Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

European Journal of Immunology, Journal Year: 2021, Volume and Issue: 51(12), P. 2708 - 3145

Published: Dec. 1, 2021

Abstract The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes functional assays all major human murine immune cell subsets. Notably, contain helpful tables highlighting differences between cells. Another useful feature this is analysis clinical samples with examples applications in context autoimmune diseases, cancers as well acute chronic infectious diseases. Furthermore, there are detailing tips, tricks pitfalls avoid. All written peer‐reviewed by leading experts immunologists, making an essential state‐of‐the‐art handbook for basic researchers.

Language: Английский

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Metabolic adaptation of lymphocytes in immunity and disease

Immunity, Journal Year: 2022, Volume and Issue: 55(1), P. 14 - 30

Published: Jan. 1, 2022

Adaptive immune responses mediated by T cells and B are crucial for protective immunity against pathogens tumors. Differentiation function of require dynamic reprogramming cellular metabolism. Metabolic inputs, pathways, enzymes display remarkable flexibility heterogeneity, especially in vivo. How metabolic plasticity adaptation dictate functional specialization is fundamental to our understanding therapeutic modulation the system. Extensive progress has been made characterizing effects networks on cell fate discrete microenvironments or immunological contexts. In this review, we summarize how rewiring metabolism determines outcome adaptive vivo, with a focus metabolites, nutrients, driver genes immunometabolism instruct programming during infection, inflammation, cancer mice humans. Understanding context-dependent remodeling will manifest legitimate opportunities intervention human disease.

Language: Английский

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Warburg effect in colorectal cancer: the emerging roles in tumor microenvironment and therapeutic implications

Journal of Hematology & Oncology, Journal Year: 2022, Volume and Issue: 15(1)

Published: Nov. 1, 2022

Colorectal cancer (CRC) is the third most common and second leading cause of cancer-related death worldwide. Countless CRC patients undergo disease progression. As a hallmark cancer, Warburg effect promotes metastasis remodels tumor microenvironment, including promoting angiogenesis, immune suppression, cancer-associated fibroblasts formation drug resistance. Targeting metabolism would be promising method for treatment CRC. In this review, we summarize information about roles in microenvironment to elucidate mechanisms governing identify novel targets therapy.

Language: Английский

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Immunogenic Cell Death Augmented by Manganese Zinc Sulfide Nanoparticles for Metastatic Melanoma Immunotherapy

ACS Nano, Journal Year: 2022, Volume and Issue: 16(9), P. 15471 - 15483

Published: Aug. 18, 2022

Both T-cell deprivation and insufficient tumor immunogenicity seriously hinder the efficacy of immune-mediated destruction in melanoma. In this work, an amphiphilic polyethylene glycol-poly(2-hexoxy-2-oxo-1,3,2-dioxaphospholane) copolymer with a thermally sensitive flowable core (mPEG-b-PHEP) was chosen to incorporate IR780 dye manganese zinc sulfide nanoparticles (ZMS) form polymer micelles (denoted PPIR780-ZMS), which precisely controlled release ZMS after being triggered by near-infrared light (NIR). Mn2+-mediated chemodynamic therapy (CDT) photothermal trigger boosted generation reactive oxygen species (ROS), making PPIR780-ZMS smart bomblets vivo. It demonstrated that could maximize immunogenic cell death (ICD) cancer, is characterized abundant damage-associated molecular pattern (DAMP) exposure. As result, cytotoxic T cells (CD8+) helper (CD4+) expanded infiltrated neoplastic foci, further reprogrammed suppressive microenvironment (TME) against primary pulmonary metastases safe systemic cytokine expression. addition, cGAS-STING signaling pathway activation enhanced antitumor immunity nanocomposite, providing practical strategy for expanding use Mn-based nanostructures.

Language: Английский

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‘Stem-like’ precursors are the fount to sustain persistent CD8+ T cell responses

Nature Immunology, Journal Year: 2022, Volume and Issue: 23(6), P. 836 - 847

Published: May 27, 2022

Language: Английский

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T Cell Metabolism in Infection

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 14, 2022

T lymphocytes (T cells) are divided into two functionally different subgroups the CD4+ helper cells (Th) and CD8+ cytotoxic (CTL). Adequate CD4 CD8 cell activation to proliferation, clonal expansion effector function is crucial for efficient clearance of infection by pathogens. Failure do so may lead exhaustion. Upon antigen presenting cells, undergo metabolic reprograming that support functions. In this review we will discuss how dictates functionality during viral infections using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) human immunodeficiency virus (HIV) as examples. Moreover, briefly programs bacterial exemplified Mycobacterium tuberculosis (MT) infection.

Language: Английский

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Simultaneous Photoactivation of cGAS‐STING Pathway and Pyroptosis by Platinum(II) Triphenylamine Complexes for Cancer Immunotherapy

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(43)

Published: Aug. 18, 2022

Abstract Activation of the cyclic GMP‐AMP synthase‐stimulator interferon gene (cGAS‐STING) pathway is a potent anticancer immunotherapeutic strategy, and induction pyroptosis feasible way to stimulate immune responses. Herein, two Pt II complexes ( Pt1 Pt2 ) were designed as photoactivators cGAS‐STING pathway. In response light irradiation, could damage mitochondrial/nuclear DNA nuclear envelope activate pathway, concurrently induce in cancer cells, which evoked an intense vitro vivo. Overall, we present first photoactivator may provide innovative design strategy for immunotherapy.

Language: Английский

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Cyclooxygenase-2-Prostaglandin E2 pathway: A key player in tumor-associated immune cells

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 27, 2023

Cyclooxygenases-2 (COX-2) and Prostaglandin E2 (PGE2), which are important in chronic inflammatory diseases, can increase tumor incidence promote growth metastasis. PGE2 binds to various prostaglandin E receptors activate specific downstream signaling pathways such as PKA pathway, β-catenin NF-κB pathway PI3K/AKT all of play roles biological pathological behavior. Nonsteroidal anti-inflammatory drugs (NSAIDs), COX-2 inhibitors, EP antagonists anti-tumor immune evasion. The COX-2-PGE2 promotes evasion by regulating myeloid-derived suppressor cells, lymphocytes (CD8+ T CD4+ cells natural killer cells), antigen presenting (macrophages dendritic cells). Based on conventional treatment, the addition inhibitors or may enhance immunotherapy response escape. However, there still a lot challenges cancer immunotherapy. In this review, we focus how affects tumor-associated cells.

Language: Английский

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Innate metabolic responses against viral infections

Nature Metabolism, Journal Year: 2022, Volume and Issue: 4(10), P. 1245 - 1259

Published: Oct. 20, 2022

Language: Английский

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Metabolic programs tailor T cell immunity in viral infection, cancer, and aging

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(3), P. 378 - 395

Published: March 1, 2022

Language: Английский

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