Host Specificity of Snodgrassella in Eastern and Western Honeybees and Its Effects on Naturally Occurring Deformed Wing Virus Titers DOI Creative Commons

Nihong Zhou,

Shuo Yang, Ruike Wei

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 478 - 478

Published: May 1, 2025

Honeybee gut microbiota undergoes long-term adaptive evolution with the host, resulting in host-specific genomic and functional characteristics. However, specific role of host-specialized strains shaping host physiological functions remains understudied. This study investigates specificity core symbiont Snodgrassella A. cerana mellifera, exploring its effects on immune response natural virus suppression through analysis colonization experiments. Genomic revealed that strain from exhibited a larger genome greater gene content compared to cerana. Competitive experiments showed although different origins had similar efficiency displayed clear home-field advantage competitive process. Moreover, Inoculation mellifera native significantly reduced Deformed Wing Virus (DWV) titers, whereas non-native no effect. In contrast, neither altered DWV or Sacbrood (SBV) levels Immune only upregulated defensin 2 while significant changes occurred either strain. These results suggest exhibits at level, influencing both suppression, showing efficacy these roles, especially mellifera.

Language: Английский

The honeybee microbiota and its impact on health and disease DOI
Erick V. S. Motta, Nancy A. Moran

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(3), P. 122 - 137

Published: Dec. 4, 2023

Language: Английский

Citations

67
Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi DOI Creative Commons
Andrew Quinn, Yassine El Chazli, Stéphane Escrig

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(2), P. 477 - 489

Published: Jan. 15, 2024

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about role of host-derived enabling bacterial colonization. Here we examined metabolic interactions within evolutionary ancient symbiosis between honey bee (Apis mellifera) and core microbiota member Snodgrassella alvi. This betaproteobacterium incapable metabolizing saccharides, yet colonizes presence a sugar-only diet. Using comparative metabolomics,

Language: Английский

Citations

22
Ecotoxicological effects of common fungicides on the eastern honeybee Apis cerana cerana (Hymenoptera) DOI Creative Commons
Minjie Huang, Jie Dong, Shuyuan Yang

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 868, P. 161637 - 161637

Published: Jan. 14, 2023

The widespread use of fungicides for plant protection has increased the potential pollinator exposure. This study therefore aimed at assessing acute and chronic effects on pollinators. For this purpose, oral toxicity common azoxystrobin, pyraclostrobin, boscalid to Eastern honeybee Apis cerana cerena was first evaluated, multiple aspects were investigated after exposure a one-tenth medium lethal dose (LD50) 10 days. revealed that LD50 values pyraclostrobin adult honeybees 12.7 μg/bee, 36.6 >119 respectively. Midgut epithelial cells fungicide caused intercellular spaces varying degrees vacuolization. Exposure these three their binary mixtures significantly affected glycerophospholipid, alanine, aspartate, glutamate metabolism in midguts. Additionally, relative composition Lactobacillus, dominant functional genus guts decreased microbial balance disrupted. All induced strong transcriptional upregulation genes associated with immune response encoding enzymes related oxidative phosphorylation metabolism, including abaecin, apidaecin, hymenotaecin, cyp4c3, cyp6a2 hbg3. Our provides important insight understanding commonly used nontarget contributes more comprehensive assessment ecological environmental safety.

Language: Английский

Citations

29
Gut microbiota contribute to variations in honey bee foraging intensity DOI Creative Commons
Cassondra Vernier,

Lan Anh Nguyen,

Tim Gernat

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Gut microbiomes are increasingly recognized for mediating diverse biological aspects of their hosts, including complex behavioral phenotypes. Although many studies have reported that experimental disruptions to the gut microbial community result in atypical host behavior, address how microbes contribute adaptive trait variation rare. Eusocial insects represent a powerful model test this, because simple microbiota and division labor characterized by colony-level previous report correlational differences associated with labor, here, we provide evidence play causal role defining foraging behavior between European honey bees (Apis mellifera). We found structure differed hive-based nurse leave hive forage floral resources. These were abundance individual microbes, Bifidobacterium asteroides, Bombilactobacillus mellis, Lactobacillus melliventris. Manipulations colony demography experience suggested composition task experience. Moreover, single-microbe inoculations B. L. melliventris caused effects on intensity. results demonstrate social insect, support modulating variation.

Language: Английский

Citations

11
Insects' essential role in understanding and broadening animal medication DOI Creative Commons
Silvio Erler, Sheena C. Cotter, Dalial Freitak

et al.

Trends in Parasitology, Journal Year: 2024, Volume and Issue: 40(4), P. 338 - 349

Published: March 4, 2024

Like humans, animals use plants and other materials as medication against parasites. Recent decades have shown that the study of insects can greatly advance our understanding behaviors. The ease rearing under laboratory conditions has enabled controlled experiments to test critical hypotheses, while their spectrum reproductive strategies living arrangements – ranging from solitary eusocial communities revealed behaviors evolve maximize inclusive fitness through both direct indirect benefits. Studying also demonstrated in some cases act modulation host's innate immune system microbiome. We highlight outstanding questions, focusing on costs benefits context host fitness.

Language: Английский

Citations

10
Strain diversity and host specificity of the gut symbiont Gilliamella in Apis mellifera, Apis cerana and Bombus terrestris DOI

Nihong Zhou,

Qi Zheng,

Yao Liu

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128048 - 128048

Published: Jan. 6, 2025

Language: Английский

Citations

1
Deep Divergence and Genomic Diversification of Gut Symbionts of Neotropical Stingless Bees DOI Creative Commons
Garance Sarton-Lohéac, Carlos Gustavo Nunes-Silva, Florent Mazel

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: March 20, 2023

Social bees harbor conserved gut microbiotas that may have been acquired in a common ancestor of social and subsequently codiversified with their hosts. However, most this knowledge is based on studies the honey bumblebees. Much less known about third diverse group bees, stingless bees. Specifically, absence genomic data from presents an important gap understanding evolution functional diversity bee microbiota. Here, we combined community profiling culturing genome sequencing bacteria six neotropical species Brazil. Phylogenomic analyses show isolates form deep-branching sister clades core members bumblebee microbiota capabilities, confirming ancestry ecology our bacterial phylogenies were not congruent those host, indicating was driven by strict codiversification but included host switches independent symbiont gain losses. Finally, as reported for microbiotas, found substantial divergence among strains bacteria, suggesting adaptation to different glycan niches. Our study offers first insights into highlights need broader samplings understand IMPORTANCE Stingless are corbiculate represent pollinator throughout tropics subtropics. They specialized microbial communities related bumblebees likely health. Few cultured which has prevented characterization potential. established cultures major sequenced genomes. We belong novel distantly encoding similar capabilities. new perspective basis characterizing symbiotic relationships between

Language: Английский

Citations

20
Gut microbiota influences onset of foraging-related behavior but not physiological hallmarks of division of labor in honeybees DOI Creative Commons
Joanito Liberti, Erik T. Frank, Tomas Kay

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: July 29, 2024

ABSTRACT Gut microbes can impact cognition and behavior, but whether they regulate the division of labor in animal societies is unknown. We addressed this question using honeybees since exhibit between nurses foragers because their gut microbiota be manipulated. Using automated behavioral tracking controlling for co-housing effects, we show that influence age at which bees start expressing foraging-like behaviors laboratory have no effects on time spent a foraging arena number trips. Moreover, did not hallmarks maturation such as body weight, cuticular hydrocarbon profile, hypopharyngeal gland size, gene expression, proportion maturing into foragers. Overall, study shows honeybee plays role onset foraging-related behavior without permanent consequences colony-level several physiological maturation. IMPORTANCE The emerging model system studying microbiota-host interactions. Previous studies reported multiple worker bee phenotypes, all change during maturation—the transition from nursing to foraging. tested documented may stem an effect only subtly affected maturation: it accelerated affecting overall or average output. also found host (CHC) expression maturation-related genes. These results are inconsistent with previous reporting weight CHC profile. Our experiments revealed co-housed tend converge physiology, suggesting spurious associations emerge when rearing environments replicated sufficiently accounted analytically.

Language: Английский

Citations

8
Synergistic resistance of honeybee (Apis mellifera) and their gut microorganisms to fluvalinate stress DOI
Jianhui Liu,

Chunhua Liao,

Zhen Li

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 201, P. 105865 - 105865

Published: March 12, 2024

Language: Английский

Citations

6
The honey bee “hive” microbiota: meta-analysis reveals a native and aerobic microbiota prevalent throughout the social resource niche DOI Creative Commons
Kirk E. Anderson,

Duan Copeland

Frontiers in Bee Science, Journal Year: 2024, Volume and Issue: 2

Published: July 5, 2024

The microbiome of the honey bee worker hindgut has been explored thoroughly with culturing and next-generation sequencing revealing both composition function. However, less effort devoted to aerobic social niches associated hive environment colony process. We performed a meta-analysis 3,800+ publicly available 16S rRNA gene sequence libraries examining hypothesis native microbiota interaction resources. selected high-throughput studies represent tissue-specific samples, including nine distinct throughout hive, defined by nutrient processing. These included queen gut tissues, foregut, midgut, ileum, rectum, mouthparts, glands, developing larvae, secreted stored nutrition. found that foregut midgut queens workers share significant portion their larval rearing secretion storage, defining resource niche. Characterized species dominance rapid growth, functions primarily in disease prevention at individual level may also function communication resilience. Defining contributes systems-level understanding host–microbial interactions bee.

Language: Английский

Citations

6