Neuron, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Advanced Materials, Journal Year: 2023, Volume and Issue: 36(34)
Published: Sept. 22, 2023
Additive manufacturing (AM), which is based on the principle of layer-by-layer shaping and stacking discrete materials, has shown significant benefits in fabrication complicated implants for tissue engineering (TE). However, many native tissues exhibit anisotropic heterogenous constructs with diverse components functions. Consequently, replication biomimetic using conventional AM processes a single material challenging. Multimaterial 3D 4D bioprinting (with time as fourth dimension) emerged promising solution constructing multifunctional that can mimic host microenvironment better than single-material alternatives. Notably, 4D-printed multimaterial architectures provide time-dependent programmable dynamic promote cell activity regeneration response to external stimuli. This paper first presents typical design strategies TE applications. Subsequently, latest are discussed, along their advantages challenges. In particular, potential smart highlighted. Furthermore, this review provides insights into how facilitate realization next-generation
Language: Английский
Citations
57
Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Human brain organoids (hBOs) are in vitro, 3D, self-organizing tissue structures increasingly used for modeling development and disease. Although they traditionally lack vasculature, recent bioengineering developments enable their vascularization, which partly recapitulates neurodevelopmental processes such as neural tube angiogenesis, formation of neurovascular unit (NVU)-like structures, early barriergenesis. vascularized hBOs (vhBOs) already to model (defects in) development, vascularization efficiency other outcomes differ substantially between protocols overall shortcomings should be considered. For instance, vessel-like vhBOs do not contain blood-like flow nor form a functional blood-brain barrier (BBB). Extended characterization, standardization, the new techniques may broader applications drug transport studies.
Language: Английский
Citations
4
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(26)
Published: April 10, 2024
Bioelectricity plays a crucial role in organisms, being closely connected to neural activity and physiological processes. Disruptions the nervous system can lead chaotic ionic currents at injured site, causing disturbances local cellular microenvironment, impairing biological pathways, resulting loss of functions. Electromagnetic stimulation has ability generate internal currents, which be utilized counter tissue damage aid restoration movement paralyzed limbs. By incorporating implanted materials, electromagnetic targeted more accurately, thereby significantly improving effectiveness safety such interventions. Currently, there have been significant advancements development numerous promising strategies with diverse materials. This review provides comprehensive summary fundamental theories, modulating material application strategies, pre-clinical therapeutic effects associated for repair. It offers thorough analysis current techniques that employ materials enhance stimulation, as well potential future applications.
Language: Английский
Citations
13
Biofabrication, Journal Year: 2024, Volume and Issue: 16(3), P. 032006 - 032006
Published: May 2, 2024
Abstract Organoids have emerged as crucial platforms in tissue engineering and regenerative medicine but confront challenges faithfully mimicking native structures functions. Bioprinting technologies offer a significant advancement, especially when combined with organoid bioinks-engineered formulations designed to encapsulate both the architectural functional elements of specific tissues. This review provides rigorous, focused examination evolution impact bioprinting. It emphasizes role bioinks that integrate key cellular components microenvironmental cues more accurately replicate complexity. Furthermore, this anticipates transformative landscape invigorated by integration artificial intelligence bioprinting techniques. Such fusion promises refine bioink optimize parameters, thus catalyzing unprecedented advancements medicine. In summary, accentuates pivotal potential advancing therapies, deepening our understanding organ development, clarifying disease mechanisms.
Language: Английский
Citations
12
Food Hydrocolloids, Journal Year: 2024, Volume and Issue: 154, P. 110079 - 110079
Published: April 13, 2024
Language: Английский
Citations
10
Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(8), P. 691 - 709
Published: June 5, 2024
Language: Английский
Citations
8
Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 045029 - 045029
Published: Aug. 12, 2024
Abstract Multicellular spheroids such as microtissues and organoids have demonstrated great potential for tissue engineering applications in recent years these 3D cellular units enable improved cell–cell cell–matrix interactions. Current bioprinting processes that use multicellular building blocks limited control on post printing distribution of cell or moderate throughput efficiency. In this work, we presented a laser-assisted approach able to transfer larger structures. Cartilaginous formed by human periosteum derived cells (hPDCs) were successfully bioprinted possessing high viability the capacity undergo chondrogenic differentiation printing. Smaller hPDC with diameters ranging from ∼100 150 µ m through laser-induced forward method (LIFT) however constituted challenge. For reason novel alternative was developed termed laser induced propulsion mesoscopic objects (LIPMO) whereby bioprint up 300 m. Moreover, combined process computer aided image analysis demonstrating ‘target shoot’, automated selection, multiple large single sequence. By taking advantage target shoot system, multilayered constructs containing density fabricated.
Language: Английский
Citations
8
Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 12, 2024
Language: Английский
Citations
8
Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(4)
Published: April 1, 2024
Abstract Background Organoid technology is an emerging and rapidly growing field that shows promise in studying organ development screening therapeutic regimens. Although organoids have been proposed for a decade, concerns exist, including batch‐to‐batch variations, lack of the native microenvironment clinical applicability. Main body The concept has derived patient‐derived tumour (PDTOs) personalized drug new discovery, mitigating risks medication misuse. greater similarity between PDTOs primary tumours, more influential model will be. Recently, ‘tumour assembloids’ inspired by cell‐coculture attracted attention to complement current PDTO technology. High‐quality must reassemble critical components, multiple cell types, matrix, paracrine factors, angiogenesis microorganisms. This review begins with brief overview history PDTOs, followed approaches generating assembloids. Personalized practised; however, it remains unclear whether can predict immunotherapies, immune drugs (e.g. checkpoint inhibitors) cells tumour‐infiltrating lymphocyte, T receptor‐engineered chimeric antigen receptor‐T cell). as cancer avatars patients, be expanded stored form biobank. Conclusion Fundamental research trials are ongoing, intention use these models replace animals. Pre‐clinical immunotherapy using beneficial patients. Key Points not yet constructed key cellular non‐cellular components. should expandable editable. promising preclinical unless mature established. biobanks consensual standards urgently needed.
Language: Английский
Citations
8
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: June 25, 2024
Abstract Cell spheroids (esp. organoids) as 3D culture platforms are popular models for representing cell–cell and cell–extracellular matrix (ECM) interactions, bridging the gap between 2D cell cultures natural tissues. with spatially organized multiple types preferred gaining comprehensive insights into tissue pathophysiology constructing in vitro tissues disease because of complexities In recent years, an assembly strategy using (or living building blocks has been developed to construct complex spatial organization. Here, a overview advances multispheroid studies is provided. The different mechanisms techniques, i.e., automated directed assembly, noncontact remote programmed self‐assembly, introduced. processing steps, advantages, technical limitations existing methodologies summarized. Applications strategies modeling, drug screening, engineering, organogenesis reviewed. Finally, this review concludes by emphasizing persistent issues future perspectives, encouraging researchers adopt techniques generating advanced that better resemble real
Language: Английский
Citations
8