A faecal microbiota signature with high specificity for pancreatic cancer DOI Creative Commons
Ece Kartal, Thomas Schmidt, Esther Molina‐Montes

et al.

Gut, Journal Year: 2022, Volume and Issue: 71(7), P. 1359 - 1372

Published: March 8, 2022

Recent evidence suggests a role for the microbiome in pancreatic ductal adenocarcinoma (PDAC) aetiology and progression.To explore faecal salivary microbiota as potential diagnostic biomarkers.We applied shotgun metagenomic 16S rRNA amplicon sequencing to samples from Spanish case-control study (n=136), including 57 cases, 50 controls, 29 patients with chronic pancreatitis discovery phase, German (n=76), validation phase.Faecal classifiers performed much better than saliva-based identified PDAC an accuracy of up 0.84 area under receiver operating characteristic curve (AUROC) based on set 27 microbial species, consistent across early late disease stages. Performance further improved 0.94 AUROC when we combined our microbiome-based predictions serum levels carbohydrate antigen (CA) 19-9, only current non-invasive, Food Drug Administration approved, low specificity biomarker. Furthermore, microbiota-based classification model confined PDAC-enriched species was highly disease-specific validated against 25 publicly available populations various health conditions (n=5792). Both models had high prediction population (n=76). Several marker were detectable tumour non-tumour tissue using fluorescence situ hybridisation.Taken together, results indicate that robust specific screening detection is feasible.

Language: Английский

Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3 DOI Creative Commons
Francesco Beghini, Lauren J. McIver, Aitor Blanco‐Míguez

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: May 4, 2021

Culture-independent analyses of microbial communities have progressed dramatically in the last decade, particularly due to advances methods for biological profiling via shotgun metagenomics. Opportunities improvement continue accelerate, with greater access multi-omics, reference genomes, and strain-level diversity. To leverage these, we present bioBakery 3, a set integrated, improved taxonomic, strain-level, functional, phylogenetic metagenomes newly developed build on largest sequences now available. Compared current alternatives, MetaPhlAn 3 increases accuracy taxonomic profiling, HUMAnN improves that functional potential activity. These detected novel disease-microbiome links applications CRC (1262 metagenomes) IBD (1635 817 metatranscriptomes). Strain-level an additional 4077 StrainPhlAn PanPhlAn unraveled structure common gut microbe Ruminococcus bromii , previously described by only 15 isolate genomes. With open-source implementations cloud-deployable reproducible workflows, platform can help researchers deepen resolution, scale, multi-omic community studies.

Language: Английский

Citations

1417
Local and systemic mechanisms linking periodontal disease and inflammatory comorbidities DOI Open Access
George Hajishengallis, Triantafyllos Chavakis

Nature reviews. Immunology, Journal Year: 2021, Volume and Issue: 21(7), P. 426 - 440

Published: Jan. 28, 2021

Language: Английский

Citations

1032
Gut microbiota in colorectal cancer: mechanisms of action and clinical applications DOI
Sunny H. Wong, Jun Yu

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2019, Volume and Issue: 16(11), P. 690 - 704

Published: Sept. 25, 2019

Language: Английский

Citations

995
The microbiome and human cancer DOI
Gregory D. Sepich‐Poore, Laurence Zitvogel, Ravid Straussman

et al.

Science, Journal Year: 2021, Volume and Issue: 371(6536)

Published: March 25, 2021

Microbial roles in cancer formation, diagnosis, prognosis, and treatment have been disputed for centuries. Recent studies provocatively claimed that bacteria, viruses, and/or fungi are pervasive among cancers, key actors immunotherapy, engineerable to treat metastases. Despite these findings, the number of microbes known directly cause carcinogenesis remains small. Critically evaluating building frameworks such evidence light modern biology is an important task. In this Review, we delineate between causal complicit trace common themes their influence through host's immune system, herein defined as immuno-oncology-microbiome axis. We further review intratumoral approaches manipulate gut or tumor microbiome while projecting next phase experimental discovery.

Language: Английский

Citations

895
Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer DOI Creative Commons
Mingyang Song, Andrew T. Chan, Jun Sun

et al.

Gastroenterology, Journal Year: 2019, Volume and Issue: 158(2), P. 322 - 340

Published: Oct. 3, 2019

Language: Английский

Citations

605
Rising incidence of early-onset colorectal cancer — a call to action DOI
Naohiko Akimoto, Tomotaka Ugai, Rong Zhong

et al.

Nature Reviews Clinical Oncology, Journal Year: 2020, Volume and Issue: 18(4), P. 230 - 243

Published: Nov. 20, 2020

Language: Английский

Citations

464
The rising tide of early-onset colorectal cancer: a comprehensive review of epidemiology, clinical features, biology, risk factors, prevention, and early detection DOI
Swati Patel, Jordan J. Karlitz, Timothy Yen

et al.

˜The œLancet. Gastroenterology & hepatology, Journal Year: 2022, Volume and Issue: 7(3), P. 262 - 274

Published: Jan. 26, 2022

Language: Английский

Citations

451
Gut microbiota-derived bile acids in intestinal immunity, inflammation, and tumorigenesis DOI Creative Commons
Jie Cai, Lulu Sun, Frank J. Gonzalez

et al.

Cell Host & Microbe, Journal Year: 2022, Volume and Issue: 30(3), P. 289 - 300

Published: March 1, 2022

Language: Английский

Citations

451
The Intestinal Microbiota and Colorectal Cancer DOI Creative Commons
Yiwen Cheng, Zongxin Ling, Lanjuan Li

et al.

Frontiers in Immunology, Journal Year: 2020, Volume and Issue: 11

Published: Nov. 30, 2020

The intestinal microbiota, composed of a large population microorganisms, is often considered "forgotten organ" in human health and diseases. Increasing evidence indicates that dysbiosis the microbiota closely related to colorectal cancer (CRC). roles for microorganisms initiated facilitated CRC process are becoming increasingly clear. Hypothesis models have been proposed illustrate complex relationship between CRC. Recent studies identified Streptococcus bovis, enterotoxigenic Bacteroides fragilis, Fusobacterium nucleatum, Enterococcus faecalis, Escherichia coli, Peptostreptococcus anaerobius as candidate pathogens. In this review, we summarized mechanisms involved microbiota-related carcinogenesis, including inflammation, pathogenic bacteria, their virulence factors, genotoxins, oxidative stress, bacterial metabolites, biofilm. We also described clinical values novel strategies preventing treating

Language: Английский

Citations

408
Small molecule metabolites: discovery of biomarkers and therapeutic targets DOI Creative Commons
Shi Qiu, Ying Cai, Hong Yao

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: March 20, 2023

Metabolic abnormalities lead to the dysfunction of metabolic pathways and metabolite accumulation or deficiency which is well-recognized hallmarks diseases. Metabolite signatures that have close proximity subject's phenotypic informative dimension, are useful for predicting diagnosis prognosis diseases as well monitoring treatments. The lack early biomarkers could poor serious outcomes. Therefore, noninvasive methods with high specificity selectivity desperately needed. Small molecule metabolites-based metabolomics has become a specialized tool biomarker pathway analysis, revealing possible mechanisms human various deciphering therapeutic potentials. It help identify functional related variation delineate biochemical changes indicators pathological damage prior disease development. Recently, scientists established large number profiles reveal underlying networks target exploration in biomedicine. This review summarized analysis on potential value small-molecule candidate metabolites clinical events, may better diagnosis, prognosis, drug screening treatment. We also discuss challenges need be addressed fuel next wave breakthroughs.

Language: Английский

Citations

388