Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125906 - 125906
Published: March 11, 2024
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 207, P. 114167 - 114167
Published: March 17, 2022
Language: Английский
Citations
198
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: Oct. 29, 2022
Abstract Fast, inexpensive, and multiplexed detection of multiple nucleic acids is great importance to human health, yet it still represents a significant challenge. Herein, we propose acid testing platform, named MiCaR, which couples mi crofluidic device with CRISPR- C as12a nd multiplex r ecombinase polymerase amplification. With only one fluorescence probe, MiCaR can simultaneously test up 30 targets through microfluidic space coding. The limit achieves 0.26 attomole, the assay takes 40 min. We demonstrate utility by efficiently detecting nine HPV subtypes targeted 9-valent vaccine, showing sensitivity 97.8% specificity 98.1% in 100 patient samples at risk for infection. Additionally, also show generalizability our approach successfully eight most clinically relevant respiratory viruses. anticipate this effective, undecorated versatile platform be widely used detection.
Language: Английский
Citations
129
Analytica Chimica Acta, Journal Year: 2021, Volume and Issue: 1185, P. 338882 - 338882
Published: July 26, 2021
Language: Английский
Citations
109
Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(27), P. 9724 - 9731
Published: June 28, 2022
As a golden partner of recombinase polymerase amplification (RPA), CRISPR/Cas12a has been proven to solve the false-positive problem caused by nonspecific perfectly; meanwhile, its trans-cleave activity further enhanced sensitivity. However, solution transfer operation after tube cap opening greatly increases risk aerosol contamination amplicon, which is inconsistent with point-of-care (POC) diagnostics requirements. This study proposes photoactivated strategy achieve one-pot high-sensitivity nucleic acid detection. Using photocleavable complementary ssDNA block crRNA, RPA can smoothly pass through exponential interval without being affected activated Cas12a in critical early stage. After enough amplicons were produced, test was short bursts ultraviolet radiation at 365 nm. method achieved sensitivity 2.5 copies within 40 min. simple and sensitive effectively avoid amplicon lower threshold for molecular POC.
Language: Английский
Citations
98
Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(27), P. 9603 - 9609
Published: July 1, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection can be combined with recombinase-aided amplification (RAA) to enable rapid, accurate, and early of SARS-CoV-2. Current CRISPR-based approaches detecting viral typically require immense manual operations transfer RPA amplicons for CRISPR or suffer from compromised sensitivity by mixing the competing detection. Here, we develop dual-CRISPR/Cas12a-assisted RT-RAA assay a ″sample-to-answer″ centrifugal microfluidic platform that automatically detect 1 copy/μL SARS-CoV-2 within 30 min. This chip separates (CRISPR), such is maximized time consumption decreased factor 3. For 26 positive 8 negative clinical samples, this automated microfluidics achieved 100% accuracy compared gold-standard RT-PCR technique. point-of-care test, advantages being one-step, automated, sensitive, will have significant potential diagnosis disease prevention.
Language: Английский
Citations
74
Talanta, Journal Year: 2022, Volume and Issue: 243, P. 123388 - 123388
Published: March 12, 2022
Language: Английский
Citations
72
Sensors and Actuators A Physical, Journal Year: 2024, Volume and Issue: 376, P. 115625 - 115625
Published: June 24, 2024
The integration of CRISPR technology with microfluidic-based biosensors has greatly expanded its applications in medicine and molecular biology. This combination offers enhanced sensitivity selectivity medical diagnostics. CRISPR-powered microfluidics enables the accurate quantification DNA RNA associated diseases such as cancer, viral infections, bacterial diseases. precise targeting capabilities allow for detection specific genetic sequences, aiding early diagnosis, disease monitoring, treatment assessment. improves specificity by leveraging programmable nature coupling it signal readouts. However, challenges assay optimization, standardization, device fabrication need to be addressed. Additionally, complexity presents potential limitations terms off-target effects unintended modifications. Overall, holds tremendous diagnostics, but further research development are required fully exploit benefits clinical settings.
Language: Английский
Citations
20
Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2750 - 2750
Published: Nov. 30, 2024
The field of personalized medicine is undergoing a transformative shift through the integration multi-omics data, which mainly encompasses genomics, transcriptomics, proteomics, and metabolomics. This synergy allows for comprehensive understanding individual health by analyzing genetic, molecular, biochemical profiles. generation data enable more precise tailored therapeutic strategies, improving efficacy treatments reducing adverse effects. However, several challenges hinder full realization medicine. Key hurdles include complexity across different omics layers, need advanced computational tools, high cost generation. Additionally, issues related to privacy, standardization, robust validation in diverse populations remain significant obstacles. Looking ahead, future promises advancements technology methodologies that will address these challenges. Emerging innovations analytics, machine learning, high-throughput sequencing are expected enhance making accessible effective. Collaborative efforts among researchers, clinicians, industry stakeholders crucial overcoming fully harnessing potential individualized healthcare.
Language: Английский
Citations
18
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 17, 2025
Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia also probed explore influence formation on gene delivery and expression biochips. However, how affect transfection is unsettled underlying trafficking mechanism exogenous genes engineered single not announced. Therefore, microarray biochips were conceptualized prepared by synthesized photointelligent biopolymer precisely manage geometric topological structures (cell size protrusion) The cytoskeleton could be regulated large with more antennas assembled well-organized actin filaments cell tension distribution. stiffness adhesion force measured atomic microscope reveal nanomechanics Cytoskeleton-mediated adjusted filaments. Gene efficiency was enhanced increasing nanomechanics, which confirmed evaluation internalization capacity nanoparticles DNA synthesis ability. This work will provide a new strategy study functional biomaterials, chips internal patterned
Language: Английский
Citations
3
ACS Nano, Journal Year: 2021, Volume and Issue: 15(5), P. 7848 - 7859
Published: May 7, 2021
Clustered, regularly interspaced short palindromic repeats (CRISPR)-based diagnoses, derived from gene-editing technology, have been exploited for less than 5 years and are now reaching the stage of precommercial use. CRISPR tools some notable features, such as recognition at physiological temperature, excellent specificity, high-efficiency signal amplification capabilities. These characteristics promising development next-generation diagnostic technologies. In this Perspective, we present a detailed summary which micro/nanotechnologies play roles in advancement diagnosis how they involved. The use nanoprobes, nanochips, nanodevices, microfluidic lateral flow strips, etc. detection systems has led to new opportunities CRISPR-based assay development, achieving equipment-free detection, providing more compact systems, improving sensitivity quantitative Although tremendous progress made, not yet reached its full potential. We discuss upcoming improvements will continue key roles.
Language: Английский
Citations
88