Microbiome, Journal Year: 2022, Volume and Issue: 10(1)
Published: Dec. 9, 2022
From a theoretical ecology point of view, microbiomes are far more complex than expected. Besides competition and competitive exclusion, cooperative microbe-microbe interactions have to be carefully considered. Metabolic dependencies among microbes likely explain co-existence in microbiota.
Language: Английский
Journal of Integrative Plant Biology, Journal Year: 2022, Volume and Issue: 64(2), P. 230 - 243
Published: Jan. 14, 2022
The root microbiome refers to the community of microbes living in association with a plant's roots, and includes mutualists, pathogens, commensals. Here we focus on recent advances study commensal which is major research object microbiome-related researches. With rapid development new technologies, plant-commensal interactions can be explored unprecedented breadth depth. Both soil environment host plant drive assembly. bulk seed bank potential commensals, plants use exudates immune responses build healthy microbial communities from available microbes. extends functional system by participating variety processes, including nutrient absorption, growth promotion, resistance biotic abiotic stresses. Plants their microbiomes have evolved adaptation strategies over time. However, there still huge gap our understanding regulatory mechanisms interactions. In this review, summarize assembly effects these development, look at prospects for promoting sustainable agricultural through microbiome.
Language: Английский
Citations
235
Nature Plants, Journal Year: 2021, Volume and Issue: 7(6), P. 814 - 825
Published: May 24, 2021
Plants grown in natural soil are colonized by phylogenetically structured communities of microbes known as the microbiota. Individual can activate microbe-associated molecular pattern (MAMP)-triggered immunity (MTI), which limits pathogen proliferation but curtails plant growth, a phenomenon growth-defence trade-off. Here, we report that, monoassociations, 41% (62 out 151) taxonomically diverse root bacterial commensals suppress Arabidopsis thaliana growth inhibition (RGI) triggered immune-stimulating MAMPs or damage-associated patterns. Amplicon sequencing 16S rRNA genes reveals that immune activation alters profile synthetic (SynComs) comprising RGI-non-suppressive strains, whereas presence RGI-suppressive strains attenuates this effect. Root colonization SynComs with different complexities and activities expression 174 core host genes, functions related to development nutrient transport. Furthermore, specifically downregulate subset immune-related genes. Precolonization plants SynComs, mutation one commensal-downregulated transcription factor, MYB15, renders more susceptible opportunistic Pseudomonas pathogens. Our results suggest modulate susceptibility pathogens either eliciting dampening MTI responses, respectively. This interplay buffers system against perturbation defence-associated inhibition, ultimately leading commensal-host homeostasis.
Language: Английский
Citations
151
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: June 11, 2022
Microbiomes are important for crop performance. However, a deeper knowledge of crop-associated microbial communities is needed to harness beneficial host-microbe interactions. Here, by assessing the assembly and functions maize microbiomes across soil types, climate zones, genotypes, we found that stem xylem selectively recruits highly conserved microbes dominated Gammaproteobacteria. We showed proportion bacterial taxa carrying nitrogenase gene (nifH) was larger in than other organs such as root leaf endosphere. Of 25 core identified sap, several isolated strains were confirmed be active nitrogen-fixers or assist with biological nitrogen fixation. On this basis, established synthetic (SynComs) consisting two diazotrophs helpers. GFP-tagged 15N isotopic dilution method demonstrated these SynComs do thrive contribute, through fixation, 11.8% total N accumulated stems. These sap represent an untapped resource can exploited increase productivity.
Language: Английский
Citations
129
Current Opinion in Plant Biology, Journal Year: 2022, Volume and Issue: 67, P. 102227 - 102227
Published: May 4, 2022
Language: Английский
Citations
79
Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 73, P. 102286 - 102286
Published: March 4, 2023
A promising, sustainable way to enhance plant health and productivity is by leveraging beneficial microbes. Beneficial microbes are natural soil residents with proven benefits for performance health. When applied in agriculture improve crop yield performance, these commonly referred as bioinoculants. Yet, despite their promising properties, bioinoculant efficacy can vary dramatically the field, hampering applicability. Invasion of rhizosphere microbiome a critical determinant success. complex phenomenon that shaped interactions local, resident host plant. Here, we explore all dimensions cross-cutting ecological theory molecular biology microbial invasion rhizosphere. We refer famous Chinese philosopher strategist Sun Tzu, who believed solutions problems require deep understanding themselves, review major biotic factors determining effectiveness.
Language: Английский
Citations
63
EMBO Reports, Journal Year: 2023, Volume and Issue: 24(9)
Published: July 20, 2023
Plants interact with a diversity of microorganisms that influence their growth and resilience, they can therefore be considered as ecological entities, namely "plant holobionts," rather than singular organisms. In plant holobiont, the assembly above- belowground microbiota is ruled by host, microbial, environmental factors. Upon microorganism perception, plants activate immune signaling resulting in secretion factors modulate composition. Additionally, metabolic interdependencies antagonism between microbes are driving forces for community assemblies. We argue complex plant-microbe intermicrobial interactions have been selected during evolution may promote survival fitness associated holobionts. As part this process, evolved metabolite-mediated strategies to selectively recruit beneficial microbiota. Some these members show host-adaptation, from which mutualism rapidly arise. also co-evolved antagonistic activities restrict proliferation high pathogenic potential prevent disease development. Co-evolution within holobionts thus ultimately drives performance.
Language: Английский
Citations
61
Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(15)
Published: April 5, 2023
Soil-dwelling microbes are the principal inoculum for root microbiota, but our understanding of microbe-microbe interactions in microbiota establishment remains fragmentary. We tested 39,204 binary interbacterial inhibitory activities vitro, allowing us to identify taxonomic signatures bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified antimicrobial 2,4-diacetylphloroglucinol (DAPG) iron chelator pyoverdine as exometabolites whose combined functions explain most activity strongly antagonistic Pseudomonas brassicacearum R401. Microbiota reconstitution with a core Arabidopsis thaliana commensals presence wild-type or mutant strains revealed niche-specific cofunction these competence determinants drivers predictable changes root-associated community. In natural environments, both corresponding biosynthetic operons enriched roots, pattern likely linked their role sinks, indicating that cofunctioning adaptive traits contributing pseudomonad pervasiveness throughout microbiota.
Language: Английский
Citations
50
FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 48(1)
Published: Dec. 12, 2023
Rhizosphere microbes play critical roles for plant's growth and health. Among them, the beneficial rhizobacteria have potential to be developed as biofertilizer or bioinoculants sustaining agricultural development. The efficient rhizosphere colonization of these is a prerequisite exerting their plant functions, but colonizing process underlying mechanisms not been thoroughly reviewed, especially nonsymbiotic rhizobacteria. This review systematically analyzed root compared it with that symbiotic pathogenic bacteria. also highlighted approaches improve efficiency proposed study rhizobacterial from holistic perspective microbiome under more natural conditions.
Language: Английский
Citations
49
Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
4
The Plant Journal, Journal Year: 2021, Volume and Issue: 109(3), P. 508 - 522
Published: Nov. 7, 2021
Soil is a living ecosystem, the health of which depends on fine interactions among its abiotic and biotic components. These form delicate equilibrium maintained through multilayer network that absorbs certain perturbations guarantees soil functioning. Deciphering principles governing within soils critical importance for their management conservation. Here, we focus microbiota discuss complexity impact composition function interaction with plants. We how physical aspects influence microbiota-plant support plant growth responses to nutrient deficiencies. predict understanding determining configuration functioning will contribute design microbiota-based strategies preserve natural resources develop more environmentally friendly agricultural practices.
Language: Английский
Citations
65