Sorafenib-Loaded PLGA Carriers for Enhanced Drug Delivery and Cellular Uptake in Liver Cancer Cells DOI Creative Commons
Tania Mariastella Caputo, Angela Maria Cusano, Sofia Principe

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 4121 - 4142

Published: July 1, 2023

Introduction: Currently, conventional treatments of hepatocellular carcinoma (HCC) are not selective enough for tumor tissue and lead to multidrug resistance drug toxicity. Although sorafenib (SOR) is the standard first-line systemic therapy approved clinical treatment HCC, its poor aqueous solubility rapid clearance result in low absorption efficiency severely limit use local treatment. Methods: Herein, we present synthesis biodegradable polymeric Poly (D, L-Lactide-co-glycolide) (PLGA) particles loaded with SOR (PS) by emulsion-solvent evaporation process. The carefully characterized focusing on particle size, surface charge, morphology, loading content, encapsulation efficiency, vitro stability, release behaviour tested HepG2 cells. Additionally, PLGA have been coupled side emitting optical fibers ( se OF) integrated a microfluidic device light-triggered release. Results: PS size 248 nm, tunable charge uniform spherical shape without aggregation. shows 89.7% highest (8.9%) between SOR-loaded formulations. Treating cells containing at 7.5 μM their viability dampened 40%, 30% 17% after 48, 129 168 hours incubation, respectively. Conclusion: high sustained profile cellular uptake corroborate enhanced cytotoxicity effect HepG2. With prospect developing biomedical tools control spatial temporal drugs, successfully demonstrated potentiality OF carriers. Our prototypical system paves way new devices integrating microfluidics, fibers, advanced carriers capable deliver minimally invasive locoregional cancer treatments. Graphical Abstract: Keywords: poly(lactic-co-glycolic acid), sorafenib, emulsion solvent technique, carcinoma, fiber, microfluidics

Language: Английский

Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology DOI Creative Commons
Aleksandra Zielińska, Filipa Carreiró, Ana M. Oliveira

et al.

Molecules, Journal Year: 2020, Volume and Issue: 25(16), P. 3731 - 3731

Published: Aug. 15, 2020

Polymeric nanoparticles (NPs) are particles within the size range from 1 to 1000 nm and can be loaded with active compounds entrapped or surface-adsorbed onto polymeric core. The term “nanoparticle” stands for both nanocapsules nanospheres, which distinguished by morphological structure. NPs have shown great potential targeted delivery of drugs treatment several diseases. In this review, we discuss most commonly used methods production characterization NPs, association efficiency compound core, in vitro release mechanisms. As safety is a high priority, also toxicology ecotoxicology humans environment.

Language: Английский

Citations

1092
Recent advances in the formulation of PLGA microparticles for controlled drug delivery DOI Creative Commons
Elena Lagreca, Valentina Onesto, Concetta Di Natale

et al.

Progress in Biomaterials, Journal Year: 2020, Volume and Issue: 9(4), P. 153 - 174

Published: Oct. 15, 2020

Abstract Polymeric microparticles (MPs) are recognized as very popular carriers to increase the bioavailability and bio-distribution of both lipophilic hydrophilic drugs. Among different kinds polymers, poly-(lactic- co -glycolic acid) (PLGA) is one most accepted materials for this purpose, because its biodegradability (due presence ester linkages that degraded by hydrolysis in aqueous environments) safety (PLGA a Food Drug Administration (FDA)-approved compound). Moreover, depends on number glycolide units present structure, indeed, lower glycol content results an increased degradation time conversely higher monomer unit decreased time. Due feature, it possible design fabricate MPs with programmable time-controlled drug release. Many approaches procedures can be used prepare MPs. The chosen fabrication methodology influences size, stability, entrapment efficiency, release kinetics. For example, drugs chemotherapeutic agents (doxorubicin), anti-inflammatory non-steroidal (indomethacin), nutraceuticals (curcumin) were successfully encapsulated prepared single emulsion technique, while water-soluble compounds, such aptamer, peptides proteins, involved use double systems provide compartment prevent molecular degradation. purpose review overview about preparation characterization drug-loaded PLGA obtained single, microfluidic techniques, their current applications pharmaceutical industry. Graphic abstract

Language: Английский

Citations

205
PLGA-based nanomedicines manufacturing: Technologies overview and challenges in industrial scale-up DOI Creative Commons
Maria Camilla Operti, Alexander Bernhardt,

Silko Grimm

et al.

International Journal of Pharmaceutics, Journal Year: 2021, Volume and Issue: 605, P. 120807 - 120807

Published: June 16, 2021

Nanomedicines based on poly(lactic-co-glycolic acid) (PLGA) carriers offer tremendous opportunities for biomedical research. Although several PLGA-based systems have already been approved by both the Food and Drug Administration (FDA) European Medicine Agency (EMA), are widely used in clinics treatment or diagnosis of diseases, no PLGA nanomedicine formulation is currently available global market. One most impeding barriers development a manufacturing technique that allows transfer production from laboratory to an industrial scale with proper characterization quality control methods. This review provides comprehensive overview technologies analysis polymeric nanomedicines nanoparticles, scale-up challenges hinder their applicability, issues associated successful translation into clinical practice.

Language: Английский

Citations

181
Scale-up polymeric-based nanoparticles drug delivery systems: Development and challenges DOI Creative Commons
Yedi Herdiana, Nasrul Wathoni, Shaharum Shamsuddin

et al.

OpenNano, Journal Year: 2022, Volume and Issue: 7, P. 100048 - 100048

Published: May 28, 2022

The development of nanotechnology as a crucial technology in recent years has resulted new and creative nanomedicine applications. characteristics are determined by size, surface, drug loading, targeting potential combination with diagnostic agents for therapeutic applications to form nano multifunctional devices. Nanomedicine provides healthcare paradigm is capable revitalizing existing clinical products. Polymeric nanoparticles (PNPs) promising nanomedicines. With increasing demands medical, pharmaceutical, or nutritional applications, (NPs) require high reproducibility, homogeneity, control over their properties; upscaling processes unavoidable. Conventional small-scale laboratory synthesis techniques PNPs subject batch-to-batch variability. Increasing the installation size from scale industrial presents many difficulties. This review explores advances developing productivity systems synthesizing NPs an easy, fast, controlled manner challenges development, optimization, enhancement, feasibility, fabrication. There advantages use biocompatible biodegradable properties. Upscaling occur lot metal NPs, which can be reference PNPs; there similar PNPs.

Language: Английский

Citations

118
Recent Advances in the Surface Functionalization of PLGA-Based Nanomedicines DOI Creative Commons
Mazen M. El-Hammadi, José L. Arias

Nanomaterials, Journal Year: 2022, Volume and Issue: 12(3), P. 354 - 354

Published: Jan. 22, 2022

Therapeutics are habitually characterized by short plasma half-lives and little affinity for targeted cells. To overcome these challenges, nanoparticulate systems have entered into the disease arena. Poly(d,l-lactide-

Language: Английский

Citations

78
Approved Nanomedicine against Diseases DOI Creative Commons

Yuanchao Jia,

Yuxin Jiang,

Yonglong He

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(3), P. 774 - 774

Published: Feb. 26, 2023

Nanomedicine is a branch of medicine using nanotechnology to prevent and treat diseases. Nanotechnology represents one the most effective approaches in elevating drug‘s treatment efficacy reducing toxicity by improving drug solubility, altering biodistribution, controlling release. The development materials has brought profound revolution medicine, significantly affecting various major diseases such as cancer, injection, cardiovascular experienced explosive growth past few years. Although clinical transition nanomedicine not very satisfactory, traditional drugs still occupy dominant position formulation development, but increasingly active have adopted nanoscale forms limit side effects improve efficacy. review summarized approved nanomedicine, its indications, properties commonly used nanocarriers nanotechnology.

Language: Английский

Citations

59
The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles DOI Open Access
Antony V. Samrot, Sree Samanvitha Kuppa, Nagarajan Shobana

et al.

Polymers, Journal Year: 2021, Volume and Issue: 13(19), P. 3302 - 3302

Published: Sept. 27, 2021

Polyhydroxyalkanoates (PHAs) are storage granules found in bacteria that essentially hydroxy fatty acid polyesters. PHA molecules appear variety of structures, and amongst all types PHAs, polyhydroxybutyrate (PHB) is used versatile fields as it a biodegradable, biocompatible, ecologically safe thermoplastic. The unique physicochemical characteristics these PHAs have made them applicable nanotechnology, tissue engineering, other biomedical applications. In this review, the optimization, extraction, characterization described. Their production application nanotechnology also portrayed precise various methods PHA-based nanoparticles, such emulsion solvent diffusion, nanoprecipitation, dialysis discussed. techniques UV-Vis, FTIR, SEM, Zeta Potential, XRD elaborated.

Language: Английский

Citations

82
Pharmaceutical and drug delivery applications of chitosan biopolymer and its modified nanocomposite: A review DOI Creative Commons
Welela Meka Kedir,

Gamachu Fikadu Abdi,

Meta Mamo Goro

et al.

Heliyon, Journal Year: 2022, Volume and Issue: 8(8), P. e10196 - e10196

Published: Aug. 1, 2022

Due to their improved structural and functional properties as well biocompatibility, biodegradability, nontoxicity, chitosan its nanoparticles are currently grasping the interest of researchers. Although numerous attempts have been made apply derivatives biological applications, few reported in achieving pharmacological drug delivery. The goal current work is provide a summary biopolymer's physical, chemical, synthesis characterization modified nanocomposites. delivery method pharmaceutical applications biopolymer nanocomposites examined further detail this research. We will introduce also about most publications field study recent expansion.

Language: Английский

Citations

66
Quercetin against MCF7 and CAL51 breast cancer cell lines: apoptosis, gene expression and cytotoxicity of nano-quercetin DOI
Hamdoon A. Mohammed, Ghassan M. Sulaiman,

Sahar S. Anwar

et al.

Nanomedicine, Journal Year: 2021, Volume and Issue: 16(22), P. 1937 - 1961

Published: Aug. 25, 2021

Aims: To evaluate the anti breast-cancer activity, biocompatibility and toxicity of poly(d,l)-lactic-co-glycolic acid (PLGA)-encapsulated quercetin nanoparticles (Q-PLGA-NPs). Materials & methods: Quercetin was nano-encapsulated by an emulsion-diffusion process, were fully characterized through Fourier transform infrared spectroscopy, x-ray diffractions, FESEM zeta-sizer analysis. Activity against CAL51 MCF7 cell lines assessed DNA fragmentation assays, fluorescence microscopy, acridine-orange, propidium-iodide double-stainings. Biocompatibility towards red blood cells mice also explored. Results: The Q-PLGA-NPs exhibited apoptotic activity lines. murine in vivo studies showed no significant alterations liver kidney's functional biomarkers, apparent abnormalities, or tissue damages observed histological images liver, spleen, lungs, heart kidneys. Conclusion: study established preliminary vitro efficacy safety as a potential anti-breast cancer formulation.Lay abstract is flavonoid, type chemical, antioxidant nature, found many fruits vegetables. It known to have anticancer properties. In this study, encased into nano-sized particles biologically compatible bio-degradable synthetic polymer, named PLGA (poly-[D,L]-lactic-co-glycolic acid). effects nanoparticles/nano-quercetin tested two types breast laboratory. quercetin-loaded able kill cells, suggesting they could be body. Also, when given mice, did not appear damage any organ, change functions kidneys, thereby that are toxic. Further work required assess how well used treat people.

Language: Английский

Citations

65
Innovative Delivery Systems Loaded with Plant Bioactive Ingredients: Formulation Approaches and Applications DOI Creative Commons
Anastasia Kyriakoudi, Eleni Spanidi, Ioannis Mourtzinos

et al.

Plants, Journal Year: 2021, Volume and Issue: 10(6), P. 1238 - 1238

Published: June 18, 2021

Plants constitute a rich source of diverse classes valuable phytochemicals (e.g., phenolic acids, flavonoids, carotenoids, alkaloids) with proven biological activity antioxidant, anti-inflammatory, antimicrobial, etc.). However, factors such as low stability, poor solubility and bioavailability limit their food, cosmetics pharmaceutical applications. In this regard, wide range delivery systems have been developed to increase the stability plant-derived bioactive compounds upon processing, storage or under gastrointestinal digestion conditions, enhance solubility, mask undesirable flavors well efficiently deliver them target tissues where they can exert promote human health. present review, latest advances regarding design innovative for pure plant compounds, extracts essential oils, in order overcome above-mentioned challenges, are presented. Moreover, broad spectrum applications along future trends critically discussed.

Language: Английский

Citations

61