A comparative study revealed hyperspectral imaging as a potential standardized tool for the analysis of cuticle tanning over insect development DOI Creative Commons
Virginie Lacotte, Elisa Dell’Aglio, Sergio Peignier

et al.

Heliyon, Journal Year: 2023, Volume and Issue: 9(3), P. e13962 - e13962

Published: Feb. 23, 2023

Cereal-feeding beetles are a major risk for cereal crop maintenance. Cereal weevils such as Sitophilus oryzae have symbiotic intracellular bacteria that provide essential aromatic amino acid to the host biosynthesis of their cuticle building blocks. Their is an important protective barrier against biotic and abiotic stresses, providing high resistance from insecticides. Quantitative optical methods specialized in insect analysis exist, but scope use repeatability results remain limited. Here, we investigated potential Hyperspectral Imaging (HSI) standardized tool. Based on HSI, acquired time series average reflectance profiles 400 1000 nm (with bacteria) aposymbiotic (without S. exposed different nutritional stresses. We assessed phenotypic changes under diets throughout development demonstrated agreement between HSI method classically used Red-Green-Blue analysis. Then, compared both technologies laboratory conditions highlighted assets develop simple, automated, This first study showing reliability feasibility changes.

Language: Английский

Toward an Integrated Understanding of the Lepidoptera Microbiome DOI Creative Commons
Yongqi Shao, Charles J. Mason, Gary W. Felton

et al.

Annual Review of Entomology, Journal Year: 2023, Volume and Issue: 69(1), P. 117 - 137

Published: Aug. 16, 2023

Research over the past 30 years has led to a widespread acceptance that insects establish and diverse associations with microorganisms. More recently, microbiome research been accelerating in lepidopteran systems, leading greater understanding of both endosymbiont gut microorganisms how they contribute integral aspects host. Lepidoptera are associated robust assemblage microorganisms, some which may be stable routinely detected larval adult hosts, while others ephemeral transient. Certain populate can significantly hosts’ performance fitness, inconsequential. We emphasize context-dependent nature interactions between players. While our review discusses contemporary literature, there major avenues yet explored determine fundamental host–microbe potential applications for microbiome; we describe these after synthesis.

Language: Английский

Citations

38
Bacterial c-di-GMP has a key role in establishing host–microbe symbiosis DOI Creative Commons
Nancy Obeng, Anna Czerwinski,

Daniel Schütz

et al.

Nature Microbiology, Journal Year: 2023, Volume and Issue: 8(10), P. 1809 - 1819

Published: Aug. 31, 2023

Most microbes evolve faster than their hosts and should therefore drive evolution of host-microbe interactions. However, relatively little is known about the characteristics that define adaptive path to host association. Here we identified microbial traits mediate adaptation by experimentally evolving free-living bacterium Pseudomonas lurida with nematode Caenorhabditis elegans as its host. After ten passages, repeatedly observed beneficial host-specialist bacteria, improved persistence in being associated increased biofilm formation. Whole-genome sequencing revealed mutations uniformly upregulate bacterial second messenger, cyclic diguanylate (c-di-GMP). We subsequently generated mutants upregulated c-di-GMP different strains species, which consistently Comparison pseudomonad genomes from various environments underlies a variety hosts, plants humans. This study indicates fundamental for establishing

Language: Английский

Citations

32
With a little help from my friends: the roles of microbial symbionts in insect populations and communities DOI Creative Commons
Piotr Łukasik, Michał Kolasa

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1904)

Published: May 5, 2024

To understand insect abundance, distribution and dynamics, we need to the relevant drivers of their populations communities. While microbial symbionts are known strongly affect many aspects biology, lack data on effects or community processes, insects' evolutionary responses at different timescales. How these change as anthropogenic ecosystems intensify is an area intense research. Recent developments in sequencing bioinformatics permit cost-effective diversity surveys, tracking symbiont transmission, identification functions across multi-species In this review, explore how functional categories can influence life-history traits, could interactions with other species, they may processes patterns level entire We argue that insect-associated microbes should be considered important response adaptation environmental challenges opportunities. also outline emerging approaches for surveying characterizing microbiota population scales. This article part theme issue ‘Towards a toolkit global biodiversity monitoring’.

Language: Английский

Citations

11
Fitness trade-offs and the origins of endosymbiosis DOI Creative Commons
Michael A. Brockhurst, Duncan D. Cameron, Andrew P. Beckerman

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(4), P. e3002580 - e3002580

Published: April 12, 2024

Endosymbiosis drives evolutionary innovation and underpins the function of diverse ecosystems. The mechanistic origins symbioses, however, remain unclear, in part because early events are obscured by subsequent evolution genetic drift. This Essay highlights how experimental studies facultative, host-switched, synthetic symbioses revealing important role fitness trade-offs between within-host free-living niches during early-stage new symbiotic associations. mutational targets underpinning such commonly regulatory genes, that single mutations have major phenotypic effects on multiple traits, thus enabling reinforcing transition to a lifestyle.

Language: Английский

Citations

9
Plant pathogenesis: Toward multidimensional understanding of the microbiome DOI Creative Commons
Tianxing Lv, Chengfang Zhan, Qianqian Pan

et al.

iMeta, Journal Year: 2023, Volume and Issue: 2(3)

Published: July 16, 2023

Abstract Single pathogen‐targeted disease management measure has shown drawbacks in field efficacy under the scenario of global change. An in‐depth understanding plant pathogenesis will provide a promising solution but faces challenges emerging paradigm involving microbiome. While beneficial impact microbiome is well characterized, their potential role facilitating pathological processes so far remained largely overlooked. To address these unsolved controversies and challenges, we hereby highlight pathobiome, disease‐assisting portion hidden microbiome, paradigm. We review detrimental actions mediated by pathobiome at multiple scales further discuss how natural human triggers result prevalence which would probably clue to mitigation epidemics. Collectively, article advance current insight into also pave new way cope with upward trends designing pathobiome‐targeted measure.

Language: Английский

Citations

17
Comparative Genomics of Pesticide-Degrading Enterococcus Symbionts of Spodoptera frugiperda (Lepidoptera: Noctuidae) Leads to the Identification of Two New Species and the Reappraisal of Insect-Associated Enterococcus Species DOI
Ana Flávia Freitas Gomes, Luís Gustavo de Almeida, Fernando L. Cônsoli

et al.

Microbial Ecology, Journal Year: 2023, Volume and Issue: 86(4), P. 2583 - 2605

Published: July 11, 2023

Language: Английский

Citations

16
Variations on a theme: factors regulating interaction between Diaphorina citri and “Candidatus Liberibacter asiaticus” vector and pathogen of citrus huanglongbing DOI Creative Commons
Saeed Hosseinzadeh, Michelle Heck

Current Opinion in Insect Science, Journal Year: 2023, Volume and Issue: 56, P. 101025 - 101025

Published: March 27, 2023

Diaphorina citri, the Asian citrus psyllid, is a vector of Candidatus Liberibacter asiaticus (CLas), causal agent huanglongbing (HLB), world's most serious disease citrus. Owing to relevancy and urgency HLB research, study transmission biology in pathosystem has been significant area research. The focus this article summarize synthesize recent advancements between D. citri CLas create an updated view research landscape identify avenues for future Variability appears play important role by citri. We advocate that it understand genetic basis environmental factors contributing how variation may be exploited develop improve control tactics.

Language: Английский

Citations

14
Refocusing the microbial rare biosphere concept through a functional lens DOI
Elena Litchman, Sébastien Villéger, Lucie Zinger

et al.

Trends in Ecology & Evolution, Journal Year: 2024, Volume and Issue: 39(10), P. 923 - 936

Published: July 9, 2024

Language: Английский

Citations

5
Inducing novel endosymbioses by implanting bacteria in fungi DOI Creative Commons
Gabriel H. Giger,

Chantal Ernst,

Ingrid Richter

et al.

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Language: Английский

Citations

5
Rational engineering of a synthetic insect-bacterial mutualism DOI Creative Commons
Yinghua Su,

Ho-Chen Lin,

Li Szhen Teh

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(18), P. 3925 - 3938.e6

Published: Aug. 12, 2022

Language: Английский

Citations

20