Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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Evolution of neuropeptide signalling systems

Journal of Experimental Biology, Journal Year: 2018, Volume and Issue: 221(3)

Published: Feb. 1, 2018

ABSTRACT Neuropeptides are a diverse class of neuronal signalling molecules that regulate physiological processes and behaviour in animals. However, determining the relationships evolutionary origins heterogeneous assemblage neuropeptides identified range phyla has presented huge challenge for comparative physiologists. Here, we review revolutionary insights into evolution neuropeptide have been obtained recently through analysis genome/transcriptome sequence data by ‘deorphanisation’ receptors. The at least 30 systems traced to common ancestor protostomes deuterostomes. Furthermore, two rounds genome duplication gave rise an expanded repertoire vertebrate lineage, enabling neofunctionalisation and/or subfunctionalisation, but with lineage-specific gene loss additional or duplications generating complex patterns phylogenetic distribution paralogous systems. We entering new era research where it become feasible compare roles orthologous wide phyla. Moreover, ambitious mission reconstruct function animal kingdom now represents tangible future.

Language: Английский

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The Neuropeptide Corazonin Controls Social Behavior and Caste Identity in Ants

Cell, Journal Year: 2017, Volume and Issue: 170(4), P. 748 - 759.e12

Published: Aug. 1, 2017

Language: Английский

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Systemic corazonin signalling modulates stress responses and metabolism inDrosophila

Open Biology, Journal Year: 2016, Volume and Issue: 6(11), P. 160152 - 160152

Published: Nov. 1, 2016

Stress triggers cellular and systemic reactions in organisms to restore homeostasis. For instance, metabolic stress, experienced during starvation, elicits a hormonal response that reallocates resources enable food search readjustment of physiology. Mammalian gonadotropin-releasing hormone (GnRH) its insect orthologue, adipokinetic (AKH), are known for their roles modulating stress-related behaviour. Here we show corazonin (Crz), peptide homologous AKH/GnRH, also alters stress physiology Drosophila . The Crz receptor (CrzR) is expressed salivary glands adipocytes the liver-like fat body, CrzR knockdown targeted simultaneously both these tissues increases fly's resistance desiccation oxidative reduces feeding, expression transcripts insulin-like peptides (DILPs), affects gene body. Furthermore, starved flies, CrzR-knockdown circulating stored carbohydrates. Thus, our findings indicate elevated signalling coordinates increased intake diminished energy stores regain Our study suggests an ancient stress-peptide Urbilateria evolved give rise present-day GnRH, AKH systems.

Language: Английский

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Synaptic and peptidergic connectome of a neurosecretory center in the annelid brain

eLife, Journal Year: 2017, Volume and Issue: 6

Published: Dec. 4, 2017

Neurosecretory centers in animal brains use peptidergic signaling to influence physiology and behavior. Understanding neurosecretory center function requires mapping cell types, synapses, networks. Here we transmission electron microscopy gene expression analyze the synaptic connectome of an entire center. We reconstructed 78 neurons mapped their connectivity brain larval Platynereis dumerilii, a marine annelid. These form anterior expressing many neuropeptides, including hypothalamic peptide orthologs receptors. Analysis peptide-receptor pairs spatially single-cell transcriptome data revealed sparsely connected networks linking specific neuronal subsets. experimentally analyzed one pair found that neuropeptide can couple signaling. Our study uncovered extensive within its connection brain.

Language: Английский

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The long and the short of it – a perspective on peptidergic regulation of circuits and behaviour

Journal of Experimental Biology, Journal Year: 2018, Volume and Issue: 221(3)

Published: Feb. 1, 2018

ABSTRACT Neuropeptides are the most diverse class of chemical modulators in nervous systems. They contribute to extensive modulation circuit activity and have profound influences on animal physiology. Studies invertebrate model organisms, including fruit fly Drosophila melanogaster nematode Caenorhabditis elegans, enabled genetic manipulation peptidergic signalling, contributing an understanding how neuropeptides pattern output neural circuits underpin behavioural adaptation. Electrophysiological pharmacological analyses well-defined microcircuits, such as crustacean stomatogastric ganglion, provided detailed insights into neuropeptide functions at a cellular level. These approaches can be increasingly applied mammalian brain by focusing with defined identifiable sub-population neurons. Functional systems been underpinned systematic studies map networks. Here, we review general principles mechanistic that emerged from these studies. We also highlight some challenges remain for furthering our functional relevance modulation.

Language: Английский

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The evolution and nomenclature of GnRH-type and corazonin-type neuropeptide signaling systems

General and Comparative Endocrinology, Journal Year: 2017, Volume and Issue: 264, P. 64 - 77

Published: June 14, 2017

Gonadotropin-releasing hormone (GnRH) was first discovered in mammals on account of its effect triggering pituitary release gonadotropins and the importance this discovery recognized forty years ago award 1977 Nobel Prize for Physiology or Medicine. Investigation evolution GnRH revealed that GnRH-type signaling systems occur throughout chordates, including agnathans (e.g. lampreys) urochordates sea squirts). Furthermore, adipokinetic (AKH) is ligand a receptor arthropod Drosophila melanogaster provided evidence antiquity signaling. However, occurrence other AKH-like peptides arthropods, which include corazonin AKH/corazonin-related peptide (ACP), has complicated efforts to reconstruct evolutionary history family related neuropeptides. Genome/transcriptome sequencing both receptors corazonin-type lophotrochozoan protostomes (annelids, mollusks) deuterostomian invertebrates (cephalochordates, hemichordates, echinoderms). act as ligands have been identified mollusks. what lacking experimental distinct peptide-receptor pathways deuterostomes. Importantly, we recently reported identification two neuropeptides either an echinoderm species – common European starfish Asterias rubens. Discovery invertebrate demonstrated time evolutionarily origin these paralogous can be traced ancestor lineage-specific losses (in vertebrates, nematodes) duplication system arthropods (giving rise AKH ACP systems) quadruplication vertebrates (followed by duplications) accounts phylogenetic distribution GnRH/corazonin-type extant animals. Informed new insights, here review research neuropeptide propose standardized nomenclature wherein are named “GnRH” “corazonin”, with exception arisen gene lineage referred “AKH” (or red pigment concentrating hormone, “RCPH”, crustaceans) “ACP”.

Language: Английский

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Discovery of novel representatives of bilaterian neuropeptide families and reconstruction of neuropeptide precursor evolution in ophiuroid echinoderms

Open Biology, Journal Year: 2017, Volume and Issue: 7(9), P. 170129 - 170129

Published: Sept. 1, 2017

Neuropeptides are a diverse class of intercellular signalling molecules that mediate neuronal regulation many physiological and behavioural processes. Recent advances in genome/transcriptome sequencing enabling identification neuropeptide precursor proteins species from growing variety animal taxa, providing new insights into the evolution signalling. Here, detailed analysis transcriptome sequence data three brittle star species, Ophionotus victoriae, Amphiura filiformis Ophiopsila aranea, has enabled first comprehensive precursors Ophiuroidea phylum Echinodermata. Representatives over 30 bilaterian families were identified, some which occur as paralogues. Furthermore, homologues endothelin/CCHamide, eclosion hormone, neuropeptide-F/Y nucleobinin/nesfatin discovered here deuterostome/echinoderm for time. The majority ophiuroid contain single copy neuropeptide, but several comprise multiple copies identical or non-identical, structurally related, neuropeptides. we performed an unprecedented investigation number period approximately 270 Myr by analysing 50 with reference to robust phylogeny. Our indicates composition 'cocktails' is functionally important, plasticity long evolutionary time scales.

Language: Английский

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Origin and Evolution of the Neuroendocrine Control of Reproduction in Vertebrates, With Special Focus on Genome and Gene Duplications

Physiological Reviews, Journal Year: 2019, Volume and Issue: 100(2), P. 869 - 943

Published: Oct. 18, 2019

In humans, as in the other mammals, neuroendocrine control of reproduction is ensured by brain-pituitary gonadotropic axis. Multiple internal and environmental cues are integrated via brain neuronal networks, ultimately leading to modulation activity gonadotropin-releasing hormone (GnRH) neurons. The decapeptide GnRH released into hypothalamic-hypophysial portal blood system stimulates production pituitary glycoprotein hormones, two gonadotropins luteinizing follicle-stimulating hormone. A novel actor, neuropeptide kisspeptin, acting upstream GnRH, has attracted increasing attention recent years. Other neuropeptides, such gonadotropin-inhibiting hormone/RF-amide related peptide, members RF-amide peptide superfamily, well various nonpeptidic neuromediators dopamine serotonin also provide a large panel stimulatory or inhibitory regulators. This paper addresses origin evolution vertebrate Brain-pituitary axes typical vertebrates, gland, mediator amplifier on peripheral organs, being innovation. reviews, from molecular functional perspectives, across radiation some key actors traces back their along lineage metazoa before emergence vertebrates. focus given how gene duplications, resulting either local events whole genome duplication events, followed paralogous loss conservation, might have shaped evolutionary scenarios current families

Language: Английский

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A neuroendocrine pathway modulating osmotic stress in Drosophila

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(3), P. e1009425 - e1009425

Published: March 8, 2021

Environmental factors challenge the physiological homeostasis in animals, thereby evoking stress responses. Various mechanisms have evolved to counter at organism level, including regulation by neuropeptides. In recent years, much progress has been made on and neuropeptides that regulate responses metabolic/nutritional stress, as well those involved countering osmotic ionic stresses. Here, we identified a peptidergic pathway links these types of regulatory functions. We uncover neuropeptide Corazonin (Crz), previously implicated metabolic neuroendocrine factor inhibits release diuretic hormone, CAPA, modulates tolerance stress. Both knockdown Crz acute injections peptide impact desiccation recovery from chill-coma. Mapping receptor ( CrzR ) expression three pairs Capa- expressing neurons (Va neurons) ventral nerve cord mediate effects Crz. show acts restore water/ion inhibiting CAPA via inhibition cAMP production Va neurons. Knockdown affects signaling, consequently increases for desiccation, starvation, but delays chill-coma recovery. Optogenetic activation stimulates excretion simultaneous CAPA-expressing reduces this response, supporting inhibitory action Thus, maintain homeostasis, which turn tolerance. Earlier work demonstrated systemic signaling restores nutrient levels promoting food search feeding. Here additionally propose also ensures suppressing diuresis. ameliorates stress-associated physiology through modulation both neurosecretory cells fat body Drosophila .

Language: Английский

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