Progression of herpesvirus infection remodels mitochondrial organization and metabolism DOI Creative Commons
Simon Leclerc, Alka Gupta, Visa Ruokolainen

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(4), P. e1011829 - e1011829

Published: April 15, 2024

Viruses target mitochondria to promote their replication, and infection-induced stress during the progression of infection leads regulation antiviral defenses mitochondrial metabolism which are opposed by counteracting viral factors. The precise structural functional changes that underlie how react remain largely unclear. Here we show extensive transcriptional remodeling protein-encoding host genes involved in respiratory chain, apoptosis, organization as herpes simplex virus type 1 lytic proceeds from early late stages infection. High-resolution microscopy interaction analyses unveiled emergence rough, thin, elongated relocalized perinuclear area, a significant increase number clustering endoplasmic reticulum-mitochondria contact sites, thickening shortening cristae. Finally, metabolic demonstrated reactivation ATP production is accompanied increased Ca 2+ content proton leakage proceeds. Overall, triggered invasion tightly connected

Language: Английский

Oxidative Damage and Post-COVID Syndrome: A Cross-Sectional Study in a Cohort of Italian Workers DOI Open Access
Angela Stufano, Camilla Isgrò, Luigi Leonardo Palese

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7445 - 7445

Published: April 18, 2023

In addition to the acute symptoms after infection, patients and society are also being challenged by long-term effects of COVID-19, known as long COVID. Oxidative stress, a pivotal point in pathophysiology could potentially be involved development post-COVID syndrome. The aim present study was evaluate relationship between changes oxidative status persistence long-COVID workers with previous mild COVID-19 infection. A cross-sectional conducted among 127 employees an Italian university (80 47 healthy subjects). TBARS assay used detect malondialdehyde serum levels (MDA), while total hydroperoxide (TH) production measured d-ROMs kit. significant difference mean MDA values found previously infected subjects controls (4.9 µm vs. 2.8 µm, respectively). Receiver–operating characteristic (ROC) curves showed high specificity good sensibility (78.7% 67.5%, respectively) for levels. random forest classifier identified hematocrit value, levels, IgG titer against SARS-CoV-2 features highest predictive value distinguishing 34 from 46 asymptomatic subjects. damage persists suggesting possible role stress mediators pathogenesis

Language: Английский

Citations

29
An Immunocompetent Mongolian Gerbil Model for Hepatitis E Virus Genotype 1 Infection DOI
Tianxu Liu, Qiyu He, Xinyue Yang

et al.

Gastroenterology, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Language: Английский

Citations

15
Pro-inflammatory macrophage activation does not require inhibition of oxidative phosphorylation DOI Creative Commons
Andréa B. Ball, Anthony E. Jones,

Kaitlyn B. Nguyễn

et al.

EMBO Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

Citations

1
A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients DOI Creative Commons
Yered Pita-Juárez, Dimitra Karagkouni, Nikolaos Kalavros

et al.

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 14, 2025

Abstract Background The molecular underpinnings of organ dysfunction in severe COVID-19 and its potential long-term sequelae are under intense investigation. To shed light on these the context liver function, we perform single-nucleus RNA-seq spatial transcriptomic profiling livers from 17 decedents. Results We identify hepatocytes positive for SARS-CoV-2 RNA with an expression phenotype resembling infected lung epithelial cells, a central role pro-fibrotic TGFβ signaling cell–cell communications network. Integrated analysis comparisons healthy controls reveal extensive changes cellular composition states liver, providing underpinning hepatocellular injury, ductular reaction, pathologic vascular expansion, fibrogenesis characteristic cholangiopathy. also observe Kupffer cell proliferation erythrocyte progenitors first time human single-cell atlas. Despite absence clinical acute injury phenotype, endothelial is dramatically impacted COVID-19, concomitantly alterations profibrogenic activation reactive cholangiocytes mesenchymal cells. Conclusions Our atlas provides novel insights into physiology pathology forms foundational resource investigation understanding.

Language: Английский

Citations

1
PI3K/AKT mediated De novo fatty acid synthesis regulates RIG-1/MDA-5-dependent type I IFN responses in BVDV-infected CD8+T cells DOI
Shanshan Liu,

Tongtong Bai,

Tao-lin Que

et al.

Veterinary Microbiology, Journal Year: 2024, Volume and Issue: 291, P. 110034 - 110034

Published: Feb. 28, 2024

Language: Английский

Citations

6
Thermal facial image analyses reveal quantitative hallmarks of aging and metabolic diseases DOI
Zhengqing Yu,

Yong Zhou,

Kehang Mao

et al.

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(7), P. 1482 - 1493.e7

Published: July 1, 2024

Language: Английский

Citations

6
Plasma metabolomic characterization of SARS-CoV-2 Omicron infection DOI Creative Commons
Xue Li, Yimeng Liu, Guiying Xu

et al.

Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(4)

Published: April 19, 2023

Omicron variants of SARS-CoV-2 have spread rapidly worldwide; however, most infected patients mild or no symptoms. This study aimed to understand the host response infections by performing metabolomic profiling plasma. We observed that triggered an inflammatory and innate immune, adaptive immunity was suppressed, including reduced T-cell immunoglobulin antibody production. Similar original strain circulating in 2019, developed anti-inflammatory accelerated energy metabolism infection. However, differential regulation macrophage polarization neutrophil function has been infections. Interferon-induced antiviral not as strong The increased antioxidant capacity liver detoxification more than strain. Hence, these findings suggest cause weaker alterations immune responses

Language: Английский

Citations

16
Redox Regulation and Metabolic Dependency of Zika Virus Replication: Inhibition by Nrf2-Antioxidant Response and NAD(H) Antimetabolites DOI
Bikash R. Sahoo, Alexandra A. Crook, Aryamav Pattnaik

et al.

Journal of Virology, Journal Year: 2023, Volume and Issue: 97(2)

Published: Jan. 23, 2023

Zika virus (ZIKV) is a major public health concern of international proportions. While the incidence ZIKV infections has declined substantially in recent years, potential for reemergence or reintroduction remains high.

Language: Английский

Citations

14
Non-human primate model of long-COVID identifies immune associates of hyperglycemia DOI Creative Commons
Clovis S. Palmer, Chrysostomos Perdios, Mohamed Abdel‐Mohsen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 20, 2024

Hyperglycemia, and exacerbation of pre-existing deficits in glucose metabolism, are manifestations the post-acute sequelae SARS-CoV-2. Our understanding metabolic decline after acute COVID-19 remains unclear due to lack animal models. Here, we report a non-human primate model SARS-CoV-2 using infected African green monkeys. Using this model, identify dysregulated blood chemokine signature during that correlates with elevated persistent hyperglycemia four months post-infection. Hyperglycemia also liver glycogen levels, but there is no evidence substantial long-term replication pancreas. Finally, favorable glycemic effect mRNA vaccine, administered on day 4 Together, these data suggest monkey exhibits important similarities humans can be utilized assess therapeutic candidates combat COVID-related defects.

Language: Английский

Citations

5
Unveiling the growing significance of metabolism in modulating immune cell function: exploring mechanisms and implications; a review DOI Open Access
Nicholas Aderinto, Muili Abdulbasit, Tangmi Djabo Eric Adrien

et al.

Annals of Medicine and Surgery, Journal Year: 2023, Volume and Issue: 85(11), P. 5511 - 5522

Published: Sept. 13, 2023

Immunometabolism has emerged as a rapidly growing field of research, holding significant promise for personalised medicine and precision immunotherapy. This review explores the intricate relationship between immune function metabolic processes, emphasising their profound impact on various immune-related disorders. Understanding how dysregulation contributes to pathogenesis these disorders remains critical research gap. Therefore, this aims bridge that gap by examining key pathways involved specific implications in cell function. Key pathways, including glycolysis, mitochondrial metabolism, fatty acid amino are discussed context Dysregulation can disrupt activation, differentiation, overall function, contributing disease pathogenesis. alterations' molecular mechanisms is essential developing targeted therapeutic interventions. The also emphasises importance unique profiles individuals influence treatment outcomes, highlighting need tailored approaches. Integrating profiling into clinical practice enhance efficacy improve patient outcomes. Investigating significance immunometabolism diverse contexts will facilitate translation findings practice. Moreover, refining strategies based individual contribute advancing

Language: Английский

Citations

11