Journal of Material Science and Technology, Journal Year: 2023, Volume and Issue: 167, P. 50 - 58
Published: June 26, 2023
Language: Английский
Tissue Engineering and Regenerative Medicine, Journal Year: 2021, Volume and Issue: 18(4), P. 499 - 511
Published: July 14, 2021
Abstract Exosomes are cell-secreted nano-sized vesicles which deliver diverse biological molecules for intercellular communication. Due to their therapeutic potential, exosomes have been engineered in numerous ways efficient delivery of active pharmaceutical ingredients various target organs, tissues, and cells. In vivo administered normally delivered the liver, spleen, kidney, lung, gastrointestinal tract show rapid clearance from blood circulation after systemic injection. The biodistribution pharmacokinetics (PK) can be modulated by engineering factors such as cellular origin membrane protein composition exosomes. Recent advances accentuate potential targeted even most challenging organs including central nervous system. Major breakthroughs made related imaging techniques monitoring PK exosomes, well exosomal surface technologies inducing targetability. For delivery, express targeting moieties via direct modification methods chemically modifying surfaces with covalent/non-covalent bonds, or indirect genetically exosome-producing this review, we describe current knowledge determining targetability organotropism monitor addition, highlight recent strategies specific
Language: Английский
Citations
159
Nanomaterials, Journal Year: 2021, Volume and Issue: 11(6), P. 1481 - 1481
Published: June 3, 2021
Exosomes as nanosized vesicles are emerging drug delivery systems for therapeutics owing to their natural origin, ability mediate intercellular communication, and potential encapsulate various biological molecules such proteins nucleic acids within the lipid bilayer membrane or in lumen. contain endogenous components (proteins, lipids, RNA) that could be used deliver cargoes target cells, offering an opportunity diagnose treat diseases. Owing travel safely extracellular fluid transport cells with high efficacy, exosomes offer enhanced of vivo. However, several challenges related stabilization exosomes, production sufficient amounts safety efficient loading drugs into clearance from circulation, transition bench scale clinical may limit development use. For use it is important understand molecular mechanisms behind function exosome vesicles. This review exploits techniques isolation characterization enhance therapeutic outcome methods. Further, routes administration, trials, regulatory aspects will discussed this review.
Language: Английский
Citations
144
ACS Nano, Journal Year: 2022, Volume and Issue: 16(1), P. 869 - 884
Published: Jan. 5, 2022
The treatment of Parkinson's disease (PD) has been hindered by the complex pathologies and multiple membrane barriers during drug delivery. Although exosomes derived from mesenchymal stem cells (MSCs) have great potential for PD, MSC-derived alone could not fully meet therapeutic requirements due to their limitation in therapy Here, we develop a self-oriented nanocarrier called PR-EXO/PP@Cur that combines with curcumin. can be across directly release drugs into cytoplasm target after intranasal administration. With enhanced accumulation action site, achieves three-pronged synergistic deal PD reducing α-synuclein aggregates, promoting neuron function recovery, alleviating neuroinflammation. After PR-EXO/PP@Cur, movement coordination ability model mice are significantly improved. These results show prospects or other neurodegenerative diseases.
Language: Английский
Citations
128
Biomarker Research, Journal Year: 2022, Volume and Issue: 10(1)
Published: May 12, 2022
Abstract Exosomes, ranging in size from 30 to 150 nm as identified initially via electron microscopy 1946, are one of the extracellular vesicles (EVs) produced by many cells and have been subject studies; initially, they were considered cell wastes with belief that exosomes maintain homeostasis. Nowadays, it has found EVs secreted different play a vital role cellular communication usually both physiological pathological conditions. Due presence markers ligands on surface exosomes, paracrine, endocrine autocrine effects some cases. Immune cells, like other can secrete interact surrounding these vesicles. system cells-derived (IEXs) induce responses, such increasing decreasing transcription various genes regulating cytokine production. This review deliberate function innate acquired immune derived their pathogenesis diseases, therapeutic appliances.
Language: Английский
Citations
98
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(13)
Published: Feb. 5, 2023
Abstract Myocardial infarction (MI) is a cardiovascular disease that poses serious threat to human health. Uncontrolled and excessive cardiac fibrosis after MI has been recognized as primary contributor mortality by heart failure. Thus, prevention of or alleviation progression important for repair. To this end, biocompatible microneedle (MN) patch based on gelatin fabricated load exosomes containing microRNA‐29b (miR‐29b) mimics with antifibrotic activity prevent MI. Exosomes are isolated from umbilical cord mesenchymal stem cells loaded miR‐29b via electroporation, which can be internalized effectively in fibroblasts upregulate the expression downregulate fibrosis‐related proteins. After being implanted infarcted mouse model, MN increase retention myocardium, leading inflammation, reduction infarct size, inhibition fibrosis, improvement function. This design explored suitable platform deliver biomolecules locally showing potential treatment clinical applications.
Language: Английский
Citations
84
RSC Advances, Journal Year: 2022, Volume and Issue: 13(1), P. 80 - 114
Published: Dec. 20, 2022
In the field of targeted drug delivery, effects size and morphology nanocarriers are great importance need to be discussed in depth. To concise, among all various shapes nanocarriers, rods tubes with a narrow cross-section most preferred for penetration cell membrane. this regard, several studies have focused on methods produce nanorods nanotubes controlled optimized aspect ratio (AR). Additionally, non-spherical orientation could affect cellular uptake process while tangent angle less than 45° is better at penetrating membrane,
Language: Английский
Citations
83
Journal of Controlled Release, Journal Year: 2022, Volume and Issue: 348, P. 723 - 744
Published: June 23, 2022
Language: Английский
Citations
82
Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)
Published: Sept. 16, 2023
Abstract Extracellular vesicles (EVs) are 30–150 nm membrane-bound naturally secreted by cells and play important roles in intercellular communication delivering regulatory molecules such as proteins, lipids, nucleic acids metabolites to recipient cells. As natural nano-carriers, EVs possess desirable properties high biocompatibility, biological barrier permeability, low toxicity, immunogenicity, making them potential therapeutic delivery vehicles. derived from specific have inherent targeting capacity towards cell types, which is yet not satisfactory enough for targeted therapy development needs be improved. Surface modifications endow with abilities, significantly improving their efficiency. Herein, we first briefly introduce the biogenesis, composition, uptake function of EVs, review cargo loading approaches EVs. Then, summarize recent advances surface engineering strategies focusing on applications engineered therapy. Altogether, hold great promise various cargos, show promising effects multiple diseases. Graphical
Language: Английский
Citations
56
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 7923 - 7940
Published: Dec. 1, 2023
Exosomes are nano-sized membrane vesicles that transfer bioactive molecules between cells and modulate various biological processes under physiological pathological conditions. By applying bioengineering technologies, exosomes can be modified to express specific markers or carry therapeutic cargo emerge as novel platforms for the treatment of cancer, neurological, cardiovascular, immune, infectious diseases. However, there many challenges uncertainties in clinical translation exosomes. This review aims provide an overview recent advances engineered exosomes, with a special focus on methods strategies loading drugs into pros cons different methods, optimization exosome production based encapsulated. Moreover, we also summarize current applications prospects well potential risks limitations need addressed engineering, including standardization preparation engineering protocols, quality quantity control drug release, immunogenicity cytotoxicity Overall, represent exciting frontier nanomedicine, but they still face large-scale production, maintenance storage stability, translation. With continuous this field, exosome-based formulation could offer great promise targeted human
Language: Английский
Citations
56
Extracellular Vesicle, Journal Year: 2023, Volume and Issue: 3, P. 100032 - 100032
Published: Dec. 9, 2023
Exosomes are nanovesicles released from cells due to pathophysiological events. These nanoparticles resistant metabolic destruction and can transcend the blood-brain barrier. Exosome therapy could be employed as precision medicine by targeting underlying etiology. This article briefly elucidates basic physiology of exosomes, their types, characteristics, cargo that for drug administration. It then delves into therapeutic applications, such oncology, neurological disorders, regenerative medicine. Exosome-based delivery using small molecules, nucleic acids, proteins is also demonstrated. Finally, global organizations successfully creating testing medicinal biomaterials highlighted.
Language: Английский
Citations
50