Nature reviews. Immunology, Journal Year: 2023, Volume and Issue: 24(1), P. 18 - 32
Published: July 4, 2023
Language: Английский
Cell, Journal Year: 2024, Volume and Issue: 187(7), P. 1589 - 1616
Published: March 1, 2024
Language: Английский
Citations
173
Seminars in Cancer Biology, Journal Year: 2023, Volume and Issue: 89, P. 61 - 75
Published: Jan. 20, 2023
Language: Английский
Citations
151
Cells, Journal Year: 2023, Volume and Issue: 12(7), P. 1001 - 1001
Published: March 24, 2023
Spheroids and organoids are important novel players in medical life science research. They gradually replacing two-dimensional (2D) cell cultures. Indeed, three-dimensional (3D) cultures closer to the vivo reality open promising perspectives for academic research, drug screening, personalized medicine. A large variety of cells tissues, including tumor cells, can be starting material generation 3D cultures, primary stem or lines. panoply methods has been developed generate structures, spontaneous forced aggregation, air–liquid interface conditions, low attachment supports, magnetic levitation, scaffold-based technologies. The choice most appropriate method depends on (i) origin tissue, (ii) presence absence a disease, (iii) intended application. This review summarizes approaches cancer spheroids organoids, their advantages limitations. We also highlight some challenges unresolved issues field discuss possible therapeutic applications.
Language: Английский
Citations
66
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: July 19, 2023
Abstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal spatial control cell-cell interactions. Bioprinting, while potentially capable achieving such control, is poorly suited to organoids with conserved cytoarchitectures are susceptible plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting an iron-oxide nanoparticle laden hydrogel magnetized 3D printer enable the controlled lifting, transport, deposition organoids. We identify cellulose nanofibers as both ideal biomaterial for encasing magnetic nanoparticles shear-thinning, self-healing support maintaining positioning facilitate generation assembloids. leverage SPOT create precisely arranged assembloids composed human pluripotent stem cell-derived neural patient-derived glioma In doing so, demonstrate potential platform construct which key developmental processes disease etiologies.
Language: Английский
Citations
66
Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13
Published: April 28, 2023
Heterogeneity describes the differences among cancer cells within and between tumors. It refers to describing variations in morphology, transcriptional profiles, metabolism, metastatic potential. More recently, field has included characterization of tumor immune microenvironment depiction dynamics underlying cellular interactions promoting ecosystem evolution. been found most tumors representing one challenging behaviors ecosystems. As critical factors impairing long-term efficacy solid therapy, heterogeneity leads resistance, more aggressive metastasizing, recurrence. We review role main models emerging single-cell spatial genomic technologies our understanding heterogeneity, its contribution lethal outcomes, physiological challenges consider designing therapies. highlight how dynamically evolve because leverage this unleash recognition through immunotherapy. A multidisciplinary approach grounded novel bioinformatic computational tools will allow reaching integrated, multilayered knowledge required implement personalized, efficient therapies urgently for patients.
Language: Английский
Citations
58
Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 386 - 401
Published: June 16, 2023
Organoids are in vitro model systems that mimic the complexity of organs with multicellular structures and functions, which provide great potential for biomedical tissue engineering. However, their current formation heavily relies on using complex animal-derived extracellular matrices (ECM), such as Matrigel. These often poorly defined chemical components exhibit limited tunability reproducibility. Recently, biochemical biophysical properties hydrogels can be precisely tuned, offering broader opportunities to support development maturation organoids. In this review, fundamental ECM vivo critical strategies design organoid culture summarized. Two typically derived from natural synthetic polymers applicability improve organoids presented. The representative applications incorporating into highlighted. Finally, some challenges future perspectives also discussed developing advanced technologies toward supporting research.
Language: Английский
Citations
55
Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(8), P. 523 - 539
Published: July 8, 2024
Language: Английский
Citations
54
Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1058 - 1058
Published: March 30, 2023
In the last decades three-dimensional (3D) in vitro cancer models have been proposed as a bridge between bidimensional (2D) cell cultures and vivo animal models, gold standards preclinical assessment of anticancer drug efficacy. 3D can be generated through multitude techniques, from both immortalized lines primary patient-derived tumor tissue. Among them, spheroids organoids represent most versatile promising they faithfully recapitulate complexity heterogeneity human cancers. Although their recent applications include screening programs personalized medicine, not yet established tools for studying efficacy supporting preclinical-to-clinical translation, which remains mainly based on experimentation. this review, we describe state-of-the-art evaluation agents, focusing potential contribution to replace, reduce refine experimentations, highlighting strength weakness, discussing possible perspectives overcome current challenges.
Language: Английский
Citations
51
Journal of Clinical Investigation, Journal Year: 2023, Volume and Issue: 133(18)
Published: Sept. 14, 2023
Antibody-drug conjugates (ADCs) have emerged as a revolutionary therapeutic class, combining the precise targeting ability of monoclonal antibodies with potent cytotoxic effects chemotherapeutics. Notably, ADCs rapidly advanced in field breast cancer treatment. This innovative approach holds promise for strengthening immune system through antibody-mediated cellular toxicity, tumor-specific immunity, and adaptive responses. However, development upfront acquired resistance poses substantial challenges maximizing effectiveness these therapeutics, necessitating deeper understanding underlying mechanisms. These mechanisms include antigen loss, derangements ADC internalization recycling, drug clearance, alterations signaling pathways payload target. To overcome resistance, ongoing research efforts are focused on urgently identifying biomarkers, integrating therapy approaches, designing novel payloads. Review provides an overview clinical ADCs, explores their unique immune-boosting function, while also highlighting complex safety that must be addressed. A continued focus how impact tumor microenvironment will help to identify new payloads can improve patient outcomes.
Language: Английский
Citations
45
Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(7), P. 588 - 608
Published: July 2, 2024
Language: Английский
Citations
32