Pyrene-Based Light-Harvesting Antenna Molecules

The Journal of Physical Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown, P. 2468 - 2478

Published: Feb. 28, 2025

Light-harvesting antenna systems (AS) with multiple light-absorbing chromophores play a vital role in absorbing sunlight and transferring the excitation energy to reaction centers during photosynthesis process. Learning from nature, set of simple artificial pyrene-based light-harvesting have been designed re-examined self-developing chemical intermediates, via combining electron-donating 4,4-dimethoxy-triphenylamine moieties as for donors center. These exhibit positive correlation between molar absorption coefficient (ε), enhanced photoluminescence efficiency unchanged emission peak, two-photon cross-section an increasing number TPA-OMe solution. Moreover, excited-state dynamics these AS indicated that coexistence charge transfer (CT) state separation (CS) plays significant affecting behavior. The short-lived CS was affected by increased low polar solvent, which can boost decay recombination (CR), resulting emission. On contrary, long-lived would overwhelm CT high solvent or molecules containing one two units.

Language: Английский

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Dominant Analytical Techniques in DNA Nanotechnology for Various Applications

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(9), P. 3687 - 3697

Published: Feb. 14, 2024

DNA nanotechnology is rapidly gaining traction in numerous applications, each bearing varying degrees of tolerance to the quality and quantity necessary for viable nanostructure function. Despite distinct objectives application, they are united their reliance on essential analytical techniques, such as purification characterization. This tutorial aims guide reader through current state chemistry, outlining important factors consider when designing, assembling, purifying, characterizing a downstream applications.

Language: Английский

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Proximity-Induced Bipedal DNA Walker for Accurately Visualizing microRNA in Living Cancer Cell

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10669 - 10676

Published: June 24, 2024

DNA walker, a type of dynamic device that is capable moving progressively along prescribed walking tracks, has emerged as an ideal and powerful tool for biosensing bioimaging. However, most the reported three-dimensional (3D) walker were merely designed detection single target, they not achieving universal applicability. Herein, we first time development proximity-induced 3D bipedal imaging low abundance biomolecules. As proof concept, miRNA-34a, biomarker breast cancer, chosen model system to demonstrate this approach. In our design, can be generated only by specific recognition two proximity probes miRNA-34a. Meanwhile, it stochastically autonomously traveled on tracks (gold nanoparticles) via catalytic hairpin assembly (CHA), resulting in amplified fluorescence signal. comparison with some conventional walkers utilized living cell imaging, induced ligation assay greatly improve ensure high selectivity bioanalysis. By taking advantage these unique features, successfully realizes accurate effective monitoring target miRNA-34a expression levels cells, affording universal, valuable, promising platform low-abundance cancer identification cancer.

Language: Английский

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Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: Feb. 29, 2024

Peptide-based liquid-liquid phase separated domains, or coacervates, are a biomaterial gaining new interest due to their exciting potential in fields ranging from biosensing drug delivery. In this study, we demonstrate that coacervates provide simple and biocompatible medium improve nucleic acid biosensors through the sequestration of both biosensor target strands within coacervate, thereby increasing local concentration. Using well-established polyarginine (R

Language: Английский

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Sculpting photoproducts with DNA origami

Chem, Journal Year: 2024, Volume and Issue: 10(5), P. 1553 - 1575

Published: April 5, 2024

Language: Английский

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Single‐Molecule Assessment of DNA Hybridization Kinetics on Dye‐Loaded DNA Nanostructures

Small, Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract DNA nanostructures offer a versatile platform for precise dye assembly, making them promising templates creating photonic complexes with applications in photonics and bioimaging. However, despite these advancements, the effect of loading on hybridization kinetics single‐stranded protruding from remains unexplored. In this study, points accumulation imaging nanoscale topography (DNA‐PAINT) technique is employed to investigate accessibility functional binding sites DNA‐templated excitonic wires. The results indicate that positively charged dyes frameworks can accelerate protruded ssDNA through long‐range electrostatic interactions. Furthermore, impacts various are explored diverse varying cross‐sizes. research underscores crucial role interactions within DNA‐dye complexes, offering valuable insights functionalization assembly biomimetic systems.

Language: Английский

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Purification of DNA Nanoparticles Using Photocleavable Biotin Tethers

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(17), P. 22334 - 22343

Published: April 18, 2024

The number of applications self-assembled deoxyribonucleic acid (DNA) origami nanoparticles (DNA NPs) has increased drastically, following the development a variety single-stranded template DNA (ssDNA) that can serve as scaffold strand. In addition to viral genomes, such M13 bacteriophage and lambda DNAs, enzymatically produced ssDNA from various sources is rapidly gaining traction being applied for NP preparation. However, separating fully formed NPs have custom scaffolds crude assembly mixes often multistep process first its enzymatic amplification then isolating assembled excess precursor strands. Only sample ready downstream characterization application. this work, we highlight single-step purification sequence- or M13-derived scaffold-based using photocleavable biotin tethers. only requires an inexpensive ultraviolet (UV) lamp, with up 90% yield high purity are obtained. We show versatility in two multihelix bundle structures wireframe polyhedral architecture.

Language: Английский

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Soft Matter Photonics: Interplay of Soft Matter and Light

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

The light-soft matter interaction and its applications form the foundation of Soft Matter Photonics, here termed "Soft Mattonics", positioning it as fertile ground for developing next-generation photonic technologies. Over past few decades, this rapidly evolving field has achieved significant advancements, leading to successful across a wide range disciplines, including optoelectronics, photonics, information technology, material science, robotics, biomedicine, astronomy. In Perspective, we provide an overview Mattonics, highlighting recent developments in light-controlled soft their light manipulating. Additionally, offer insights into future research directions with emphasis on both foundational practical that will drive continued growth innovation field.

Language: Английский

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Distinguishing packing configurations of molecular dimers using excited-state absorption peaks in two-dimensional electronic spectra

The Journal of Chemical Physics, Journal Year: 2025, Volume and Issue: 162(17)

Published: May 1, 2025

Packing conformations of molecular aggregates are known to strongly influence the locations and intensities spectral peaks. Here, we develop third-order nonlinear spectroscopy signals for a purely electronic model dimer, which is prototype aggregate system. The model-which focuses on excited-state absorption (ESA) pathways in two-dimensional spectra-reveals that orientational averaging leads diagnostic ESA peak H- J-dimers. We constructed DNA-templated dimers cyanine molecules as representative systems used ultrabroadband measurements support predicted signatures arising from theoretical model. Fitting steady-state spectra supports assigned packing conformations. results elucidate how peaks can be conformation. This work lays foundation future studies include complicating effects vibronic states additional levels.

Language: Английский

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Characterizing Absolute Orientations in DNA Self-Assembly of Single Molecules

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

DNA self-assembly of single molecules (i.e., dyes) with deterministic orientations is a powerful approach for engineering molecular excitons. However, current determination methods the dye orientation relative to do not account plane, which missing degree freedom needed define absolute three-dimensional orientations. In this work, we combine fluorescence-detected linear dichroism, defocused dipole imaging, and point accumulation imaging in nanoscale topography (DNA-PAINT) super-resolution microscopy determine Cy5 dyes host duplexes, includes plane orientation. The data revealed that absorption emission dipoles are perpendicular duplex, mean parallel bases, supports notion intercalate between base pairs. presented methodology will inspire investigation controlling arrangement configurations beyond spontaneous π-stacking as well achieving novel dye-DNA arrangements.

Language: Английский

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