Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.
Language: Английский
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)
Published: Jan. 18, 2024
Abstract Dielectrophoresis (DEP) particle separation has label‐free, well‐controllable, and low‐damage merits. Sidewall microelectrodes made of liquid metal alloy (LMA) inherits the additional advantage thick electrodes to generate impactful DEP force. However, existing LMA electrode‐based devices lack ability integrate large‐array in a compact footprint, severely limiting flow rate thus throughput. Herein, facile versatile method is proposed high‐density microfluidic devices, taking passive control capillary burst valves (CBVs). CBVs with carefully designed pressures are co‐designed channels, allowing self‐assembly electrode array through simple hand‐push injection. The arrayed configuration brings accumulative deflection effect. Specifically, fabricated 5000 pairs sidewall chip demonstrted achieve ten times higher throughput deflection. 5000‐electrode‐pair device applied successfully separate four mixed samples, including human peripheral blood mononuclear cells A549 70 µL min −1 . It envisioned that this work can greatly facilitate fabrication offer robust platform for applications.
Language: Английский
Citations
33
Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(24), P. 4792 - 4804
Published: Jan. 1, 2022
We present the current status, challenges, and future opportunities of inertial microfluidics.
Language: Английский
Citations
68
Lab on a Chip, Journal Year: 2022, Volume and Issue: 23(5), P. 982 - 1010
Published: Nov. 1, 2022
Manipulation and separation of submicron nanoparticles are indispensable in many chemical, biological, medical, environmental applications. Conventional technologies such as ultracentrifugation, ultrafiltration, size exclusion chromatography, precipitation immunoaffinity capture limited by high cost, low resolution, purity or the risk damage to biological particles. Microfluidics can accurately control fluid flow channels with dimensions tens micrometres. Rapid microfluidics advancement has enabled precise sorting isolating better resolution efficiency than conventional technologies. This paper comprehensively studies latest progress microfluidic technology for nanoparticle manipulation. We first summarise principles traditional techniques manipulating nanoparticles. Following classification active, passive, hybrid approaches, we elaborate on physics, device design, working mechanism applications each technique. also compare merits demerits different benchmark them Concurrently, seven standard post-separation detection Finally, discuss current challenges future perspectives manipulation separation.
Language: Английский
Citations
60
Small, Journal Year: 2023, Volume and Issue: 19(15)
Published: Jan. 11, 2023
Abstract Liquid biopsy for the analysis of circulating cancer biomarkers (CBs) is a major advancement toward early detection cancer. In comparison to tissue techniques, liquid relatively painless, offering multiple sampling opportunities across easily accessible bodily fluids such as blood, urine, and saliva. also inexpensive simple, avoiding requirement specialized laboratory equipment or trained medical staff. Major advances in field are attributed largely developments nanotechnology microfabrication that enables creation highly precise chip‐based platforms. These devices can overcome limitations an individual biomarker by detecting markers simultaneously on same chip, featuring integrated combined target separation techniques. this review, portable semi‐portable micro, nano, multiplexed platforms CB diagnosis highlighted. A comparative discussion provided, noting merits drawbacks platforms, especially terms portability. Finally, key challenges device portability possible solutions, well discussing future direction
Language: Английский
Citations
41
Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(15), P. 2789 - 2800
Published: Jan. 1, 2022
Inertial microfluidics functions solely based on the fluid dynamics at relatively high flow speed. Thus, channel geometry is critical design parameter that contributes to performance of device. Four basic geometries (i.e., straight, expansion-contraction, spiral and serpentine) have been proposed extensively studied. To further enhance performance, innovative through combining two or more promising. This work explores embedding periodic concave convex obstacle microstructures in sinusoidal channels investigates their influence particle inertial focusing separation. The obstacles could significantly Dean tune range for Based this finding, we propose a cascaded device by connecting consecutively rare cell are embedded second adapt its operational rates enable functional operation both channels. Polystyrene beads breast cancer cells (T47D) spiking blood were respectively processed results indicate an outstanding separation with 3 4 orders magnitude enhancement purity samples primary ratio 0.01% 0.001%, respectively. Embedding as brings flexibility microfluidic devices, offering feasible new way combine serial processing units high-performance
Language: Английский
Citations
39
Electrophoresis, Journal Year: 2023, Volume and Issue: 44(11-12), P. 910 - 937
Published: April 16, 2023
Abstract Microfluidic separation of particles and cells is crucial to lab‐on‐a‐chip applications in the fields science, engineering, industry. The continuous‐flow methods can be classified as active or passive depending on whether force involved process externally imposed internally induced. majority current separations have been realized using only one methods. Such a single‐mode usually limited one‐parameter separation, which often becomes less effective even ineffective when dealing with real samples because their inherent heterogeneity. Integrating two more either type has demonstrated offer several advantages like improved specificity, resolution, throughput. This article reviews recent advances such multimode particle cell microfluidic devices, including serial‐mode prefocused multistage parallel‐mode force‐tuned separation.
Language: Английский
Citations
35
Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 1982 - 2035
Published: Jan. 1, 2024
Many human tissues exhibit a highly oriented architecture that confers them with distinct mechanical properties, enabling adaptation to diverse and challenging environments. Hydrogels, their water-rich "soft wet" structure, have emerged as promising biomimetic materials in tissue engineering for repairing replacing damaged organs. Highly hydrogels can especially emulate the structural orientation found tissue, exhibiting unique physiological functions properties absent traditional homogeneous isotropic hydrogels. The design preparation of involve strategies like including nanofillers, polymer-chain networks, void channels, microfabricated structures. Understanding specific mechanism action how these affect cell behavior biological applications such cornea, skin, skeletal muscle, tendon, ligament, cartilage, bone, blood vessels, heart, etc., requires further exploration generalization. Therefore, this review aims fill gap by focusing on strategy application field engineering. Furthermore, we provide detailed discussion various organs mechanisms through which structures influence behavior.
Language: Английский
Citations
14
Microsystems & Nanoengineering, Journal Year: 2024, Volume and Issue: 10(1)
Published: March 12, 2024
Abstract Tumor cell clusters are regarded as critical factors in cancer pathophysiology, and increasing evidence of their higher treatment resistance metastasis compared to single tumor cells has been obtained. However, existing separation methods that designed for cannot be used simultaneously purify clusters. To address this problem, we demonstrated a microfluidic approach the high-throughput, continuous-flow ternary cells, clusters, WBCs from clinical pleural or abdominal effusions by coupling slanted spiral channels periodic contraction-expansion arrays. We first systematically explored influence particle size flow rate on focusing. The performance indicated 94.0% were removed more than 97% MDA-MB-231 recovered at high 3500 µL/min. Moreover, 90% effectively preserved after separation. Finally, successfully applied our device different malignant collected patients with metastatic cancer. Thus, spiral-contraction-expansion potential sample pretreatment tool cytological diagnosis effusions.
Language: Английский
Citations
13
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 491, P. 152067 - 152067
Published: May 9, 2024
Language: Английский
Citations
10
Sensors, Journal Year: 2025, Volume and Issue: 25(5), P. 1577 - 1577
Published: March 4, 2025
Surface acoustic wave (SAW)-based microfluidics has emerged as a promising technology for precisely manipulating particles and cells at the micro- nanoscales. Acoustofluidic devices offer advantages such low energy consumption, high throughput, label-free operation, making them suitable particle manipulation tasks including pumping, mixing, sorting, separation. In this review, we provide an overview discussion of recent advancements in SAW-based microfluidic nanoparticle manipulation. Through thorough investigation literature, explore interdigitated transducer designs, materials, fabrication techniques, channel properties, SAW operational modes acoustofluidic devices. actuators are mainly based on lithium niobate piezoelectric transducers, with plethora wavelengths, dimensions, configurations, applied different fluid methods: We observed accuracy sorting across size ranges discussed alternative device configurations to enhance sensitivity. Additionally, collected data show successful implementation real-world applications medical diagnostics environmental monitoring. By critically analyzing approaches, identified common trends, challenges, potential areas improvement microfluidics. Furthermore, discuss current state-of-the-art opportunities further research development field.
Language: Английский
Citations
1