CRISPR/Cas12a-Mediated Aptasensor Based on Tris-(8-hydroxyquinoline)aluminum Microcrystals with Crystallization-Induced Enhanced Electrochemiluminescence for Acetamiprid Analysis DOI

Xiaoge Peng,

Ying He,

Jinwen Zhao

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(26), P. 10068 - 10076

Published: June 20, 2023

Improving the electrochemiluminescence (ECL) efficiency of luminophores has always been goal ECL field. Herein, a novel crystallization-induced enhanced (CIE ECL) strategy was exploited to significantly enhance metal complex tris-(8-hydroxyquinoline)aluminum (Alq3). Alq3 monomers self-assembled and directionally grew form microcrystals (Alq3 MCs) in presence sodium dodecyl sulfate. The highly ordered crystal structure MCs not only constrained intramolecular rotation decrease nonradiative transition but also accelerated electron transfer between coreactant tripropylamine increase radiative transition, thus leading CIE effect. exhibited brilliant anode emission, which 210-fold stronger than that monomers. exceptional performance coupled efficient trans-cleavage activity CRISPR/Cas12a assisted by rolling circle amplification catalytic hairpin assembly fabricate CRISPR/Cas12a-mediated aptasensor for acetamiprid (ACE) detection. limit detection as low 0.79 fM. This work innovatively complexes integrated with dual ultrasensitive monitoring pesticides such ACE.

Language: Английский

Integrating CRISPR/Cas within isothermal amplification for point-of-Care Assay of nucleic acid DOI
Limei Zhang, Hui Jiang, Zixin Zhu

et al.

Talanta, Journal Year: 2022, Volume and Issue: 243, P. 123388 - 123388

Published: March 12, 2022

Language: Английский

Citations

72
A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters DOI
Long Ma, Junling Wang, Yaru Li

et al.

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 443, P. 130234 - 130234

Published: Oct. 27, 2022

Language: Английский

Citations

67
Improved Strategies for CRISPR-Cas12-based Nucleic Acids Detection DOI Open Access

Miao Qiu,

Xiaoming Zhou, Lei Liu

et al.

Journal of Analysis and Testing, Journal Year: 2022, Volume and Issue: 6(1), P. 44 - 52

Published: Feb. 28, 2022

Language: Английский

Citations

61
MnO2 nanosheets as a carrier and accelerator for improved live-cell biosensing application of CRISPR/Cas12a DOI Creative Commons
Dongxia Wang, Yaxin Wang, Jing Wang

et al.

Chemical Science, Journal Year: 2022, Volume and Issue: 13(15), P. 4364 - 4371

Published: Jan. 1, 2022

Besides gene-editing, the CRISPR/Cas12a system has also been widely used in vitro biosensing, but its applications live-cell biosensing are rare. One reason is lacking appropriate carriers to synchronously deliver all components of into living cells. Herein, we demonstrate that MnO2 nanosheets an excellent carrier due two important roles played by them. Through a simple mixing operation, can be loaded on and thus delivered Intracellular glutathione (GSH)-induced decomposition not only results rapid release cells provides Mn2+ as accelerator promote CRISPR/Cas12a-based intracellular targets. Due merits highly efficient delivery, release, accelerated signal output reaction, work better than commercial liposome analysis survivin messenger RNA (mRNA), producing much brighter fluorescence images shorter time. The use might provide good for different CRISPR/Cas systems achieve sensitive targets, paving promising way

Language: Английский

Citations

52
CRISPR-Cas12a based fluorescence assay for organophosphorus pesticides in agricultural products DOI
Ruijie Fu, Yiwen Wang,

Yanlin Liu

et al.

Food Chemistry, Journal Year: 2022, Volume and Issue: 387, P. 132919 - 132919

Published: April 7, 2022

Language: Английский

Citations

46
Quantum Dot-Based Molecular Beacons for Quantitative Detection of Nucleic Acids with CRISPR/Cas(N) Nucleases DOI
Christopher M. Green, Joseph R. Spangler, Kimihiro Susumu

et al.

ACS Nano, Journal Year: 2022, Volume and Issue: 16(12), P. 20693 - 20704

Published: Nov. 15, 2022

Strategies utilizing the CRISPR/Cas nucleases Cas13 and Cas12 have shown great promise in development of highly sensitive rapid diagnostic assays for detection pathogenic nucleic acids. The most common approaches fluorophore-quencher molecular beacons require strand amplification strategies or optical setups to overcome limitations readout. Here, we demonstrate a flexible strategy assembling luminescent colorimetric quantum dot-nucleic acid hairpin (QD-HP) use diagnostics. This utilizes chimeric peptide-peptide (peptide-PNA) conjugate fluorescently labeled DNA RNA hairpins ZnS-coated QDs. QDs are particularly promising alternatives due their greater brightness, strong UV absorbance with large emission offset, exceptional photostability, potential multiplexing sharp peaks. Using Förster resonance energy transfer (FRET), developed ratiometric reporters capable pM target (without nucleotide amplification) both RNA, further demonstrated capabilities camera-phone detection. flexibility this system is imparted by dual functionality QD as FRET donor central nanoscaffold arranging acids fluorescent acceptors on its surface. method also provides generalized approach that could be applied other nuclease systems.

Language: Английский

Citations

44
Sensitive and Amplification-Free Electrochemiluminescence Biosensor for HPV-16 Detection Based on CRISPR/Cas12a and DNA Tetrahedron Nanostructures DOI

Linying Yu,

Yao Peng,

Mengting Sheng

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(7), P. 2852 - 2858

Published: July 4, 2023

Rapid and accurate detection of biomarkers was very important for early screening treatment diseases. Herein, a sensitive amplification-free electrochemiluminescence (ECL) biosensor based on CRISPR/Cas12a DNA tetrahedron nanostructures (TDNs) constructed. Briefly, 3D TDN self-assembled the Au nanoparticle-deposited glassy carbon electrode surface to construct biosensing interface. The presence target would activate trans-cleavage activity Cas12a-crRNA duplex cleave single-stranded signal probe vertex TDN, causing Ru(bpy)32+ fall from weakened ECL signal. Thus, system transduced change concentration into an enabling HPV-16. specific recognition HPV-16 made have good selectivity, while TDN-modified sensing interface could reduce cleaving steric resistance improve performance CRISPR/Cas12a. In addition, pretreated complete sample within 100 min with limit 8.86 fM, indicating that developed possesses potential application prospect fast nucleic acid detection.

Language: Английский

Citations

42
Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges, and Perspectives DOI
Weihong Yin, Jianjian Zhuang, Jiale Li

et al.

Small, Journal Year: 2023, Volume and Issue: 19(49)

Published: Aug. 23, 2023

Abstract Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of in sample with low equipment requirements and simple operations, which be widely used clinical vitro diagnosis. Recently, isothermal amplification technologies such as recombinase polymerase (RPA), loop‐mediated (LAMP), clustered regularly interspaced short palindromic repeats‐CRISPR associated proteins (CRISPR‐Cas) assisted have become a hot spot attention state‐of‐the‐art digital chips provided powerful tool for these technologies. Herein, including RPA, LAMP, CRISPR‐Cas methods, recently, been reviewed. Moreover, challenges possible strategies to address them are discussed. Finally, future directions technology, microfluidic chip device manufacturing, multiplex detection, one‐pot outlined.

Language: Английский

Citations

38
Signal amplification strategies in electrochemiluminescence biosensors DOI
Dong Han, Kui Yang, Shiguo Sun

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 476, P. 146688 - 146688

Published: Oct. 14, 2023

Language: Английский

Citations

32
CRISPR-Cas assisted diagnostics: A broad application biosensing approach DOI Creative Commons
Annalisa Masi, Amina Antonacci, Maria Moccia

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 162, P. 117028 - 117028

Published: March 18, 2023

In addition to its remarkable genome editing capability, the CRISPR-Cas system has proven be very effective in many fields of application, including biosensing pathogenic infections, mutagenic defects, or early cancer diagnosis. Thanks their advantages terms simplicity, efficiency, and reduced time, several systems have been described for design sensitive selective analytical tools, paving way development further commercialization next-generation diagnostics. However, CRISPR-Cas-based biosensors still need research efforts improve some drawbacks, such as target amplification, low reproducibility, lack knowledge exploited element robustness. This review aims describe latest trends technologies better highlight insights point out limitations that overcome future market entry medical

Language: Английский

Citations

30