Efficient Extraction of White Blood Cells Using Modular Inertial Microfluidics DOI

Mingqi Yang,

Chen Ni,

Dan Wu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

The existence of massive amounts red blood cells (RBCs) and other cell components poses a challenge for the efficient extraction white (WBCs) from blood. Herein, we proposed modular inertial microfluidics WBCs lysed samples. different WBC needs could be met by flexibly combining washing module concentration in microfluidics. First, respective optimal flow rates modules with channel sizes were explored. Then, based on combination modules, three modes to meet processing large-volume sample, low volume expansion, easy operation. replacement efficiency recovery rate target particles all greater than 93%. Finally, samples was tested. combined use Modes 1 2 achieved 93.1% 93.6%, while single Mode 3 97.1% 95.9%. Compared traditional centrifuges, our showed better performance reduced residual RBC fragments. More importantly, it provided an excellent free capability needs.

Language: Английский

Recent advances in multimode microfluidic separation of particles and cells DOI Creative Commons
Yongxin Song, Deyu Li, Xiangchun Xuan

et al.

Electrophoresis, Journal Year: 2023, Volume and Issue: 44(11-12), P. 910 - 937

Published: April 16, 2023

Abstract Microfluidic separation of particles and cells is crucial to lab‐on‐a‐chip applications in the fields science, engineering, industry. The continuous‐flow methods can be classified as active or passive depending on whether force involved process externally imposed internally induced. majority current separations have been realized using only one methods. Such a single‐mode usually limited one‐parameter separation, which often becomes less effective even ineffective when dealing with real samples because their inherent heterogeneity. Integrating two more either type has demonstrated offer several advantages like improved specificity, resolution, throughput. This article reviews recent advances such multimode particle cell microfluidic devices, including serial‐mode prefocused multistage parallel‐mode force‐tuned separation.

Language: Английский

Citations

35
Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review DOI Creative Commons
A. Farahinia, Wenjun Zhang, Ildikó Badea

et al.

Sensors, Journal Year: 2023, Volume and Issue: 23(11), P. 5300 - 5300

Published: June 2, 2023

The treatment of cancers is a significant challenge in the healthcare context today. Spreading circulating tumor cells (CTCs) throughout body will eventually lead to cancer metastasis and produce new tumors near healthy tissues. Therefore, separating these invading extracting cues from them extremely important for determining rate progression inside development individualized treatments, especially at beginning process. continuous fast separation CTCs has recently been achieved using numerous techniques, some which involve multiple high-level operational protocols. Although simple blood test can detect presence circulation system, detection still restricted due scarcity heterogeneity CTCs. more reliable effective techniques thus highly desired. technology microfluidic devices promising among many other bio-chemical bio-physical technologies. This paper reviews recent developments two types devices, are based on size and/or density cells, cells. goal this review identify knowledge or gaps suggest future works.

Language: Английский

Citations

27
Microfluidic systems for particle capture and release: A review DOI Creative Commons
Liyuan Gong,

Andrew Cretella,

Yang Lin

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 236, P. 115426 - 115426

Published: May 25, 2023

Language: Английский

Citations

24
Cascaded elasto-inertial separation of malignant tumor cells from untreated malignant pleural and peritoneal effusions DOI Creative Commons

Chen Ni,

Dan Wu, Chen Yao

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(4), P. 697 - 706

Published: Jan. 1, 2024

A cascaded elasto-inertial cell separation device for pretreatment-free, high-recovery-ratio, and high-purity of malignant tumor cells from clinical pleural peritoneal effusions.

Language: Английский

Citations

14
Applications of dielectrophoresis in microfluidic-based exosome separation and detection DOI
Lan Mei, Fang Yang

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 491, P. 152067 - 152067

Published: May 9, 2024

Language: Английский

Citations

10
A spiral channel with integrated microelectrodes for label-free particle lateral position and size characterization DOI
Yunhao Peng, Bruce K. Gale, Himanshu J. Sant

et al.

Biomedical Microdevices, Journal Year: 2025, Volume and Issue: 27(2)

Published: March 18, 2025

Language: Английский

Citations

1
Insights into microfabrication and implementation of microfluidics in pharmaceutical drug delivery and analysis DOI Creative Commons

Omnia A. Al-wdan,

Omnya A. Sharallah,

Nada A. Abdelwahab

et al.

OpenNano, Journal Year: 2023, Volume and Issue: 12, P. 100156 - 100156

Published: May 1, 2023

Microfluidics are systems that set forth valuable means to efficiently control fluid movement and dynamics in specially fabricated micro-channel systems. The advancement of the unique design microfluidics technology can offer an alternative traditional used many pharmaceutical applications. In this context, review presents a vivid depiction overview various mixing techniques mechanisms. Insights on how microfluidic methodologies be utilized efficient manufacture drug delivery e.g., liposomes, microparticles, nanoparticles, nano-in-microparticles provided. Moreover, different analytical applications such as lab-on-chip, dielectrophoresis, droplet microfluidic, digital addressed. Specifically, approaches for antibody screening Cell-free protein synthesis system highlighted. Lastly, modular approach planning constructing is explored key player implementing futuristic custom-made Collectively, hold potential domains including biosensors, point-of-care diagnostics, health monitoring patient care management.

Language: Английский

Citations

20
Size-Tunable Elasto-Inertial Sorting of Haematococcus pluvialis in the Ultrastretchable Microchannel DOI
Zixuan Jia, Jialin Wu,

Xiuru Wu

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(35), P. 13338 - 13345

Published: Aug. 16, 2023

Haematococcus pluvialis is a good source of astaxanthin, which reduces oxidation in the human body, treats inflammation, and slows growth breast skin cancer cells. Since size H. often closely related to astaxanthin yield, size-based microalgal separation has far-reaching significance for high-value algae extraction algal directed evolution. In this work, we report novel size-tunable elasto-inertial sorting Ecoflex ultrastretchable microfluidic devices. chips can deform be flexible, bringing flexibility stretchability microchannels as well new possibilities large-scale modulation channel geometry. Here, effects velocity, elongation, particle on migration particles are systematically studied. We found that elongation strong regulating effect focusing. addition, verified continuous regulation threshold cells by stretching channel, providing technical support evolution high-yield microalgae.

Language: Английский

Citations

20
A review on inertial microfluidic fabrication methods DOI Creative Commons
Zohreh Akbari, Mohammad Amin Raoufi, Sheyda Mirjalali

et al.

Biomicrofluidics, Journal Year: 2023, Volume and Issue: 17(5)

Published: Sept. 1, 2023

In recent decades, there has been significant interest in inertial microfluidics due to its high throughput, ease of fabrication, and no need for external forces. The focusing efficiency microfluidic systems relies entirely on the geometrical features microchannels because hydrodynamic forces (inertial lift Dean drag forces) are main driving devices. past few years, novel microchannel structures have propounded improve particle manipulation efficiency. However, fabrication these unconventional remained a serious challenge. Although researchers pushed forward frontiers microfabrication technologies, techniques employed not discussed comprehensively. This review introduces approaches used creating microchannels, including photolithography, xurography, laser cutting, micromachining, microwire technique, etching, hot embossing, 3D printing, injection molding. advantages disadvantages methods also discussed. Then, reviewed regarding resolution, structures, cost, materials. provides thorough insight into manufacturing which could be helpful future studies harvesting yield resolution by choosing proper technique.

Language: Английский

Citations

18
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel DOI Creative Commons
Md. Sadiqul Islam, Xiaolin Chen

Biotechnology Progress, Journal Year: 2023, Volume and Issue: 39(4)

Published: March 27, 2023

The efficient isolation of viable and intact circulating tumor cells (CTCs) from blood is critical for the genetic analysis cancer cells, prediction progression, development drugs, evaluation therapeutic treatments. While conventional cell separation devices utilize size difference between CTCs other they fail to separate white (WBCs) due significant overlap. To overcome this issue, we present a novel approach that combines curved contraction-expansion (CE) channels with dielectrophoresis (DEP) inertial microfluidics isolate WBCs regardless This label-free continuous method utilizes dielectric properties variation WBCs. results demonstrate proposed hybrid microfluidic channel can effectively A549 their throughput 300 μL/min, achieving high distance 233.4 μm at an applied voltage 50 Vp-p . allows modification migration characteristics by controlling number CE sections channel, voltage, frequency, flow rate. With its unique features single-stage separation, simple design, tunability, provides promising alternative existing techniques may have wide range applications in biomedicine.

Language: Английский

Citations

17