Cell stem cell,
Journal Year:
2021,
Volume and Issue:
29(2), P. 209 - 216.e4
Published: Dec. 21, 2021
In
human
embryos,
the
initiation
of
transcription
(embryonic
genome
activation
[EGA])
occurs
by
eight-cell
stage,
but
its
exact
timing
and
profile
are
unclear.
To
address
this,
we
profiled
gene
expression
at
depth
in
metaphase
II
oocytes
bipronuclear
(2PN)
one-cell
embryos.
High-resolution
single-cell
RNA
sequencing
revealed
previously
inaccessible
oocyte-to-embryo
changes.
This
confirmed
transcript
depletion
following
fertilization
(maternal
degradation)
also
uncovered
low-magnitude
upregulation
hundreds
spliced
transcripts.
Gene
analysis
predicted
embryonic
processes
including
cell-cycle
progression
chromosome
maintenance
as
well
transcriptional
activators
that
included
cancer-associated
regulators.
Transcription
was
disrupted
abnormal
monopronuclear
(1PN)
tripronuclear
(3PN)
These
findings
indicate
initiates
sooner
than
thought.
The
pattern
promises
to
illuminate
involved
onset
development,
with
implications
for
epigenetic
inheritance,
stem-cell-derived
cancer.
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: Aug. 26, 2020
Abstract
While
footprinting
analysis
of
ATAC-seq
data
can
theoretically
enable
investigation
transcription
factor
(TF)
binding,
the
lack
a
computational
tool
able
to
conduct
different
levels
has
so-far
hindered
widespread
application
this
method.
Here
we
present
TOBIAS,
comprehensive,
accurate,
and
fast
framework
enabling
genome-wide
TF
binding
dynamics
for
hundreds
TFs
simultaneously.
We
validate
TOBIAS
using
paired
ChIP-seq
data,
find
that
outperforms
existing
methods
bias
correction
footprinting.
As
proof-of-concept,
illustrate
how
unveil
complex
during
zygotic
genome
activation
in
both
humans
mice,
propose
Dux
activates
cascades
TFs,
binds
repeat
elements
induces
expression
novel
genetic
elements.
Development,
Journal Year:
2019,
Volume and Issue:
146(11)
Published: June 1, 2019
The
development
of
animal
embryos
is
initially
directed
by
maternal
gene
products.
Then,
during
the
maternal-to-zygotic
transition
(MZT),
developmental
control
handed
to
zygotic
genome.
Extensive
research
in
both
vertebrate
and
invertebrate
model
organisms
has
revealed
that
MZT
can
be
subdivided
into
two
phases,
which
very
different
modes
regulation
are
implemented:
initially,
exclusively
post-transcriptional
post-translational,
following
gradual
activation
genome
leads
predominance
transcriptional
regulation.
These
changes
expression
program
precisely
controlled
highly
interconnected.
Here,
we
review
current
understanding
mechanisms
underlie
handover
MZT.
Science,
Journal Year:
2019,
Volume and Issue:
365(6451), P. 353 - 360
Published: July 4, 2019
Histone
modifications
regulate
gene
expression
and
development.
To
address
how
they
are
reprogrammed
in
human
early
development,
we
investigated
key
histone
marks
oocytes
embryos.
Unlike
that
mouse
oocytes,
the
permissive
mark
trimethylated
H3
lysine
4
(H3K4me3)
largely
exhibits
canonical
patterns
at
promoters
oocytes.
After
fertilization,
prezygotic
genome
activation
(pre-ZGA)
embryos
acquire
chromatin
widespread
H3K4me3
CpG-rich
regulatory
regions.
By
contrast,
repressive
H3K27me3
undergoes
global
depletion.
regions
then
resolve
to
either
active
or
repressed
states
upon
ZGA,
followed
by
subsequent
restoration
of
developmental
genes.
Finally,
combining
transcriptome
maps,
revealed
transcription
circuitry
asymmetric
patterning
during
lineage
specification.
Collectively,
our
data
unveil
a
priming
phase
connecting
parental-to-zygotic
epigenetic
transition.
Science,
Journal Year:
2018,
Volume and Issue:
361(6409), P. 1332 - 1336
Published: Sept. 28, 2018
During
development
and
throughout
life,
a
variety
of
specialized
cells
must
be
generated
to
ensure
the
proper
function
each
tissue
organ.
Chromatin
plays
key
role
in
determining
cellular
state,
whether
totipotent,
pluripotent,
multipotent,
or
differentiated.
We
highlight
chromatin
dynamics
involved
generation
pluripotent
stem
as
well
their
influence
on
cell
fate
decision
reprogramming.
focus
capacity
histone
variants,
chaperones,
modifications,
heterochromatin
factors
identity
its
plasticity.
Recent
technological
advances
have
provided
tools
elucidate
underlying
for
better
understanding
normal
pathological
conditions,
with
avenues
potential
therapeutic
application.
Protein & Cell,
Journal Year:
2020,
Volume and Issue:
12(1), P. 7 - 28
Published: July 15, 2020
Mammalian
fertilization
begins
with
the
fusion
of
two
specialized
gametes,
followed
by
major
epigenetic
remodeling
leading
to
formation
a
totipotent
embryo.
During
development
pre-implantation
embryo,
precise
reprogramming
progress
is
prerequisite
for
avoiding
developmental
defects
or
embryonic
lethality,
but
underlying
molecular
mechanisms
remain
elusive.
For
past
few
years,
unprecedented
breakthroughs
have
been
made
in
mapping
regulatory
network
dynamic
epigenomes
during
mammalian
early
embryo
development,
taking
advantage
multiple
advances
and
innovations
low-input
genome-wide
chromatin
analysis
technologies.
The
aim
this
review
highlight
most
recent
understanding
embryogenesis
mammals,
including
DNA
methylation,
histone
modifications,
accessibility
3D
organization.
