RNA delivery by extracellular vesicles in mammalian cells and its applications

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(10), P. 585 - 606

Published: May 26, 2020

Language: Английский

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Challenges and directions in studying cell–cell communication by extracellular vesicles

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(5), P. 369 - 382

Published: March 8, 2022

Language: Английский

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Autophagy and autophagy-related pathways in cancer

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(8), P. 560 - 575

Published: March 2, 2023

Language: Английский

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Autophagy in inflammation, infection, and immunometabolism

Immunity, Journal Year: 2021, Volume and Issue: 54(3), P. 437 - 453

Published: March 1, 2021

Language: Английский

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Context-specific regulation of extracellular vesicle biogenesis and cargo selection

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 454 - 476

Published: Feb. 10, 2023

Language: Английский

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Extracellular vesicles and nanoparticles: emerging complexities

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 33(8), P. 667 - 681

Published: Feb. 1, 2023

The study of extracellular vesicles (EVs) and nanoparticles (NPs) is rapidly expanding because recent discoveries have revealed a much greater complexity diversity than was appreciated only few years ago. New types EVs NPs recently been described. Proteins nucleic acids previously thought to be packaged in exosomes appear more enriched different two identified amembranous NPs, exomeres supermeres. Thus, our understanding the cell biology intercellular communication facilitated by release state flux. In this review, we describe highlight advances, present major outstanding questions.

Language: Английский

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Autophagy genes in biology and disease

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 24(6), P. 382 - 400

Published: Jan. 12, 2023

Language: Английский

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Exosome biogenesis: machinery, regulation, and therapeutic implications in cancer

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Nov. 1, 2022

Abstract Exosomes are well-known key mediators of intercellular communication and contribute to various physiological pathological processes. Their biogenesis involves four steps, including cargo sorting, MVB formation maturation, transport MVBs, fusion with the plasma membrane. Each process is modulated through competition or coordination multiple mechanisms, whereby diverse repertoires molecular cargos sorted into distinct subpopulations exosomes, resulting in high heterogeneity exosomes. Intriguingly, cancer cells exploit strategies, such as aberrant gene expression, posttranslational modifications, altered signaling pathways, regulate biogenesis, composition, eventually functions exosomes promote progression. Therefore, exosome biogenesis-targeted therapy being actively explored. In this review, we systematically summarize recent progress understanding machinery how it regulated context cancer. particular, highlight pharmacological targeting a promising therapeutic strategy.

Language: Английский

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Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering

Advanced Science, Journal Year: 2020, Volume and Issue: 8(1)

Published: Nov. 27, 2020

Abstract Extracellular vesicles (EVs) are biocompatible, nano‐sized secreted containing many types of biomolecules, including proteins, RNAs, DNAs, lipids, and metabolites. Their low immunogenicity ability to functionally modify recipient cells by transferring diverse bioactive constituents make them an excellent candidate for a next‐generation drug delivery system. Here, the recent advances in EV biology emerging strategies bioengineering summarized, prospects clinical translation bioengineered EVs challenges be overcome discussed.

Language: Английский

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Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Cells, Journal Year: 2020, Volume and Issue: 9(9), P. 1955 - 1955

Published: Aug. 25, 2020

Research on extracellular vesicles (EVs) is growing exponentially due to an increasing appreciation of EVs as disease biomarkers and therapeutics, expanding number EV-containing materials under study, application new preparation, detection, cargo analysis methods. Diversity both sources methodologies imposes challenges the comparison measurement results between studies laboratories. While reference guidelines minimal requirements for EV research have achieved important objective assembling community consensus, it also essential understand which quality controls are currently being applied, how usage trends evolving. As initial response this need, International Society Extracellular Vesicles (ISEV) performed a worldwide survey in 2015 "Techniques used isolation characterization vesicles" published from 2016. In 2019, was assess changing state field. The questionnaire received more than 600 full or partial responses, present manuscript summarizes second survey. emphasize that separation methods such ultracentrifugation density gradients still most commonly methods, use size exclusion chromatography has increased, techniques based tangential flow microfluidics now by 10% respondents. reveals researchers do not perform sample before after EVs. Finally, majority should receive attention.

Language: Английский

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