A Self-Immolative DNA Nanogel Vaccine toward Cancer Immunotherapy DOI
Yuwei Hu, Shujun Gao, Hongfang Lu

et al.

Nano Letters, Journal Year: 2023, Volume and Issue: 23(21), P. 9778 - 9787

Published: Oct. 25, 2023

The development of precisely engineered vehicles for intracellular delivery and the controlled release payloads remains a challenge. DNA-based nanomaterials offer promising solution based on A-T-G-C alphabet-dictated predictable assembly high programmability. Herein, we present self-immolative DNA nanogel vaccine, which can be tracelessly released in compartments activate immune response. Three building blocks with cytosine-rich overhang domains are designed to self-assemble into framework size. Two oligo agonists one antigen peptide conjugated via an acid-labile chemical linker. Upon internalization acidic endosomes, formation i-motif configurations leads dissociation vaccine. linker is cleaved, releasing their traceless original form antigen-presenting cells This study presents novel strategy constructing platforms intracellularly stimuli-triggered therapeutics.

Language: Английский

A Cell Selective Fluoride-Activated MOF Biomimetic Platform for Prodrug Synthesis and Enhanced Synergistic Cancer Therapy DOI
Bin Zhang, Chun Liu, Zhengwei Liu

et al.

ACS Nano, Journal Year: 2022, Volume and Issue: 16(12), P. 20975 - 20984

Published: Nov. 17, 2022

As a burgeoning bioorthogonal reaction, the fluoride-mediated desilylation is capable of prodrug activation. However, due to reactions lack cell selectivity and unitary therapy modality, this strongly impedes their biomedical applications. Herein, we construct cancer cell-selective biomimetic metal–organic framework (MOF)-F platform for activation enhanced synergistic chemodynamic (CDT). With membranes camouflage, designed nanocatalyst displays preferential accumulation homotypic cells. Then, pH-responsive releases fluoride ions ferric ions. For our tert-butyldimethyl silyl (TBS)-hydroxycamptothecin (TBSO-CPT), can desilylate TBS cleave ether linker synthesize OH-CPT (10-hydroxycamptothecin) drug molecule, which effectively kills Intriguingly, bioorthogonal-synthesized upregulates intracellular H2O2 by activating nicotinamide adenine dinucleotide phosphate oxidase (NOX), amplifying released iron induced Fenton reaction CDT. Both in vitro vivo studies demonstrate strategy presents versatile fluoride-activated catalyst synthesis. Our work may accelerate applications chemistry.

Language: Английский

Citations

33
Spatiotemporal Ultrasound‐Driven Bioorthogonal Catalytic Therapy DOI
Lili Xia, Meng Chen, Caihong Dong

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 35(7)

Published: Nov. 22, 2022

Abstract Bioorthogonal chemistry, referring to the rapid and selective synthesis of imaging and/or therapeutic molecules in live animals via transition metal‐mediated non‐natural chemical transformation without disrupting endogenous reactions, has greatly expanded tools techniques for biomedicine. However, owing safety concerns associated with metal toxicity, selectivity, sensitivity stability, efficient bioorthogonal reactions that can be reliably executed complex biological environments remain challenging. In this study, an intelligent, versatile catalyst based on ultrasmall poly(acrylic acid)‐modified copper nanocomplexes (Cu@PAA NCs) achieve high spatiotemporal catalytic efficacy is established. The activity Cu@PAA NCs reversibly regulated valence state interconversion between Cu(II) Cu(I) under exogenous ultrasound irradiation, promoting off‐target prodrug activation lesion sites through Cu(I)‐catalyzed azide–alkyne cycloaddition reaction. Moreover, ultrasound‐triggered electron–hole separation endows robust sonosensitizing ability sonodynamic therapy. Furthermore, exhibit enhanced contrast magnetic resonance photoacoustic imaging. Notably, renal‐clearable intrinsically benign biocompatibility. This spatiotemporally ultrasound‐mediated catalysis not only expands repertoire situ agents but also provides a new avenue disease theranostics.

Language: Английский

Citations

32
Metal-free polymer nano-photosensitizer actuates ferroptosis in starved cancer DOI Creative Commons
Dapeng Chen, Liang Chen, Xinyu Qu

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 292, P. 121944 - 121944

Published: Dec. 6, 2022

Language: Английский

Citations

30
The 2022 Nobel Prize in Chemistry for the development of click chemistry and bioorthogonal chemistry DOI Open Access
Joseph Zaia

Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(4), P. 527 - 532

Published: Jan. 5, 2023

Language: Английский

Citations

21
A Self-Immolative DNA Nanogel Vaccine toward Cancer Immunotherapy DOI
Yuwei Hu, Shujun Gao, Hongfang Lu

et al.

Nano Letters, Journal Year: 2023, Volume and Issue: 23(21), P. 9778 - 9787

Published: Oct. 25, 2023

The development of precisely engineered vehicles for intracellular delivery and the controlled release payloads remains a challenge. DNA-based nanomaterials offer promising solution based on A-T-G-C alphabet-dictated predictable assembly high programmability. Herein, we present self-immolative DNA nanogel vaccine, which can be tracelessly released in compartments activate immune response. Three building blocks with cytosine-rich overhang domains are designed to self-assemble into framework size. Two oligo agonists one antigen peptide conjugated via an acid-labile chemical linker. Upon internalization acidic endosomes, formation i-motif configurations leads dissociation vaccine. linker is cleaved, releasing their traceless original form antigen-presenting cells This study presents novel strategy constructing platforms intracellularly stimuli-triggered therapeutics.

Language: Английский

Citations

20