Neuro-Oncology Advances,
Journal Year:
2025,
Volume and Issue:
7(1)
Published: Jan. 1, 2025
Abstract
Background
The
reactivation
of
neurodevelopmental
programs
in
cancer
highlights
parallel
biological
processes
that
occur
both
normal
development
and
brain
tumors.
Achieving
a
deeper
understanding
how
dysregulated
developmental
factors
play
role
the
progression
tumors
is
therefore
crucial
for
identifying
potential
targets
therapeutic
interventions.
Single-cell
RNA-sequencing
(scRNA-Seq)
provides
an
opportunity
to
understand
are
reinitiated
at
single-cell
resolution.
aim
this
study
identify
origins
using
scRNA-Seq
data.
Methods
Here,
we
introduce
COORS
(Cell
Of
ORigin
like
CellS),
computational
tool
trained
on
human
datasets
annotates
“developmental-like”
cell
states
leverages
type-specific
multilayer
perceptron
models
incorporates
type
tree
reflects
hierarchical
relationships
probabilities.
Results
Applying
various
datasets,
including
medulloblastoma
(MB),
glioma,
diffuse
midline
glioma
(DMG),
identified
developmental-like
cells
represent
putative
origin
these
Our
method
classification
age
regression,
offering
insights
into
types
tumor
subgroups.
outer
radial
glia
within
IDHWT
whereas
oligodendrocyte
precursor
(OPCs)
neuronal-like
IDHMut.
Interestingly,
IDHMut
subgroup
map
OPC
show
bimodal
distributions
early
late
weeks
development.
Furthermore,
offers
valuable
resource
by
providing
novel
markers
linked
MB,
DMG
Conclusions
work
adds
our
cumulative
heterogeneity
helps
pave
way
tailored
treatment
strategies.
Cell stem cell,
Journal Year:
2023,
Volume and Issue:
30(6), P. 851 - 866.e7
Published: May 15, 2023
The
emergence
of
the
three
germ
layers
and
lineage-specific
precursor
cells
orchestrating
organogenesis
represent
fundamental
milestones
during
early
embryonic
development.
We
analyzed
transcriptional
profiles
over
400,000
from
14
human
samples
collected
post-conceptional
weeks
(PCW)
3
to
12
delineate
dynamic
molecular
cellular
landscape
gastrulation
nervous
system
described
diversification
cell
types,
spatial
patterning
neural
tube
cells,
signaling
pathways
likely
involved
in
transforming
epiblast
into
neuroepithelial
then
radial
glia.
resolved
24
clusters
glial
along
outlined
differentiation
trajectories
for
main
classes
neurons.
Lastly,
we
identified
conserved
distinctive
features
across
species
by
comparing
single-cell
transcriptomic
between
humans
mice.
This
comprehensive
atlas
sheds
light
on
mechanisms
underlying
brain
Neuro-Oncology,
Journal Year:
2023,
Volume and Issue:
25(10), P. 1895 - 1909
Published: Aug. 3, 2023
Abstract
Background
Distinguishing
the
cellular
origins
of
childhood
brain
tumors
is
key
for
understanding
tumor
initiation
and
identifying
lineage-restricted,
tumor-specific
therapeutic
targets.
Previous
strategies
to
map
cell-of-origin
typically
involved
comparing
human
murine
embryonal
tissues,
which
potentially
limited
due
species-specific
differences.
The
aim
this
study
was
unravel
3
most
common
pediatric
tumors,
ependymoma,
pilocytic
astrocytoma,
medulloblastoma,
using
a
developing
cerebellar
atlas.
Methods
We
used
single-nucleus
atlas
normal
cerebellum
consisting
176
645
cells
as
reference
an
in-depth
comparison
4416
bulk
single-cell
transcriptome
datasets,
gene
set
variation
analysis,
correlation,
matching
techniques.
Results
find
that
astroglial
lineage
origin
posterior
fossa
ependymomas.
propose
infratentorial
astrocytomas
originate
from
oligodendrocyte
MHC
II
genes
are
specifically
enriched
in
these
tumors.
confirm
SHH
Group
3/4
medulloblastomas
granule
cell
unipolar
brush
lineages.
Radiation-induced
gliomas
stem
glial
lineages
demonstrate
distinct
primary
medulloblastoma.
identify
expressed
origin,
specific;
both
sets
represent
promising
targets
future
study.
Conclusion
Based
on
our
results,
individual
within
may
resemble
different
types
along
restricted
developmental
lineage.
Therefore,
we
suggest
can
arise
multiple
states
“lineage
origin.”
Oncogene,
Journal Year:
2024,
Volume and Issue:
43(12), P. 839 - 850
Published: Feb. 14, 2024
Abstract
Medulloblastoma
is
one
of
the
most
common
malignant
pediatric
brain
tumors
derived
from
posterior
fossa.
The
current
treatment
includes
maximal
safe
surgical
resection,
radiotherapy,
whole
cranio-spinal
radiation
and
adjuvant
with
chemotherapy.
However,
it
can
only
limitedly
prolong
survival
time
severe
side
effects
relapse.
Defining
intratumoral
heterogeneity,
cellular
origin
identifying
interaction
network
within
tumor
microenvironment
are
helpful
for
understanding
mechanisms
medulloblastoma
tumorigenesis
Due
to
technological
limitations,
heterogeneity
have
not
been
fully
understood.
Recently,
emergence
single-cell
technology
has
provided
a
powerful
tool
achieving
goal
tumorigenesis.
Several
studies
demonstrated
each
subtype
utilizing
RNA-seq,
which
uncovered
before
using
conventional
technologies.
In
this
review,
we
present
an
overview
progress
in
discuss
novel
findings
age
