Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 26, 2025
Drug-induced
liver
injury
(DILI)
has
emerged
as
an
urgent
clinical
challenge.
It
is
characterized
by
mitochondrial
dysfunction
in
cells,
which
leads
to
abnormal
changes
H2O2
levels
within
the
mitochondria.
Super-resolution
imaging
allows
for
observation
of
fine
structure
mitochondria
at
nanometer
scale,
potentially
enabling
detection
during
DILI
subcellular
organelle
level.
Here,
we
report
design
and
synthesis
a
novel
H2O2-activated
probe
levels.
SML
near-infrared
(NIR)
fluorescent
with
large
Stokes
shift
(260
nm)
sensing
mechanism
based
on
intramolecular
charge
transfer
(ICT)
switching.
was
conducted
using
structured
illumination
microscopy
(SIM).
The
improved
accuracy
observing
periods
be
effectively
utilized
rapid
monitoring
nanoscale
upregulation
hepatic
fibrosis,
thus
providing
capability
screen
effective
therapeutic
candidates.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(7), P. 4124 - 4257
Published: March 21, 2024
Hydrogen
sulfide
(H2S)
is
not
only
a
well-established
toxic
gas
but
also
an
important
small
molecule
bioregulator
in
all
kingdoms
of
life.
In
contemporary
biology,
H2S
often
classified
as
"gasotransmitter,"
meaning
that
it
endogenously
produced
membrane
permeable
carries
out
essential
cellular
processes.
Fluorescent
probes
for
and
related
reactive
sulfur
species
(RSS)
detection
provide
cornerstone
investigating
the
multifaceted
roles
these
molecules
complex
biological
systems.
A
now
common
approach
to
develop
such
tools
"activity-based
probes"
couple
specific
H2S-mediated
chemical
reaction
fluorescent
output.
This
Review
covers
different
types
highlights
mechanisms
by
which
each
probe
type
activated
RSS.
Common
examples
include
reduction
oxidized
nitrogen
motifs,
disulfide
exchange,
electrophilic
reactions,
metal
precipitation,
coordination.
addition,
we
outline
complementary
activity-based
imaging
reductant-labile
sulfane
species,
including
persulfides
polysulfides.
For
highlighted
this
Review,
focus
on
systems
with
demonstrated
compatibility
or
applications.
Building
from
breadth
reported
strategies
application,
highlight
key
unmet
challenges
future
opportunities
advancing
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(15), P. 5992 - 6000
Published: April 4, 2024
Hypochlorous
acid
(HClO)
is
a
typical
endogenous
ROS
produced
mainly
in
mitochondria,
and
it
has
strong
oxidative
properties.
Abnormal
HClO
levels
lead
to
mitochondrial
dysfunction,
strongly
associated
with
various
diseases.
It
been
shown
that
shows
traces
of
overexpression
cells
both
ferroptosis
hepatocellular
carcinoma
(HCC).
Therefore,
visualization
during
HCC
important
explore
its
physiological
pathological
roles.
So
far,
there
no
report
on
the
HCC.
Thus,
we
present
ratiometric
near-infrared
(NIR)
fluorescent
probe
Mito-Rh-S
which
visualized
for
first
time
fluctuation
mitochondria
an
ultrafast
response
rate
(2
s)
large
emission
shift
(115
nm).
was
constructed
based
PET
sensing
mechanism
thus
high
signal-to-noise
ratio.
The
cell
experiments
demonstrated
Fe2+-
erastin-induced
HepG2
resulted
elevated
concentration
Fe2+
erastin
cause
severe
damage
stress
had
potential
kill
cells.
By
regulating
concentration,
induction
time,
treatment
model,
can
accurately
detect
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(13)
Published: Jan. 19, 2024
Abstract
The
design
and
development
of
ultra‐accurate
probe
is
great
significance
to
chemical
sensing
in
complex
practical
scenarios.
Here,
a
self‐accelerating
naphthalimide‐based
with
fast
response
high
sensitivity
toward
hydrogen
peroxide
(H
2
O
)
designed.
By
coupling
the
specially
selected
upconversion
nanoparticles
(UCNPs),
an
colorimetric‐fluorescent‐upconversion
luminescence
(UCL)
tri‐mode
platform
constructed.
Owing
promoted
reaction
process,
this
demonstrates
rapid
(<
1
s),
ultra‐low
detection
limit
(4.34
nM),
superb
anti‐interferent
ability
even
presence
>
21
types
oxidants,
explosives,
metallic
salts,
daily
compounds,
colorful
or
fluorescent
substances.
In
addition,
effectiveness
further
verified
by
sponge‐based
chip
loaded
UCNPs/probe
recognizing
trace
H
vapor
from
interferents
three
characteristic
colors
existing
simultaneously.
proposed
visualization
expected
open
up
brand‐new
methodology
for
sensing.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(33), P. 22055 - 22070
Published: Aug. 8, 2024
The
selective
elimination
of
cytotoxic
ROS
while
retaining
essential
ones
is
pivotal
in
the
management
chronic
inflammation.
Co-occurring
bacterial
infection
further
complicates
conditions,
necessitating
precision
and
an
efficacious
treatment
strategy.
Herein,
dynamic
nanomodulators
are
rationally
constructed
through
regulating
surface
states
herbal
carbon
dots
(CDs)
for
on-demand
inflammation
or
elimination.
phenolic
OH
containing
CDs
derived
from
honeysuckle
(HOCD)
dandelion
(DACD)
demonstrated
appropriate
redox
potentials,
ensuring
their
ability
to
scavenge
such
as
·OH
ONOO–,
invalidity
toward
O2·–,
H2O2,
NO.
This
enables
efficient
without
affecting
signal
pathways.
C–N/C═N
taxus
leaves
(TACD)
DACD
renders
them
with
suitable
band
structures,
facilitating
absorption
red
region
generation
O2·–
upon
light
irradiation
sterilization.
Specifically,
facilely
prepared
demonstrates
fascinating
modulating
ability,
making
it
highly
addressing
concurrent
infection,
diabetic
wound
infection.
regulation
strategy
facilitates
realization
precise
minimal
side
effects,
holding
immense
potential
clinical
practice.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 17, 2025
The
pathogenesis
of
acute
kidney
injury
(AKI)
is
a
multifaceted
process
involving
various
mechanisms,
with
oxidative
stress
playing
crucial
role
in
its
development.
Hypochlorite
(HOCl)
and
cysteine
(Cys)
are
indicators
AKI
pathophysiology,
directly
reflecting
the
degree
disease
severity.
However,
their
exact
mechanism
action
pathophysiology
remains
unknown.
Herein,
we
developed
dual-channel
fluorescent
probe,
MB-NAP,
which
allowed
for
simultaneous
detection
HOCl
Cys.
probe
exhibited
distinct
fluorescence
responses
green
channel
(λex
=
450
nm,
λem
560
nm)
red
610
690
nm),
without
any
spectral
crosstalk,
allowing
accurate
measurement
both
analytes.
We
successfully
applied
MB-NAP
to
monitor
levels
Cys
cellular
vivo
models
AKI,
revealing
significant
increase
concentrations
compared
normal
models.
Furthermore,
was
demonstrated
exhibit
outstanding
capabilities
drug
screening
by
effectively
real-time
monitoring
This
study
not
only
provides
more
sensitive
reliable
method/tool
tracking
AKI-related
pathological
processes
but
also
offers
potential
breakthrough
early
diagnosis
identification
therapeutic
agents
aimed
at
mitigating
stress-induced
damage
AKI.
Smart Molecules,
Journal Year:
2024,
Volume and Issue:
2(3)
Published: Aug. 23, 2024
Abstract
In
situ
precise
detection
of
bioactive
molecules
with
high
sensitivity
and
spatiotemporal
resolution
is
essential
for
studying
physiological
events
disease
diagnosis.
The
utilization
versatile
fluorescent
probes
in
fluorescence
imaging
offers
a
powerful
tool
vivo
biomarkers
closely
associated
pathological
conditions.
However,
the
dynamic
behavior
leading
to
rapid
clearance
small
molecule
from
regions
interest
severely
compromises
their
potential
imaging.
Notably,
self‐immobilizing
that
selectively
recognize
diseased
tissues
while
improving
retention
enrichment
enable
accurate
high‐fidelity
this
review,
we
aim
summarize
strategies
employed
recent
advances
performance
precision
using
techniques.
Lastly,
discuss
prospects
challenges
promote
further
development
application
more
delicate
probes.
