, all of which have fostered the concept that these nanovesicles may be highly proficient development next-generation biotherapeutic drug delivery nanoplatforms to meet ever-stringent demands current clinical challenges. This review systemically deals various facets plant ranging from origin isolation identification composition, biological functions, cargo-loading mechanisms. Efforts are made encompass roles by elucidating immunological modulating, anti-tumor, regenerative, anti-inflammatory roles. We also shed light on re-engineering into robust, innocuous, non-immunogenic nanovectors for through multiple stringent hindrances targeted organs such intestine brain. Finally, recent advances centered around along new insights transdermal, transmembrane targeting mechanisms vesicles elucidated. expect continuing nanovesicle-based therapeutic will promote applications.

Language: Английский

Citations

386
Exosomes: Large-scale production, isolation, drug loading efficiency, and biodistribution and uptake DOI
Ilgin Kimiz‐Gebologlu, Suphi Ş. Öncel

Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 347, P. 533 - 543

Published: May 24, 2022

Language: Английский

Citations

376
Context-specific regulation of extracellular vesicle biogenesis and cargo selection DOI
Andrew C. Dixson,

T. Renee Dawson,

Dolores Di Vizio

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 454 - 476

Published: Feb. 10, 2023

Language: Английский

Citations

376
Technologies and Standardization in Research on Extracellular Vesicles DOI Creative Commons
Srujan Gandham, Xianyi Su, Jacqueline Wood

et al.

Trends in biotechnology, Journal Year: 2020, Volume and Issue: 38(10), P. 1066 - 1098

Published: June 18, 2020

Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component variety pathological processes. Potential applications EVs in novel diagnostic therapeutic strategies have brought increasing attention. However, EV research remains highly challenging due to the inherently complex biogenesis their vast heterogeneity size, composition, origin. There need for establishment standardized methods that address sources pre-analytical analytical variability studies. Here, we review technologies developed isolation characterization discuss paths toward standardization research.

Language: Английский

Citations

369