The Discovery of Extracellular Vesicles and Their Emergence as a Next-Generation Therapy DOI
Alin Rai, Bethany Claridge, Jonathan Lozano

et al.

Circulation Research, Journal Year: 2024, Volume and Issue: 135(1), P. 198 - 221

Published: June 20, 2024

From their humble discovery as cellular debris to cementing natural capacity transfer functional molecules between cells, the long-winded journey of extracellular vesicles (EVs) now stands at precipice a next-generation cell-free therapeutic tool revolutionize modern-day medicine. This perspective provides snapshot EVs emergence vibrant field biology and renaissance they usher in biomedical sciences agents for cardiovascular pathologies. Rapid development bioengineered is providing innovative opportunities overcome biological challenges such potency, cargo loading enhanced secretion, targeting circulation half-life, localized sustained delivery strategies, approaches enhance systemic circulation, uptake lysosomal escape, logistical hurdles encompassing scalability, cost, time. A multidisciplinary collaboration beyond extends chemistry, physics, biomaterials, nanotechnology, allowing rapid designer that are entering late-stage human clinical trials.

Language: Английский

Bacteria in cancer initiation, promotion and progression DOI
Geniver El Tekle, Wendy S. Garrett

Nature reviews. Cancer, Journal Year: 2023, Volume and Issue: 23(9), P. 600 - 618

Published: July 3, 2023

Language: Английский

Citations

155
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy DOI Open Access

Yi You,

Yu Tian, Zhaogang Yang

et al.

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 7(7), P. 887 - 900

Published: Jan. 12, 2023

Language: Английский

Citations

126
Comprehensive isolation of extracellular vesicles and nanoparticles DOI Open Access
Qin Zhang, Dennis K. Jeppesen,

James N. Higginbotham

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(5), P. 1462 - 1487

Published: March 13, 2023

Language: Английский

Citations

123
Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading DOI Creative Commons
Julia Rädler, Dhanu Gupta, Antje M. Zickler

et al.

Molecular Therapy, Journal Year: 2023, Volume and Issue: 31(5), P. 1231 - 1250

Published: Feb. 20, 2023

Extracellular vesicles (EVs) are gaining increasing attention for diagnostic and therapeutic applications in various diseases. These natural nanoparticles benefit from favorable safety profiles unique biodistribution capabilities, rendering them attractive drug-delivery modalities over synthetic analogs. However, the widespread use of EVs is limited by technological shortcomings biological knowledge gaps that fail to unravel their heterogeneity. An in-depth understanding biogenesis crucial unlocking full potential. Here, we explore how about EV can be exploited bioengineering load protein or nucleic acid cargos into onto EVs. We summarize more than 75 articles discuss findings on formation composition exosomes microvesicles, revealing multiple pathways may stimulation and/or cargo dependent. Our analysis further identifies key regulators loading this integrated develop engineered biotherapeutics.

Language: Английский

Citations

117
Extracellular vesicles: a rising star for therapeutics and drug delivery DOI Creative Commons

Shuang Du,

Yucheng Guan,

Aihua Xie

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: July 20, 2023

Abstract Extracellular vesicles (EVs) are nano-sized, natural, cell-derived that contain the same nucleic acids, proteins, and lipids as their source cells. Thus, they can serve natural carriers for therapeutic agents drugs, have many advantages over conventional nanocarriers, including low immunogenicity, good biocompatibility, blood – brain barrier penetration, capacity gene delivery. This review first introduces classification of EVs then discusses several currently popular methods isolating purifying EVs, EVs-mediated drug delivery, functionalization carriers. Thereby, it provides new avenues development EVs-based strategies in different fields medicine. Finally, highlights some challenges future perspectives with regard to clinical application EVs. Graphical

Language: Английский

Citations

98
Current research trends of nanomedicines DOI Creative Commons
Qiuyue Liu, Jiahui Zou, Zhongjian Chen

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 13(11), P. 4391 - 4416

Published: May 20, 2023

Owing to the inherent shortcomings of traditional therapeutic drugs in terms inadequate efficacy and toxicity clinical treatment, nanomedicine designs have received widespread attention with significantly improved reduced non-target side effects. Nanomedicines hold tremendous theranostic potential for treating, monitoring, diagnosing, controlling various diseases are attracting an unfathomable amount input research resources. Against backdrop exponentially growing number publications, it is imperative help audience get a panorama image activities field nanomedicines. Herein, this review elaborates on development trends nanomedicines, emerging nanocarriers, vivo fate safety their extensive applications. Moreover, challenges obstacles hindering translation nanomedicines also discussed. The elaboration aspects may enlighten readers set route future endeavors.

Language: Английский

Citations

80
Exosomes from young healthy human plasma promote functional recovery from intracerebral hemorrhage via counteracting ferroptotic injury DOI Creative Commons
Wenqin Yang, Ning Ding,

Ran Luo

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 27, P. 1 - 14

Published: March 23, 2023

Intracerebral hemorrhage (ICH), as a type of life-threatening and highly disabled disease, has limited therapeutic approaches. Here, we show that exosomes derived from young healthy human plasma exhibiting typical features could facilitate functional recovery ICH mice. When these are intraventricularly delivered into the brain after ICH, they mainly distribute around hematoma be internalized by neuronal cells. Strikingly, administration markedly enhanced behavioral mice through reducing injury cell ferroptosis. MiRNA sequencing revealed microRNA-25-3p (miR-25-3p) was differentially expressed miRNA in plasma, compared with old control. Importantly, miR-25-3p mimicked treatment effect on improvement, mediated neuroprotective against ferroptosis ICH. Furthermore, luciferase assay western blotting data illustrated P53 assumed role downstream effector miR-25-3p, thereby regulating SLC7A11/GPX4 pathway to counteract Taken together, findings firstly reveal improve counteracting ferroptotic P53/SLC7A11/GPX4 axis Given easy availability exosomes, our study provides potent strategy for patients quick clinical translation near future.

Language: Английский

Citations

78
Apoptotic bodies: bioactive treasure left behind by the dying cells with robust diagnostic and therapeutic application potentials DOI Creative Commons
Lina Yu,

Guanxiong Zhu,

Zeyu Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: July 12, 2023

Abstract Apoptosis, a form of programmed cell death, is essential for growth and tissue homeostasis. Apoptotic bodies (ApoBDs) are extracellular vesicles (EVs) released by dying cells in the last stage apoptosis were previously regarded as debris dead cells. Recent studies unraveled that ApoBDs not but bioactive treasure left behind with an important role intercellular communications related to human health various diseases. Defective clearance infected-cells-derived possible etiology some Therefore, it necessary explore function mechanism action different physiological pathological conditions. advances have elucidated immunomodulatory, virus removal, vascular protection, regenerative, disease diagnostic potential ApoBDs. Moreover, can be used drug carriers enhancing stability, cellular uptake, targeted therapy efficacy. These reports from literature indicate hold promising diagnosis, prognosis, treatment diseases, including cancer, systemic inflammatory cardiovascular regeneration. This review summarizes recent ApoBDs-related research discusses diseases well challenges prospects ApoBDs-based therapeutic applications. Graphical

Language: Английский

Citations

63
A DNA-based hydrogel for exosome separation and biomedical applications DOI Creative Commons
Jianpu Tang, Xuemei Jia, Qian Li

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(28)

Published: July 3, 2023

Exosomes (EXOs) have been proven as biomarkers for disease diagnosis and agents therapeutics. Great challenge remains in the separation of EXOs with high-purity low-damage from complex biological media, which is critical downstream applications. Herein, we report a DNA-based hydrogel to realize specific nondestructive media. The separated were directly utilized detection human breast cancer clinical samples, well applied therapeutics myocardial infarction rat models. materials chemistry basis this strategy involved synthesis ultralong DNA chains via an enzymatic amplification, formation hydrogels through complementary base-pairing. These that contained polyvalent aptamers able recognize bind receptors on EXOs, efficient binding ensured selective media into further formed networked hydrogel. Based hydrogel, rationally designed optical modules introduced exosomal pathogenic microRNA, achieved classification patients versus healthy donors 100% precision. Furthermore, mesenchymal stem cell–derived was proved significant therapeutic efficacy repairing infarcted myocardium We envision hydrogel–based bioseparation system promising powerful biotechnology, will promote development extracellular vesicles nanobiomedicine.

Language: Английский

Citations

59
Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury DOI Creative Commons

Ao Fang,

Yifan Wang,

Naiyu Guan

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 7, 2023

Abstract The transplantation of mesenchymal stem cells-derived secretome, particularly extracellular vesicles is a promising therapy to suppress spinal cord injury-triggered neuroinflammation. However, efficient delivery the injured cord, with minimal damage, remains challenge. Here we present device for treat injury. We show that incorporating cells and porous microneedles enables vesicles. demonstrate topical application lesion beneath dura, does not damage lesion. evaluate efficacy our in contusive injury model find it reduces cavity scar tissue formation, promotes angiogenesis, improves survival nearby tissues axons. Importantly, sustained at least 7 days results significant functional recovery. Thus, provides an platform treatment.

Language: Английский

Citations

58