Molecular topography of an entire nervous system

Cell, Journal Year: 2021, Volume and Issue: 184(16), P. 4329 - 4347.e23

Published: July 7, 2021

Language: Английский

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What is a cell type and how to define it?

Cell, Journal Year: 2022, Volume and Issue: 185(15), P. 2739 - 2755

Published: July 1, 2022

Language: Английский

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A connectomic study of a petascale fragment of human cerebral cortex

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: May 30, 2021

Abstract We acquired a rapidly preserved human surgical sample from the temporal lobe of cerebral cortex. stained 1 mm 3 volume with heavy metals, embedded it in resin, cut more than 5000 slices at ∼30 nm and imaged these sections using high-speed multibeam scanning electron microscope. used computational methods to render three-dimensional structure containing 57,216 cells, hundreds millions neurites 133.7 million synaptic connections. The 1.4 petabyte microscopy volume, segmented cell parts, blood vessels, myelin, inhibitory excitatory synapses, 104 manually proofread cells are available peruse online . Many interesting unusual features were evident this dataset. Glia outnumbered neurons 2:1 oligodendrocytes most common type volume. Excitatory spiny comprised 69% neuronal population, synapses also majority (76%). drive onto was biased strongly toward excitation (70%) case for interneurons (48%). Despite incompleteness automated segmentation caused by split merge errors, we could automatically generate (and then validate) connections between neuron types both within layers. In studying found that deep layer can be classified into new subsets, based on structural connectivity differences, chandelier not only innervate initial segments as previously described, but each other’s segments. Furthermore, among thousands weak established neuron, there exist rarer highly powerful axonal inputs establish multi-synaptic contacts (up ∼20 synapses) target neurons. Our analysis indicates strong specific, allow small numbers axons have an outsized role activity some their postsynaptic partners.

Language: Английский

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The connectome of an insect brain

Science, Journal Year: 2023, Volume and Issue: 379(6636)

Published: March 10, 2023

Brains contain networks of interconnected neurons and so knowing the network architecture is essential for understanding brain function. We therefore mapped synaptic-resolution connectome an entire insect ( Drosophila larva) with rich behavior, including learning, value computation, action selection, comprising 3016 548,000 synapses. characterized neuron types, hubs, feedforward feedback pathways, as well cross-hemisphere brain-nerve cord interactions. found pervasive multisensory interhemispheric integration, highly recurrent architecture, abundant from descending neurons, multiple novel circuit motifs. The brain’s most circuits comprised input output learning center. Some structural features, multilayer shortcuts nested loops, resembled state-of-the-art deep architectures. identified provides a basis future experimental theoretical studies neural circuits.

Language: Английский

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Large-scale neural recordings call for new insights to link brain and behavior

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(1), P. 11 - 19

Published: Jan. 1, 2022

Language: Английский

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Neuronal wiring diagram of an adult brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Language: Английский

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Volume electron microscopy

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: July 7, 2022

Language: Английский

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A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution

Science, Journal Year: 2024, Volume and Issue: 384(6696)

Published: May 9, 2024

To fully understand how the human brain works, knowledge of its structure at high resolution is needed. Presented here a computationally intensive reconstruction ultrastructure cubic millimeter temporal cortex that was surgically removed to gain access an underlying epileptic focus. It contains about 57,000 cells, 230 millimeters blood vessels, and 150 million synapses comprises 1.4 petabytes. Our analysis showed glia outnumber neurons 2:1, oligodendrocytes were most common cell, deep layer excitatory could be classified on basis dendritic orientation, among thousands weak connections each neuron, there exist rare powerful axonal inputs up 50 synapses. Further studies using this resource may bring valuable insights into mysteries brain.

Language: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 139 - 152

Published: Oct. 2, 2024

Language: Английский

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The neuropeptidergic connectome of C. elegans

Neuron, Journal Year: 2023, Volume and Issue: 111(22), P. 3570 - 3589.e5

Published: Nov. 1, 2023

Efforts are ongoing to map synaptic wiring diagrams, or connectomes, understand the neural basis of brain function. However, chemical synapses represent only one type functionally important neuronal connection; in particular, extrasynaptic, "wireless" signaling by neuropeptides is widespread and plays essential roles all nervous systems. By integrating single-cell anatomical gene-expression datasets with biochemical analysis receptor-ligand interactions, we have generated a draft connectome neuropeptide C. elegans system. This network characterized high connection density, extended cascades, autocrine foci, decentralized topology, large, highly interconnected core containing three constituent communities sharing similar patterns input connectivity. Intriguingly, several key hubs little-studied neurons that appear specialized for peptidergic neuromodulation. We anticipate neuropeptidergic will serve as prototype how networks neuromodulatory organized.

Language: Английский

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