Nutrients,
Journal Year:
2025,
Volume and Issue:
17(5), P. 805 - 805
Published: Feb. 26, 2025
Background/Objectives:
There
is
limited
information
on
human
milk
(HM)
microbiome
composition
and
function
in
Latin
America.
Also,
interactions
between
HM
constituents
its
have
received
partial
attention.
Objective:
To
characterize
the
microbiota
considering
lactation
stages
(colostrum,
transition,
mature
HM)
free
glutamate
concentrations
Ecuadorian
mothers.
Methods:
We
recruited
20
mothers
that
gave
birth
to
normal
full-term
babies
donated
colostrum,
milk.
Samples
were
assessed
by
16S
rRNA
gene
sequencing
Oxford
Nanopore
Technologies
(ONT).
Free
measured
proton
nuclear
magnetic
resonance
(NMR)
spectroscopy.
Results:
For
each
stage
order
of
frequency,
majority
ASVs
assigned
Staphylococcus,
Streptococcus,
(Firmicutes);
Escherichia,
Acinetobacter,
(Proteobacteria);
Corynebacterium,
Lactobacillus,
Cutibacterium,
(Actinobacteriota);
Chryseobacterium,
Flavobacterium
(Bacteroidota).
Alfa
diversity
was
similar
samples
tended
be
higher
intended
for
male
infants.
observed
significant
differences
qualitative
β-diversity
metrics
with
low
high
concentrations.
Functional
predictions
demonstrated
presence
polyamine
biosynthesis
II
super
pathway
Conclusions:
The
core
bacterial
components
women
those
reported
from
different
parts
world,
variations
at
genus
level.
dynamics
need
studied
maternal
production
metabolism
better
understand
optimal
infant
nutrition.
Class
1
cytokine
receptors
transmit
signals
through
the
membrane
by
a
single
transmembrane
helix
to
an
intrinsically
disordered
cytoplasmic
domain
that
lacks
kinase
activity.
While
specific
binding
phosphoinositides
has
been
reported
for
prolactin
receptor
(PRLR),
role
of
lipids
in
PRLR
signaling
is
unclear.
Using
integrative
approach
combining
nuclear
magnetic
resonance
spectroscopy,
cellular
experiments,
computational
modeling,
and
simulation,
we
demonstrate
co-structure
formation
intracellular
human
PRLR,
constituent
phosphoinositide-4,5-bisphosphate
(PI(4,5)P
2
)
FERM-SH2
Janus
(JAK2).
We
find
complex
leads
accumulation
PI(4,5)P
at
interface
mutation
residues
identified
interact
specifically
with
negatively
affects
PRLR-mediated
activation
signal
transducer
activator
transcription
5
(STAT5).
Facilitated
formation,
membrane-proximal
region
arranges
into
extended
structure.
suggest
formed
between
JAK2,
locks
juxtamembrane
structure,
enabling
relay
from
extracellular
upon
ligand
binding.
exists
different
states
which
speculate
could
be
relevant
turning
on
off.
Similar
co-structures
may
other
non-receptor
tyrosine
kinases
their
receptors.
Frontiers in Endocrinology,
Journal Year:
2024,
Volume and Issue:
15
Published: Jan. 22, 2024
Introduction
Hormones
play
a
vital
role
in
development
from
conception
to
birth
and
throughout
the
human
lifespan.
These
periods
are
logically
divided
into
fetal
development,
pre-pubertal
growth,
puberty,
adulthood.
Deviations
standard
physiological
levels
release
patterns
of
constituent
hormones
can
lead
pathology
affecting
normal
developmental
trajectory.
Research
is
ongoing
better
understand
mechanisms
these
how
their
modulation
affects
development.
Methods
This
article
focuses
on
recent
developments
understanding
We
also
cover
discoveries
signaling
pathways
hormonal
regulation.
Results
New
continuing
research
functional
hormone
regulation
sex
hormones,
gonadotropic
growth
insulin-like
factor,
thyroid
hormone,
interconnectedness
each
axes.
Currently,
abundance
work
fertility
correction
based
an
individual’s
condition
stage
life.
Discussion
Continuing
needed
fully
long-term
effects
sexual
The
parallel
endocrine
axes
should
be
more
thoroughly
investigated
help
improve
safety
efficacy
pharmacotherapeutics.
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 8, 2024
Cellular
agriculture
is
an
innovative
technology
for
manufacturing
sustainable
agricultural
products
as
alternative
to
traditional
agriculture.
While
most
cellular
predominantly
centered
on
the
production
of
cultured
meat,
there
a
growing
demand
understanding
techniques
involved
in
dairy
within
This
review
focuses
current
status
sector
and
technical
challenges
cell-cultured
milk
production.
has
been
classified
into
fermentation-based
animal
cell
culture-based
Currently,
various
companies
synthesize
components
through
precision
fermentation
technology.
Nevertheless,
several
startup
are
pursuing
cell-based
technology,
driven
by
public
concerns
regarding
genetically
modified
organisms
Hence,
this
offers
up-to-date
exploration
produce
components,
specifically
emphasizing
structural,
functional,
productive
aspects
mammary
epithelial
cells,
providing
new
information
industry
academia.
Journal of Dairy Science,
Journal Year:
2024,
Volume and Issue:
107(9), P. 6696 - 6716
Published: April 11, 2024
This
study
is
aimed
at
investigating
the
effects
of
dietary
supplementation
with
Artemisia
ordosica
crude
polysaccharides
(AOCP)
on
lactation
performance,
antioxidant
status,
and
immune
status
lactating
donkeys
analyzing
rectal
microbiomes
serum
metabolomes.
Fourteen
Dezhou
similar
age
(6.16
±
0.67
yr
BW
SD),
weight
(250.06
25.18
kg),
DIM
(39.11
7.42
d),
average
parity
3
were
randomly
allocated
into
2
treatments:
a
control
group
(CON,
basal
diet)
an
AOCP
(AOCP,
diet
1.0
g/kg
DM
AOCP).
Ten
weeks
allotted
for
experiment,
wk
adaptation,
8
collecting
data
samples.
The
results
showed
that
donkey
diets
increased
including
DMI,
milking
yield,
estimated
milk
solids-corrected
milk,
ECM,
fat
protein
lactose
TS
SNF
yield.
digestibility
DM,
CP,
ADF,
NDF
was
in
compared
CON
group.
concentrations
IgA,
IgG,
IgM,
activities
superoxide
dismutase,
catalase,
total
capacity
serum.
decreased
tumor
necrosis
factor-α,
nitric
oxide,
reactive
oxygen
species,
malondialdehyde
Compared
group,
propionate,
butyrate,
isovalerate,
VFA
feces
(P
<
0.05).
addition
to
diversity
(Shannon
index)
altered
structure
microflora.
As
result
supplementation,
there
has
been
significant
improvement
colonization
beneficial
bacteria,
Lactobacillus,
Unclassified_f_Prevotellacea,
Ruminococcus,
Fibrobacter
genera.
In
contrast,
decrease
Clostridium_sensu_stricto_1
bacterial
genus
other
pathogenic
bacteria
observed.
Meanwhile,
metabolomics
analysis
found
upregulated
metabolites
l-tyrosine
content
while
downregulating
9(S)-HODE,
choline,
sucrose,
lysophosphatidylcholine
(LysoPC)
(18:0),
LysoPC
(18:1(9Z)),
(20:2(11Z,14Z))
concentrations.
These
involved
PPAR
signaling
pathway,
prolactin
glycerophospholipid
metabolism,
carbohydrate
digestion
absorption,
tyrosine
metabolism
pathways,
which
mainly
related
capacity,
responses,
donkeys.
consequence
feeding
diets,
abundant,
metabolism-related
pathways
enriched,
may
enhance
performance
Therefore,
supplementing
desirable
strategy
improve
health
performance.
Diagnostics,
Journal Year:
2025,
Volume and Issue:
15(2), P. 191 - 191
Published: Jan. 15, 2025
Background/Objectives:
The
causes
of
low
milk
supply
are
multifactorial,
including
factors
such
as
gene
mutations,
endocrine
disorders,
and
infrequent
removal.
These
affect
the
functional
capacity
mammary
gland
and,
potentially,
concentrations
components.
This
study
aimed
to
investigate
differences
in
composition
between
mothers
with
normal
develop
predictive
machine
learning
models
for
identifying
supply.
Methods:
Twenty-four-hour
production
measurements
were
conducted
using
test-weigh
method.
An
array
components
was
measured
58
women
(<600
mL/24
h)
106
(≥600
h).
Machine
algorithms
employed
prediction
integrating
maternal
infant
characteristics.
Results:
Among
six
tested,
deep
gradient
boosting
machines
methods
had
best
performance
metrics.
best-performing
model,
incorporating
14
characteristics,
achieved
an
accuracy
87.9%,
area
under
precision-recall
curve
(AUPRC)
0.893,
receiver
operating
characteristic
(AUC)
0.917.
Additionally,
a
simplified
optimised
clinical
applicability,
maintained
reasonable
78.8%,
AUPRC
0.776,
AUC
0.794.
Conclusions:
findings
demonstrate
potential
predict
high
accuracy.
Integrating
characteristics
offers
practical
approach
identify
at
risk
supply,
facilitating
timely
interventions
support
breastfeeding
ensure
adequate
nutrition.
