Recent Advances in 2D Material Theory, Synthesis, Properties, and Applications

ACS Nano, Journal Year: 2023, Volume and Issue: 17(11), P. 9694 - 9747

Published: May 23, 2023

Two-dimensional (2D) material research is rapidly evolving to broaden the spectrum of emergent 2D systems. Here, we review recent advances in theory, synthesis, characterization, device, and quantum physics materials their heterostructures. First, shed insight into modeling defects intercalants, focusing on formation pathways strategic functionalities. We also machine learning for synthesis sensing applications materials. In addition, highlight important development processing, characterization various (e.g., MXnenes, magnetic compounds, epitaxial layers, low-symmetry crystals, etc.) discuss oxidation strain gradient engineering Next, optical phonon properties controlled by inhomogeneity give examples multidimensional imaging biosensing equipped with analysis based platforms. then provide updates mix-dimensional heterostructures using building blocks next-generation logic/memory devices anomalous Hall high-quality topological insulators, followed small twist-angle homojunctions exciting transport. Finally, perspectives future work several topics mentioned this review.

Language: Английский

---

Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 29, 2022

Abstract Fast, inexpensive, and multiplexed detection of multiple nucleic acids is great importance to human health, yet it still represents a significant challenge. Herein, we propose acid testing platform, named MiCaR, which couples mi crofluidic device with CRISPR- C as12a nd multiplex r ecombinase polymerase amplification. With only one fluorescence probe, MiCaR can simultaneously test up 30 targets through microfluidic space coding. The limit achieves 0.26 attomole, the assay takes 40 min. We demonstrate utility by efficiently detecting nine HPV subtypes targeted 9-valent vaccine, showing sensitivity 97.8% specificity 98.1% in 100 patient samples at risk for infection. Additionally, also show generalizability our approach successfully eight most clinically relevant respiratory viruses. anticipate this effective, undecorated versatile platform be widely used detection.

Language: Английский

---

Two Years into the COVID-19 Pandemic: Lessons Learned

ACS Infectious Diseases, Journal Year: 2022, Volume and Issue: 8(9), P. 1758 - 1814

Published: Aug. 8, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and virulent human-infecting that emerged in late December 2019 Wuhan, China, causing disease called (COVID-19), which has massively impacted global public health caused widespread disruption to daily life. The crisis by COVID-19 mobilized scientists authorities across the world rapidly improve our knowledge about this devastating disease, shedding light on its management control, spawned development of new countermeasures. Here we provide an overview state art gained last years virus COVID-19, including origin natural reservoir hosts, viral etiology, epidemiology, modes transmission, clinical manifestations, pathophysiology, diagnosis, treatment, prevention, emerging variants, vaccines, highlighting important differences from previously known pathogenic coronaviruses. We also discuss selected key discoveries each topic underline gaps for future investigations.

Language: Английский

---

CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics

Nature Nanotechnology, Journal Year: 2023, Volume and Issue: 18(7), P. 798 - 807

Published: April 24, 2023

Synthetic biomarkers, bioengineered sensors that generate molecular reporters in diseased microenvironments, represent an emerging paradigm precision diagnostics. Despite the utility of DNA barcodes as a multiplexing tool, their susceptibility to nucleases vivo has limited utility. Here we exploit chemically stabilized nucleic acids multiplex synthetic biomarkers and produce diagnostic signals biofluids can be 'read out' via CRISPR nucleases. The strategy relies on microenvironmental endopeptidase trigger release acid polymerase-amplification-free, CRISPR-Cas-mediated barcode detection unprocessed urine. Our data suggest DNA-encoded nanosensors non-invasively detect differentiate disease states transplanted autochthonous murine cancer models. We also demonstrate CRISPR-Cas amplification harnessed convert readout point-of-care paper tool. Finally, employ microfluidic platform for densely multiplexed, CRISPR-mediated potentially evaluate complex human diseases rapidly guide therapeutic decisions.

Language: Английский

---

Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens

Nature Nanotechnology, Journal Year: 2023, Volume and Issue: 18(8), P. 922 - 932

Published: June 1, 2023

Language: Английский

---

Coupling CRISPR/Cas12a and Recombinase Polymerase Amplification on a Stand-Alone Microfluidics Platform for Fast and Parallel Nucleic Acid Detection

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3379 - 3389

Published: Feb. 3, 2023

Timely identification of human papillomavirus (HPV) infection is crucial for the prevention cervical cancer. Current HPV detection methods mainly rely on polymerase chain reaction (PCR), which often requires bulky equipment and a long assay time. In this work, we report heating-membrane-assisted multiplexed microfluidics platform that couples recombinase amplification (RPA) CRISPR technology (termed M3-CRISPR) fast low-cost multiple subtypes. The heating membrane can provide convenient temperature control on-chip RPA assays. This stand-alone system allows simultaneous HPV16 HPV18 with high specificity sensitivity (0.5 nM 1 × 10–18 M unamplified amplified plasmids, respectively) in 30 min fluorescence-based readout. Furthermore, introduced an optimized lateral flow dipstick (LFD) into portable to allow visualized DNA. LFD-based readout also reached plasmids realized successful subtypes clinical samples. Finally, established automatic microfluidic enables sample-in-answer-out We believe fast, convenient, affordable molecular diagnostic serve as useful tool point-of-care testing or other pathogens.

Language: Английский

---

Computer Vision-Based Artificial Intelligence-Mediated Encoding-Decoding for Multiplexed Microfluidic Digital Immunoassay

ACS Nano, Journal Year: 2023, Volume and Issue: 17(14), P. 13700 - 13714

Published: July 17, 2023

Digital immunoassays with multiplexed capacity, ultrahigh sensitivity, and broad affordability are urgently required in clinical diagnosis, food safety, environmental monitoring. In this work, a multidimensional digital immunoassay has been developed through microparticle-based encoding artificial intelligence-based decoding, enabling detection high sensitivity convenient operation. The information encoded the features of microspheres, including their size, number, color, allows for simultaneous identification accurate quantification multiple targets. Computer vision-based intelligence can analyze microscopy images decoding output results visually. Moreover, optical imaging be well integrated microfluidic platform, allowing encoding-decoding computer intelligence. This simultaneously inflammatory markers antibiotics within 30 min range from pg/mL to μg/mL, which holds great promise as an intelligent bioassay next-generation biosensing.

Language: Английский

---

Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet Device Enables Simultaneous Detection of HPV16 and HPV18

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3476 - 3485

Published: Feb. 1, 2023

Fast, simplified, and multiplexed detection of human papillomaviruses (HPVs) is great importance for both clinical management population screening. However, current HPV methods often require sophisticated instruments laborious procedures to detect multiple targets. In this work, we developed a simple microfluidic dual-droplet device (M-D3) the simultaneous HPV16 HPV18 by combining CRISPR-Cas12a system recombinase polymerase amplification (RPA) assay. A new approach pressure/vacuum was proposed efficient droplet generation with minimal sample consumption. Two groups droplets that separately encapsulate relevant Cas12a/crRNA fluorescent green or red reporters are parallelly generated, followed automatic imaging discriminate subtypes based on specific fluorescence droplets. The M-D3 platform performs high sensitivity (∼0.02 nM unamplified plasmids) specificity in detecting DNA. By RPA Cas12a assay, allows on-chip DNA simultaneously within 30 min, reaching limit 10–18 M (∼1 copy/reaction). Moreover, outstanding performance validated testing 20 patient samples infection risk, showing 92.3% 100%. integrating generator, CRISPR-Cas12a, RPA, provides an way two most harmful holds potential applications nucleic acid testing.

Language: Английский

---

Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(20)

Published: March 22, 2024

Abstract The CRISPR‐Cas12a system has emerged as a powerful tool for next‐generation nucleic acid‐based molecular diagnostics. However, it long been believed to be effective only on DNA targets. Here, we investigate the intrinsic RNA‐enabled trans‐ cleavage activity of AsCas12a and LbCas12a discover that they can directly activated by full‐size RNA targets, although exhibits weaker than both single‐stranded substrates. Remarkably, find RNA‐activated Cas12a possesses higher specificity in recognizing mutated target sequences compared activation. Based these findings, develop “ U niversal N uclease I dentification V irus E mpowered R NA‐ Se nsing” (UNIVERSE) assay acid testing. We incorporate T7 transcription step into this assay, thereby eliminating requirement protospacer adjacent motif (PAM) sequence target. Additionally, successfully detect multiple PAM‐less targets HIV clinical samples are undetectable conventional based double‐stranded activation, demonstrating unrestricted selection with UNIVERSE assay. further validate utility testing HPV 16 samples. envision targeting capability may bring paradigm shift Cas12a‐based detection enhance understanding CRISPR‐Cas biochemistry.

Language: Английский

---

Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 2, 2024

Abstract Real-time and continuous monitoring of nucleic acid biomarkers with wearable devices holds potential for personal health management, especially in the context pandemic surveillance or intensive care unit disease. However, achieving high sensitivity long-term stability remains challenging. Here, we report a tetrahedral nanostructure-based Natronobacterium gregoryi Argonaute (NgAgo) stable ultratrace unamplified acids (cell-free DNAs RNAs) vivo sepsis on device. This integrated wireless consists flexible circuit board, microneedle biosensor, stretchable epidermis patch enrichment capability. We comprehensively investigate recognition mechanism by NgAgo/guide DNA signal transformation within Debye distance. In experiments demonstrate suitability real-time cell-free RNA 0.3 fM up to 14 days. These results provide strategy highly sensitive molecular on-body detection acid.

Language: Английский

---