Near-Infrared Probes Designed with Hemicyanine Fluorophores Featuring Rhodamine and 1,8-Naphthalic Derivatives for Viscosity and HSA Detection in Live Cells

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

This paper presents the development of near-infrared (NIR) fluorescent probes,

Language: Английский

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Syntheses and Applications of Coumarin-Derived Fluorescent Probes for Real-Time Monitoring of NAD(P)H Dynamics in Living Cells across Diverse Chemical Environments

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 5437 - 5451

Published: July 12, 2024

Fluorescent probes play a crucial role in elucidating cellular processes, with NAD(P)H sensing being pivotal understanding metabolism and redox biology. Here, the development characterization of three fluorescent probes,

Language: Английский

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Roadmap for Designing Donor-π-Acceptor Fluorophores in UV-Vis and NIR Regions: Synthesis, Optical Properties and Applications

Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 119 - 119

Published: Jan. 14, 2025

Donor acceptor (D-π-A) fluorophores containing a donor unit and an moiety at each end connected by conjugated linker gained attention in the last decade due to their system ease of tunability. These features make them good candidates for various applications such as bioimaging, photovoltaic devices nonlinear optical materials. Upon excitation D-π-A fluorophore, intramolecular charge transfer (ICT) occurs, it polarizes molecule resulting ‘push–pull’ system. The emission wavelengths can be altered from UV-vis NIR region modifying unit, π between them. emitting with restricted molecular rotations are used aggregation-induced (AIE). carboxylic acid cyano groups preferred applications, large surface area two photon absorbing applications. Herein, we report synthesis, properties, region.

Language: Английский

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An activated near-infrared mitochondrion-targetable fluorescent probe for rapid detection of NADH

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(46), P. 5932 - 5935

Published: Jan. 1, 2024

A novel NIR fluorescent probe based on quinoline-conjugated benzo[

Language: Английский

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Dynamic insights into mitochondrial function: Monitoring viscosity and SO2 levels in living cells

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 258, P. 112986 - 112986

Published: July 11, 2024

Language: Английский

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Near-Infrared Visualization of NAD(P)H Dynamics in Live Cells and Drosophila melanogaster Larvae Using a Coumarin-Based Pyridinium Fluorescent Probe

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

A near-infrared fluorescent probe,

Language: Английский

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Deep-Red and Near-Infrared Compact Cyanine Dyes for Sensitive NAD(P)H Sensing in Live Cells and Kidney Disease Tissues

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Cyanine dyes constructed for NAD(P)H near-infrared sensing utilize extended π-conjugation but often exhibit delayed fluorescence responses to due reduced positive charge density in 3-quinolinium acceptors. This study introduces deep-red and compact cyanine represented by probes A B mitochondrial detection live cells. Probes feature a unique structural design with double bond connection linking strategically positioned 1-methylquinolinium acceptor units at 2- 4-positions, correspondingly. Probe absorbs 359 531 nm, while probe 324 370 emitting subtle 587 628 respectively, no NADH present. Upon exposure, significant emission enhancements 612 656 correspondingly, attributed the efficient reduction of electron-donative 1-methyl-1,4-dihydroquinoline units. B, chosen its fast response NAD(P)H, effectively monitored dynamic intracellular levels throughout diverse experimental conditions. In HeLa cells, minimal basal increased upon stimulation. It also identified following chemical treatments acesulfame potassium, cisplatin, carboplatin, temozolomide, CoCl2-induced hypoxia, TLR4 activation macrophages disease models kidney pathology, where diseased tissues exhibited higher than normal tissues. fruit fly larvae under starvation conditions, tracked increases triggered exogenous NADH, demonstrating vivo applicability metabolic studies. These findings highlight B's utility elucidating fluctuations biological contexts, offering insights into function cellular metabolism.

Language: Английский

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A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, their roles in various physiological pathological processes. To address this need, we introduce sensor A, a near-infrared ratiometric fluorescent real-time, quantitative imaging fluctuations live cells. Sensor A combines 3-quinolinium electron-deficient acceptor with rhodamine dye, offering high sensitivity specificity superior photophysical properties. In its unbound state, emits strongly at 650 nm weakly 465 upon 400 excitation. Upon binding to NAD(P)H, it shows fluorescence increase decrease nm, enabling accurate measurements. also exhibits upconversion when excited 800 or 810 additional flexibility different experimental setups. sensor's response relies on the reduction by forming 1,4-dihydroquinoline donor that enhances quenches emission through photoinduced electron transfer. This mechanism ensures reliable quantification levels while minimizing interference from concentration, excitation intensity, environmental factors. was validated HeLa MD-MB453 cells under metabolic pharmacological conditions, including glucose maltose stimulation treatments chemotherapeutic agents. Co-localization mitochondrial-specific dyes confirmed mitochondrial targeting, precise tracking fluctuations. vivo Drosophila larvae nutrient starvation exposure revealed dose-dependent responses, highlighting potential changes organisms. represents significant advancement imaging, providing powerful tool exploring metabolism biology biomedical research.

Language: Английский

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Advances in activatable small-molecule fluorescent probes for recognizing cisplatin and related biomolecules during cisplatin treatment

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118189 - 118189

Published: Feb. 1, 2025

Language: Английский

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A near-infrared julolidine probe for visualization of mitochondrial peroxynitrite in living cells

Organic & Biomolecular Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The overproduction of peroxynitrite (ONOO-) in mitochondria has been associated with various pathophysiological conditions and disorders. However, the use fluorescent probes to visualize mitochondrial ONOO- biological systems is limited due their low emission wavelengths small Stokes shifts, which present significant challenges. In this study, we designed synthesized julolidine-based near-infrared (NIR) probes, named JQMe JCN, specifically monitor ONOO-. Comparative photophysical studies revealed that exhibits superior properties for sensing compared JCN. Initially, emitted fluorescence at 706 nm via an intramolecular charge transfer (ICT) mechanism. Upon addition ONOO-, NIR was suppressed, resulting a rapid on-off response within 5 minutes. exhibited high specific selectivity towards over other competing interferents, accompanied by colorimetric change from deep blue colorless. Additionally, shift 106 detection limit 6.5 nM. proposed mechanism validated through ESI mass spectrometry DFT studies. Furthermore, successfully employed both endogenous exogenous living cells using inducer inhibitor tests. Remarkably, time-dependent colocalization experiments effectively targets reacts

Language: Английский

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